Simultaneous quantification of haemoglobin adducts of ethylene oxide, propylene oxide, acrylonitrile, acrylamide and glycidamide in human blood by isotope-dilution GC/NCI-MS/MS

Schettgen, Thomas; Müller, Johannes; Fromme, Hermann; Angerer, Jürgen

doi:10.1016/j.jchromb.2009.11.043

Cited by 28 publications

(13 citation statements)

References 29 publications

(34 reference statements)

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“…CEV was thus used as a tool to reconstruct the exposure at the moment of the train accident. Indeed, the lifetime of the erythrocytes in the human body is long (126 days) and as there are no repair mechanisms for haemoglobin adducts, their quantification offers the unique possibility to explore even past high exposures or chronic low level exposures (Schettgen et al, 2010). The results found in this study are in excellent accordance with duration and assumed intensity of exposure, respectively.…”

Section: Discussionsupporting

confidence: 71%

Acrylonitrile exposure in the general population following a major train accident in Belgium: A human biomonitoring study

et al. 2014

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Section: Discussionsupporting

confidence: 71%

Acrylonitrile exposure in the general population following a major train accident in Belgium: A human biomonitoring study

et al. 2014

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“…In a study of 104 nonsmokers, higher levels of CyEtVal (formed by acrylonitrile) were found in 12 ETS‐exposed subjects ( p = 0.07) than in the 92 self‐reported unexposed subjects, while the levels of OHEtVal (from ethylene oxide), hydroxpropylvaline (from propylene oxide), AAVal (from acrylamide) and GAVal (from glycidamide) did not differ between exposed and unexposed groups 44…”

Section: Exposure To Etsmentioning

confidence: 93%

“…In a study of 104 nonsmokers, higher levels of CyEtVal (formed by acrylonitrile) were found in 12 ETS-exposed subjects (p ¼ 0.07) than in the 92 self-reported unexposed subjects, while the levels of OHEtVal (from ethylene oxide), hydroxpropylvaline (from propylene oxide), AAVal (from ac-rylamide) and GAVal (from glycidamide) did not differ between exposed and unexposed groups. 44 Comparisons of paired maternal blood and cord blood levels of hemoglobin adducts derived from acrylamide, glycidamide and ethylene oxide indicate that the placenta gives little protection to the fetus from exposure to these compounds in utero. 45 Nonsmoking mothers exposed to ETS during pregnancy had detectable levels of HPB-releasing hemoglobin adducts, as did the fetal cord blood samples analyzed.…”

Section: Exposure To Etsmentioning

confidence: 99%

DNA and protein adducts in human tissues resulting from exposure to tobacco smoke

Phillips

Venitt

2012

Intl Journal of Cancer

124

122

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Tobacco smoke contains a variety of genotoxic carcinogens that form adducts with DNA and protein in the tissues of smokers. Not only are these biochemical events relevant to the carcinogenic process, but the detection of adducts provides a means of monitoring exposure to tobacco smoke. Characterization of smoking‐related adducts has shed light on the mechanisms of smoking‐related diseases and many different types of smoking‐derived DNA and protein adducts have been identified. Such approaches also reveal the potential harm of environmental tobacco smoke (ETS) to nonsmokers, infants and children. Because the majority of tobacco‐smoke carcinogens are not exclusive to this source of exposure, studies comparing smokers and nonsmokers may be confounded by other environmental sources. Nevertheless, certain DNA and protein adducts have been validated as biomarkers of exposure to tobacco smoke, with continuing applications in the study of ETS exposures, cancer prevention and tobacco product legislation. Our article is a review of the literature on smoking‐related adducts in human tissues published since 2002.

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“…The procedure has had widespread applications for monitoring environmental and occupational exposure to alkylating agents. GC-MS/MS procedures (Paulsson et al, 2003;Schettgen et al, 2004bSchettgen et al, , 2010Urban et al, 2006;Berger et al, 2011) and HPLC-MS/MS procedures have been developed for the analysis of Hb adducts with AA and GA. A typical HPLC-MS/MS procedure involves precipitation of globin from red blood cells, which is then reacted with pentafluorophenyl isothiocyanate, followed by addition of stable isotope-labelled analytes as internal standards, SPE purification and ESI HPLC-MS/MS Tareke et al, 2006;Bjellas et al, 2007c;Huang et al, 2012). In the procedure of Vesper et al (2006) the analytes were isolated using liquid-liquid extraction on diatomaceous earth.…”

Section: Methodsmentioning

confidence: 99%

Scientific Opinion on acrylamide in food

Publication¹

2015

EFS2

337

170

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EFSA was asked to deliver a scientific opinion on acrylamide (AA) in food. AA has widespread uses as an industrial chemical. It is also formed when certain foods are prepared at temperatures above 120 °C and low moisture, especially in foods containing asparagine and reducing sugars. The CONTAM Panel evaluated 43 419 analytical results from food commodities. AA was found at the highest levels in solid coffee substitutes and coffee, and in potato fried products. Mean and 95th percentile dietary AA exposures across surveys and age groups were estimated at 0.4 to 1.9 µg/kg body weight (b.w.) per day and 0.6 to 3.4 µg/kg b.w. per day, respectively. The main contributor to total dietary exposure was generally the category 'Potato fried products (except potato crisps and snacks)'. Preferences in home-cooking can have a substantial impact on human dietary AA exposure. Upon oral intake, AA is absorbed from the gastrointestinal tract and distributed to all organs. AA is extensively metabolised, mostly by conjugation with glutathione but also by epoxidation to glycidamide (GA). Formation of GA is considered to represent the route underlying the genotoxicity and carcinogenicity of AA. Neurotoxicity, adverse effects on male reproduction, developmental toxicity and carcinogenicity were identified as possible critical endpoints for AA toxicity from experimental animal studies. The data from human studies were inadequate for dose-response assessment. The CONTAM Panel selected BMDL 10 values of 0.43 mg/kg b.w. per day for peripheral neuropathy in rats and of 0.17 mg/kg b.w. per day for neoplastic effects in mice. The Panel concluded that the current levels of dietary exposure to AA are not of concern with respect to non-neoplastic effects. However, although the epidemiological associations have not demonstrated AA to be a human carcinogen, the margins of exposure (MOEs) indicate a concern for neoplastic effects based on animal evidence. © European Food SUMMARYFollowing a request from the European Commission, the Panel on Contaminants in the Food Chain (CONTAM Panel) was asked to deliver a scientific opinion on acrylamide (AA) in food. The Panel developed the draft scientific opinion which underwent a public consultation from 1 July 2014 to 15 September 2014. The comments received and how they were taken into account when finalising the scientific opinion were published in an EFSA Technical Report (EFSA, 2015).AA is a low molecular weight, highly water soluble, organic compound. It is used inter alia as an industrial chemical and in the production of polyacrylamides. Heightened concerns about exposure to AA arose in 2002 when it was discovered that it forms when certain foods are prepared at temperatures usually above 120 °C and low moisture. It forms, at least in part, due to a Maillard reaction between certain amino acids, such as asparagine, and reducing sugars. However, several other pathways and precursors have also been proposed to contribute to AA formation. AA forms in numerous baked or fried carbohydrate-rich f...

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Simultaneous quantification of haemoglobin adducts of ethylene oxide, propylene oxide, acrylonitrile, acrylamide and glycidamide in human blood by isotope-dilution GC/NCI-MS/MS

Cited by 28 publications

References 29 publications

Acrylonitrile exposure in the general population following a major train accident in Belgium: A human biomonitoring study

Acrylonitrile exposure in the general population following a major train accident in Belgium: A human biomonitoring study

DNA and protein adducts in human tissues resulting from exposure to tobacco smoke

Scientific Opinion on acrylamide in food

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