Simultaneous Quantification of Five Stereoisomeric Hexoses in Nine Biological Matrices Using Ultrahigh Performance Liquid Chromatography with Tandem Mass Spectrometry

Zuo, Jian; Cai, Runxian; An, Yanpeng; Tang, Huiru

doi:10.1007/s41664-020-00142-z

Cited by 11 publications

(4 citation statements)

References 37 publications

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“…Because of the polarity of DNT (logP = −0.55) [17], liquid chromatography-ultraviolet detection (LC-UV) [18] and liquid chromatography-mass spectrometry (LC-MS) [19][20][21] are widely used techniques. In addition to the detection method, calibrants are also indispensable for accurate results.…”

Section: Introductionmentioning

confidence: 99%

Purity Assessment of Dinotefuran Using Mass Balance and Quantitative Nuclear Magnetic Resonance

Zhang

et al. 2023

Molecules

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Dinotefuran (DNT) belongs to the third-generation neonicotinoid pesticides, which are among the most common residuals in a variety of food commodities. To guarantee accurate quantification and traceability of results in food samples, certified reference materials (CRMs) are the indispensable benchmark. In this work, a DNT CRM was characterized and its purity was assessed by two independent methods, including mass balance (MB) and quantitative nuclear magnetic resonance spectroscopy (qNMR). The mass fraction of moisture was 0.33 mg/g, the inorganic impurity was 0.01 mg/g, and no detectable organic solvent was detected. Benzoic acid was chosen as the internal standard for qNMR. Its mass fraction was 997.9 mg/g and 992.9 mg/g by MB and qNMR, respectively. Eventually, the DNT CRM was assigned a mass fraction of 995 mg/g, with expanded uncertainty of 5 mg/g (k = 2). This CRM can be used to prepare calibrant solutions and is applicable to national routine monitoring of DNT residuals in agro-products and food.

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Section: Introductionmentioning

confidence: 99%

Purity Assessment of Dinotefuran Using Mass Balance and Quantitative Nuclear Magnetic Resonance

Zhang

et al. 2023

Molecules

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“…Liquid chromatography tandem mass spectrometry (LC-MS/MS) is undoubtedly a powerful tool for trace analyte detection due to its strong qualitative and quantitation ability [ 15 , 16 ]. By monitoring characteristic ion pairs of each analyte, LC-MS/MS allows one to detect targeted molecules with high specificity and sensitivity even in complex matrices [ 17 , 18 , 19 ]. Therefore, the use of the LC-MS/MS technique could eliminate the inherent drawbacks of FAA derivatization.…”

Section: Introductionmentioning

confidence: 99%

Development of a Simple, Underivatized Method for Rapid Determination of Free Amino Acids in Honey Using Dilute-and-Shoot Strategy and Liquid Chromatography-Tandem Mass Spectrometry

Ma,

Yang,

et al. 2022

Molecules

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A simple, fast and reliable analytical method was developed for 20 free amino acids (FAAs) determination in honey samples through a dilute-and-shoot strategy and hydrophilic interaction liquid chromatography tandem mass spectrometry. Compared with previous reports, direct dilution by water has significantly reduced the matrix effect and facilitated full extraction of FAAs. Further, a 5 min determination method was established with an acetonitrile–water mobile phase system with 0.1% formic acid addition. The established method was validated and demonstrated several advantages including short detection time, wide linear range over 3–4 orders of magnitude, high sensitivity down to 0.1 ng/mL and negligible matrix effect. Twenty FAAs were determined in 10 honey samples from different botanical origins by this method, and 19 FAAs were found. This general applicable method was also promising for fast determination of FAAs in other practical samples.

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“…The Michael addition uses 1-phenyl-3-methyl-5-pyrazolone (PMP) as a derivatization reagent for the analysis of reducing monosaccharides. − This method requires large amounts of PMP, and removal of the excess PMP by extractions with chloroform water could lead to the loss of derivatives during this process. Hydrazone formation-based derivatization with 3-nitrophenylhydrazine was applied to determine monosaccharides in red wine and biological matrices . In these studies, the acidic monosaccharides that widely exist in a variety of organisms were not included.…”

mentioning

confidence: 99%

“…Hydrazone formation-based derivatization with 3-nitrophenylhydrazine was applied to determine monosaccharides in red wine 22 and biological matrices. 23 In these studies, the acidic monosaccharides that widely exist in a variety of organisms were not included. Reductive amination introduces a group at the reducing terminus of carbohydrates using anthranilic acid 24 or 2aminobenzamide.…”

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confidence: 99%

Paired Derivatization Approach with H/D-Labeled Hydroxylamine Reagents for Sensitive and Accurate Analysis of Monosaccharides by Liquid Chromatography Tandem Mass Spectrometry

Wang

et al. 2022

Anal. Chem.

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Monosaccharides play important roles in biological processes. Sensitive and accurate analyses of monosaccharides remain challenging because of their high hydrophilicities and poor ionization efficiencies. Here, we developed a paired derivatization approach with H/D-labeled hydroxylamines for simultaneous quantification of 12 monosaccharides by liquid chromatography tandem mass spectrometry (LC–MS/MS). O-(4-Methoxybenzyl)hydroxylamine hydrochloride (4-MOBHA·HCl) showed higher derivatization efficiency for monosaccharides compared to six other hydroxylamine analogues. The derivatization of monosaccharides was readily achieved in an aqueous solution. Furthermore, the deuterium-labeled isotope reagent, d 3-4-MOBHA·HCl, was newly synthesized to stably label monosaccharides to improve its accuracy and precision in complex matrix analysis. As a result, 12 monosaccharides were rapidly detected by LC–MS/MS within 16 min with significant improvements in chromatographic separation and retention time. The detection sensitivity increased by 83 to 1600-fold with limits of quantitation ranging from 0.25 to 3.00 fmol. With the paired derivatization strategy, the monosaccharides could be accurately quantified with good linearity (R 2 > 0.99) and satisfactory accuracy (recoveries: 85–110%). Using this method, we achieved sensitive and accurate quantification of the monosaccharide composition of herbal polysaccharides and the change in monosaccharide levels in human cell lines under physiopathological conditions. More importantly, the developed method was able to differentiate between the levels of the monosaccharides in fecal samples of human ulcerative colitis (UC) patients and UC mice compared to their respective controls. The differential monosaccharides determined in human feces provided a good diagnostic performance in distinguishing the UC patients from healthy individuals, showing potential for clinical application.

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Simultaneous Quantification of Five Stereoisomeric Hexoses in Nine Biological Matrices Using Ultrahigh Performance Liquid Chromatography with Tandem Mass Spectrometry

Cited by 11 publications

References 37 publications

Purity Assessment of Dinotefuran Using Mass Balance and Quantitative Nuclear Magnetic Resonance

Purity Assessment of Dinotefuran Using Mass Balance and Quantitative Nuclear Magnetic Resonance

Development of a Simple, Underivatized Method for Rapid Determination of Free Amino Acids in Honey Using Dilute-and-Shoot Strategy and Liquid Chromatography-Tandem Mass Spectrometry

Paired Derivatization Approach with H/D-Labeled Hydroxylamine Reagents for Sensitive and Accurate Analysis of Monosaccharides by Liquid Chromatography Tandem Mass Spectrometry

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