Simultaneous quantification of five proteins and seven additives in dairy products with a heart-cutting two-dimensional liquid chromatography method

Hou, Xiaofang; Sun, Meng; He, Xin; Liang, Chen; Zhang, Ping

doi:10.1002/jssc.201500684

Cited by 12 publications

(5 citation statements)

References 36 publications

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“…The better peak shape and baseline could be obtained using 10 m m ammonium phosphate buffer. Because a short switching time in the 1 D column could improve the shape of the peaks in the second‐dimensional chromatogram (Hou et al, ), the corresponding mobile phases of first‐dimensional separation were ACN–ammonium phosphate buffer (20:80, v/v) to elute the VPA quickly. Under these conditions, VPA could be completely transferred within 2.02–3.00 min from 1 D column to the middle column.…”

Section: Resultsmentioning

confidence: 99%

A novel and nonderivatization method for the determination of valproic acid in human serum by two‐dimensional liquid chromatography

Liu

Shang

Yao

et al. 2019

Biomedical Chromatography

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A novel and robust two‐dimensional liquid chromatography with ultraviolet detection method (2D‐LC–UV) was developed and validated for high‐throughput determination of the concentrations of valproic acid (VPA) in human plasma. This 2D‐LC system was composed of a first‐dimensional LC column, a second‐dimensional LC column and an intermediate transfer column. The sample was directly injected into the 2D‐LC system after an easy protein precipitation treatment. After online preconcentration and primary separation by the first‐dimensional column, the target was captured by an intermediate column and then transferred to second‐dimensional column for analysis. The system transferred the target through “central cutting” mode whereby the drug peak was not subject to interference from the matrix. The analysis cycle time was completed within 7.0 min. Compared with other methods that have been developed, the analysis time was reduced and the operation was much easier without any derivatization. The calibration curve was linear over the 5.90–188.94 μg/ml range for the VPA concentrations. The intra‐day and inter‐day precisions were <5.6%. The recoveries were in the range from 95.2 to 98.0%. This method appears to be sensitive, precise, rapid and low‐cost for the quantification of VPA in serum sample.

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Section: Resultsmentioning

confidence: 99%

A novel and nonderivatization method for the determination of valproic acid in human serum by two‐dimensional liquid chromatography

Liu

Shang

Yao

et al. 2019

Biomedical Chromatography

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“…Therefore, a fully automated method was developed with the advantage of performing the whole process in less than 35 min and without requiring an additional sample preparation procedure thanks to the on‐line clean‐up effect. Other applications have also focused on the analysis of food additives in milk (Hou, Sun et al, 2015), milk powder (Ma, Zhang et al, 2014) and yogurt (Hou, Ma et al, 2015) by LC–LC methodologies. These latter studies share the use of trapping columns in the interface between 1 D and 2 D. The selection of the trapping columns must be carefully considered since they must be more retentive than the 1 D column but not too much to avoid carry over and to elute the analytes with the 2 D solvent.…”

Section: Application Of 2d‐lc–ms To Food Analysismentioning

confidence: 99%

Comprehensive and heart‐cutting multidimensional liquid chromatography–mass spectrometry and its applications in food analysis

Caño-Carrillo

Gilbert-López

Montero

et al. 2023

Mass Spectrometry Reviews

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In food analysis, conventional one‐dimensional liquid chromatography methods sometimes lack sufficient separation power due to the complexity and heterogeneity of the analyzed matrices. Therefore, the use of two‐dimensional liquid chromatography (2D‐LC) turns out to be a powerful tool to consider, especially when coupled to mass spectrometry (MS). This review presents the most remarkable 2D‐LC–MS food applications reported in the last 10 years, including a critical discussion of the multiple approaches, modulation strategies as well as the importance of the optimization of the different analytical aspects that will condition the 2D‐LC–MS performance. The presence of contaminants in food (food safety), the food quality, and authenticity or the relationship between the beneficial effects of food and human health are some of the fields in which most of the 2D‐LC–MS applications are mainly focused. Both heart‐cutting and comprehensive applications are described and discussed in this review, highlighting the potential of 2D‐LC–MS for the analysis of such complex samples.

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“…The figures in this chapter were taken from my previously published papers [6,23], and I have got the permission to reuse it.…”

Section: Acknowledgementsmentioning

confidence: 99%

Analysis of Additives in Milk Powders with SPE-HPLC or 2D-HPLC Method

Hou¹,

Ma²,

Liang³

et al. 2019

Milk Production, Processing and Marketing

Self Cite

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Dairy products are beneficial to human health, especially for formula-fed newborns. According to the regulation of FDA and China national food safety standard, food additives such as benzoic acid, sorbic acid, natamycin, lysozyme, saccharin sodium, and aspartame are not permitted to be added to milk powder. So, the establishment of accurate and convenient methods for the analysis of these food additives in milk powder is critical to people's health. For the reason of the complex matrix of infant milk powders, we compared six sample pretreatment methods (liquid-liquid extraction, organic precipitation, heavy precipitation, and three different solid-phase extraction (SPE) methods (C18, HLB, MAX)) from recovery, easy operation, time cost, and organic solvent usage aspects. Finally, Poly-Sery HLB cartridge was confirmed as the most appropriate material for its high recovery and time cost merits. We are also introducing two-dimensional liquid chromatography (2DLC) method for the simultaneous determination of five major proteins and seven food additives in milk powders. Optimization of switching mode, choice of columns, mobile phase, and flow speed was discussed. We also compared limit of detection (LOD), recovery, and sample treatment with the results of high-performance liquid chromatography (HPLC). Results show that 2DLC is simpler, faster, and more accurate than the HPLC method.

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Simultaneous quantification of five proteins and seven additives in dairy products with a heart-cutting two-dimensional liquid chromatography method

Cited by 12 publications

References 36 publications

A novel and nonderivatization method for the determination of valproic acid in human serum by two‐dimensional liquid chromatography

A novel and nonderivatization method for the determination of valproic acid in human serum by two‐dimensional liquid chromatography

Comprehensive and heart‐cutting multidimensional liquid chromatography–mass spectrometry and its applications in food analysis

Analysis of Additives in Milk Powders with SPE-HPLC or 2D-HPLC Method

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