Simultaneous quantification of fat-soluble vitamins A, 25-hydroxylvitamin D and vitamin E in plasma from children using liquid chromatography coupled to Orbitrap mass spectrometry

Liu, Hao; Wang, Dong-juan; Wan, Ke-Xing; Zhang, Juan; Yuan, Zhaojian; Yu, Chao-wen; Yang, Jing; Zou, Lin

doi:10.1016/j.jchromb.2021.122795

Cited by 12 publications

(10 citation statements)

References 24 publications

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“…As already reported by other authors, VA (Liu et al, 2021), 25‐OH VD3 (Ertugrul et al, 2020), 25‐OH VD2 (Albahrani et al, 2016; Thibeault et al, 2012) and VE (Liu et al, 2021) are efficiently ionized by ESI. No intact precursor ion has been observed because immediate loss of a water molecule occurred for VA (Rola et al, 2019).…”

Section: Resultssupporting

confidence: 70%

“…Compared with the published literature, we note that the simultaneous determination for FSVs is the trend, benefiting from the advantages of LC–MS/MS application. The proposed method adopted in the literature (Albahrani et al, 2016; Andreoli et al, 2004; Ertugrul et al, 2020; Liu et al, 2021) has enabled more than three compounds to be analyzed simultaneously, which was better than the method introduced by Rola and Hinchliffe (Hinchliffe et al, 2016; Rola et al, 2019). Protein precipitation is the traditional method used widely (Andreoli et al, 2004).…”

Section: Resultsmentioning

confidence: 99%

“…Protein precipitation is the traditional method used widely (Andreoli et al, 2004). However, a lower LOQ could be achieved using the PP and LLE procedure because the matrix effect could be reduced to the lowest level, and the analytes were concentrated in those procedures (Ertugrul et al, 2020; Liu et al, 2021). Some other sample preparation measures were also developed to treat samples, such as derivatization (Rola et al, 2019) or SLE (Hinchliffe et al, 2016), which employed multiple pretreatment procedures and was more complicated.…”

Section: Resultsmentioning

confidence: 99%

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A new method based on dispersive liquid–liquid microextraction for fat‐soluble vitamin determination in serum by LC–MS/MS

Chen

Sun

et al. 2023

Biomedical Chromatography

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A green and inexpensive pretreatment known as dispersive liquid–liquid microextraction (DLLME) was developed in this assay coupled with the LC–MS/MS method for routine analysis of fat soluble vitamins (FSVs). The technique was performed with methanol as the dispersive solvent and dichloromethane as the extraction solvent. The extraction phase containing FSVs was evaporated to dryness and reconstituted in a mixture of acetonitrile and water. The influence variables concerning the DLLME procedure were optimized. After that, the method was investigated for its applicability in LC–MS/MS analysis. As a result, the parameters were settled for the optimal conditions during the DLLME process. A cheap and lipid‐free substance was found as an alternative to serum to eliminate the matrix effect while preparing the calibrators. The method validation indicated that it was suitable for determining FSVs in serum. Moreover, this method was applied successfully to determine serum samples, which was consistent with the literature. In summary, the DLLME method developed in this report was reliable and more cost‐effective than the traditional LC–MS/MS method, and could be applied in the future.

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Section: Resultssupporting

confidence: 70%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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A new method based on dispersive liquid–liquid microextraction for fat‐soluble vitamin determination in serum by LC–MS/MS

Chen

Sun

et al. 2023

Biomedical Chromatography

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“…10−12 Several MSI methodologies, such as matrix-assisted laser desorption ionization (MALDI)-MSI and desorption electrospray ionization (DESI)-MSI, have been developed and are used widely to map lipids, 13 amino acids, 14 nucleotides, 15 sugars, 16 and vitamins. 17 Chemical derivatization is frequently used as a strategy for increasing ion yields, improving chromatographic performance, and facilitating structure identification in gas chromatography tandem-mass spectrometry and liquid chromatography-mass spectrometry (LC)-MS analysis. On-tissue chemical tagging of unionizable target analytes with easily ionized moieties is an alternative means of enhancing ionization efficiency, sensitivity, and specificity in MSI experiments.…”

Section: Introductionmentioning

confidence: 99%

“…Mass spectrometry imaging (MSI) is a powerful molecular imaging technique used to locate various known and unknown metabolites across biotissue sections. − Several MSI methodologies, such as matrix-assisted laser desorption ionization (MALDI)-MSI and desorption electrospray ionization (DESI)-MSI, have been developed and are used widely to map lipids, amino acids, nucleotides, sugars, and vitamins . However, visualizing poorly ionized and low-abundant metabolites using MSI remains challenging.…”

Section: Introductionmentioning

confidence: 99%

Mapping of Fatty Aldehydes in the Diabetic Rat Brain Using On-Tissue Chemical Derivatization and Air-Flow-Assisted Desorption Electrospray Ionization-Mass Spectrometry Imaging

et al. 2022

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Fatty aldehydes (FALs) are involved in various biological processes, and their abnormal metabolism is related to the occurrence and development of neurological diseases. Because of their low ionization efficiency, methods for in situ detection and mass spectrometry imaging (MSI) analysis of FALs remain underreported. On-tissue chemical tagging of hardly ionizable target analytes with easily ionized moieties can improve ionization efficiency and detection sensitivity in MSI experiments. In this study, an on-tissue chemical derivatization-air-flow-assisted desorption electrospray ionization-MSI method was developed to visualize FALs in the rat brain. The method showed high sensitivity and specificity, allowing the use of in situ high-resolution MS 3 to identify FALs. The methodology was applied to investigate the region-specific distribution of FALs in the brains of control and diabetic encephalopathy (DE) rats. In DE rats, FALs were found to be significantly enriched in various brain regions, especially in the cerebral cortex, hippocampus, and amygdala. Thus, increased FAL levels and oxidative stress occurred in a region-dependent manner, which may contribute to cognitive function deficits in DE. In summary, we provide a novel method for the in situ detection of FALs in biological tissues as well as new insights into the potential pathogenesis of DE.

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Analysis of vitamin D metabolic markers by mass spectrometry: Recent progress regarding the “gold standard” method and integration into clinical practice

Αλεξανδρίδου

Schorr

Stokes

et al. 2021

Mass Spectrometry Reviews

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Liquid chromatography/tandem mass spectrometry is firmly established today as the gold standard technique for analysis of vitamin D, both for vitamin D status assessments as well as for measuring complex and intricate vitamin D metabolic fingerprints. While the actual mass spectrometry technology has seen only incremental performance increases in recent years, there have been major, very impactful changes in the front‐ and back‐end of MS‐based vitamin D assays; for example, the extension to new types of biological sample matrices analyzed for an increasing number of different vitamin D metabolites, novel sample preparation techniques, new powerful chemical derivatization reagents, as well the continued integration of high resolution mass spectrometers into clinical laboratories, replacing established triple‐quadrupole instruments. At the same time, the sustainability of mass spectrometry operation in the vitamin D field is now firmly established through proven analytical harmonization and standardization programs. The present review summarizes the most important of these recent developments.

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Simultaneous quantification of fat-soluble vitamins A, 25-hydroxylvitamin D and vitamin E in plasma from children using liquid chromatography coupled to Orbitrap mass spectrometry

Cited by 12 publications

References 24 publications

A new method based on dispersive liquid–liquid microextraction for fat‐soluble vitamin determination in serum by LC–MS/MS

A new method based on dispersive liquid–liquid microextraction for fat‐soluble vitamin determination in serum by LC–MS/MS

Mapping of Fatty Aldehydes in the Diabetic Rat Brain Using On-Tissue Chemical Derivatization and Air-Flow-Assisted Desorption Electrospray Ionization-Mass Spectrometry Imaging

Analysis of vitamin D metabolic markers by mass spectrometry: Recent progress regarding the “gold standard” method and integration into clinical practice

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