Simultaneous quantification of acylated and desacylated ghrelin in biological fluids

Aydın, Süleyman; Karataş, Fikret; Geçkil, Hikmet

doi:10.1002/bmc.1065

Cited by 15 publications

(9 citation statements)

References 23 publications

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“…All aliquots were stored at −80°C until analyses. Prior to analyses, aliquots were thawed on ice, and milk fat was separated from the aqueous phase by centrifugation at 3000× g for 10 min . The resulting skimmed milk was assayed using commercially available immunoassay kits for insulin, leptin, IL‐6 and TNF‐α.…”

Section: Methodsmentioning

confidence: 99%

Relationship of insulin, glucose, leptin, IL‐6 and TNF‐α in human breast milk with infant growth and body composition

2012

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Numerous appetite, growth, obesity-related hormones and inflammatory factors are found in human breast-milk, but there is little evidence on their relationship with infant body composition. The purpose of the present cross-sectional pilot study was to assess the cross-sectional associations of appetite-regulating hormones and growth factors (leptin, insulin, glucose) and inflammatory factors (IL-6 and TNF-α) in human breast-milk with infant size, adiposity, and lean tissue at 1-month of age in healthy term infants. Human breast-milk was collected from nineteen exclusively breast-feeding mothers using one full breast expression between 8:00 and 10:00 am. The milk was then mixed, aliquoted, stored at −80°C and then centrifuged to remove the milk fat, prior to analyses using commercially available immunoassay kits; milk analytes were natural log transformed prior to analysis. Infant body composition was assessed using a Lunar iDXA v11-30.062 scanner (Infant whole body analysis enCore 2007 software, GE, Fairfield, CT). Maternal pre-pregnancy BMI was positively associated with milk leptin concentration (p=0.0027), and so maternal-BMI-adjusted Spearman correlations were examined between breast-milk analytes and infant growth and body composition variables. As previously reported, greater milk leptin was associated with lower BMIZ (r= −0.54, p=0.03). Glucose was positively associated with relateive weight (r = 0.6, p=0.01), and both fat and lean mass (0.43 – 0.44, p<0.10). Higher concentrations of milk insulin were associated with lower infant weight, relative weight, and lean mass (r = −0.49 – 0.58, p<0.06). Higher milk IL-6 was associated with lower relative weight, weight gain, percent fat, and fat mass (r = −0.55 – 0.70, p<0.03 for all), while higher TNF-α was associated with lower lean mass (r=−0.58, p=0.05), but not measures of adiposity. These preliminary data suggest for the first time that in the first months of life, breast-milk concentrations of insulin, glucose, IL-6 and TNF-α, in addition to leptin, may be bioactive and differentially influence the accrual of fat and lean body mass.

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Section: Methodsmentioning

confidence: 99%

Relationship of insulin, glucose, leptin, IL‐6 and TNF‐α in human breast milk with infant growth and body composition

2012

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“…Thus, immunoassays are extremely useful for quantification studies, but are not always applicable for the study of peptide and protein drug metabolism (Rose, Gaines Das, & Balen, 2000). For quantification studies antibodies' epitopes have to be specifically designed in order to distinguish between the various peptide and metabolites such as the example of acylated and desacylated ghrelin (Aydin, Karatas, & Geckil, 2008). In the case of parathyroid hormone (PTH) two‐site immunometric assays have been introduced, however the methodologies were characterized by cross‐reactivity (Worth et al, 2004).…”

Section: Methodsmentioning

confidence: 99%

Peptide and protein drugs: The study of their metabolism and catabolism by mass spectrometry

Κάτσιλα

Siskos

Tamvakopoulos

2011

Mass Spectrometry Reviews

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Peptide and protein drugs have evolved in recent years into mainstream therapeutics, representing a significant portion of the pharmaceutical market. Peptides and proteins exhibit highly diverse structures, broad biological activities as hormones, neurotransmitters, structural proteins, metabolic modulators and therefore have a significant role as both therapeutics and biomarkers. Understanding the metabolism of synthetic or biotechnologically derived peptide and protein drugs is critical for pharmaceutical development as metabolism has a significant impact on drug efficacy and safety. Although the same principles of pharmacokinetics and metabolism of small molecule drugs apply to peptide and protein drugs, there are few notable differences. Moreover, the study of peptide and protein drug metabolism is a rather complicated process which requires sophisticated analytical techniques, and mass spectrometry based approaches have provided the capabilities for efficient and reliable quantification, characterization, and metabolite identification. This review article will focus on the current use of mass spectrometry for the study of the metabolism of peptide and protein drugs.

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“…Since active form of ghrelin as compared with total ghrelin is essential in particular for biological [28] and endocrine activities [35] , and thus, is physiologically more crucial in terms of OCD [36] , active ghrelin measurements were chosen in the present study. Observed serum ghrelin level (144±41 pg/mL) in healthy dogs was in good accordance with the levels of 172±17 pg/mL and 117±42 pg/mL reported for healthy beagle dogs [37] and humans [38] , respectively.…”

Section: Discussionmentioning

confidence: 99%

Kompulsif Kuyruk Isıran Köpeklerin Serum Leptin ve Ghrelin Seviyeleri ve Serum Kortizol, Tiroid Hormonları, Lipidler, Homosistein ve Folik Asit İle İlişkileri

Yalçın¹,

Yılmaz²,

Özarda³

2017

Kafkas Univ Vet Fak Derg

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Citation of This ArticleYalcın E, Yilmaz Z, Ozarda Y: Serum leptin and ghrelin levels and their relationship with serum cortisol, thyroid hormones, lipids, homocysteine and folic acid in dogs with compulsive tail chasing. Kafkas Univ Vet Fak Derg, 23 (2): 227-232, 2017. DOI: 10.9775/kvfd.2016 AbstractThe aim of this study was to investigate serum leptin and ghrelin levels and their relations with circulating cortisol, thyroid hormones, lipids, homocysteine (Hcy) and folic acid in dogs with compulsive tail chasing (CTC). The material of this study consists of fifteen dogs with CTC and 15 healthy controls of various weights, breeds, ages of both sexes were enrolled in the study. CTC was diagnosed on the basis of the dog's behavioral history, clinical signs, and results of other medical assessments. None of the dogs were considered to have concurrent medical disease that would account for CTC. Dogs with CTC had a higher leptin (8.3±0.9 ng/mL vs 1.7±0.2 ng/mL, P<0.001) and lower ghrelin levels (74±7 pg/mL vs 144±41 pg/mL, P<0.05) than those of healthy controls. Serum cortisol, lipids (cholesterol, phospholipids and NEFA) and Hcy levels increased (P<0.05), whereas serum folic acid decreased (P<0.001) in dogs with CTC as compared with controls. Serum ghrelin correlated negatively with cholesterol (P<0.05), but serum leptin correlated positively with cholesterol, fT4, and phospholipids (P<0.05). These results suggest that serum leptin and ghrelin levels may bring up a new perspective on our understanding of the pathophysiological mechanisms associated with CTC. Serum levels of both hormones may be associated with serum levels of lipids and free T4. Keywords

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Simultaneous quantification of acylated and desacylated ghrelin in biological fluids

Cited by 15 publications

References 23 publications

Relationship of insulin, glucose, leptin, IL‐6 and TNF‐α in human breast milk with infant growth and body composition

Relationship of insulin, glucose, leptin, IL‐6 and TNF‐α in human breast milk with infant growth and body composition

Peptide and protein drugs: The study of their metabolism and catabolism by mass spectrometry

Kompulsif Kuyruk Isıran Köpeklerin Serum Leptin ve Ghrelin Seviyeleri ve Serum Kortizol, Tiroid Hormonları, Lipidler, Homosistein ve Folik Asit İle İlişkileri

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