Simultaneous quantification of 8-iso-prostaglandin-F2α and 11-dehydro thromboxane B2 in human urine by liquid chromatography–tandem mass spectrometry

A ultra‐pressure liquid chromatography/triple quadrupole tandem mass spectrometry method for the analysis of 13 eicosanoids in human urine and quantitative 24 hour values in healthy volunteers in a controlled constant diet

“…It involves the efficiency of the extraction procedure and is determined by spiking the baseline urine samples with known amounts of standards. The recovery was calculated as previously described . The ratio '[(final concentration – initial concentration)/added concentration]' was determined for three different concentrations between the lower and upper limits of the previously estimated linear range of the standards (0.2, 150, and 330 ng mL –1 ).…”

“…). The use of the Strata X‐AW, C18 Sep‐Pak, and Oasis‐HLB cartridges followed the protocols described by Taylor et al ., Cavalca et al ., and Zhang et al ., respectively. The eluted compounds were dried completely using a SpeedVac concentrator (Savant SPD121P, Thermo Scientific, MA, USA).…”

“…Schematic representation of the three distinct solid‐phase extraction procedures performed on three separate classes of extraction cartridges: Strata X‐AW (a), C18 Sep‐Pak (b), and Oasis HLB (c), corresponding to the references Taylor et al ., Cavalca et al ., and Zhang et al ., respectively.…”

New UHPLC–QqQ-MS/MS method for quantitative and qualitative determination of free phytoprostanes in foodstuffs of commercial olive and sunflower oils

“…It involves the efficiency of the extraction procedure and is determined by spiking known amounts of standards to the baseline urine samples. Recovery was calculated as previously described (Cavalca et al, 2010;Medina et al, 2012). The ratio '[(final concentration À initial concentration)/added concentration]' was determined in two different levels of concentrations (15-16 ng/mL and 30-32 ng/mL depending on the metabolite) between the lower and upper limits of the previously estimation of the standards linearity.…”

Assessing Free‐Radical‐Mediated DNA Damage during Cardiac Surgery: 8‐Oxo‐7,8‐dihydro‐2′‐deoxyguanosine as a Putative Biomarker

“…8-Iso-PGF 2 α determination was performed by modifying a previously described LC-MS/MS method [24]. Briefly, the chromatographic separation was performed using an XBridge® C18 column (100 × 2.1 mm, particle size 3.5  μ m; Waters Milford, MA, USA) maintained at 30°C.…”