Simultaneous quantification of 11 isoquinoline alkaloids in Corydalis impatiens (Pall.) Fisch by HPLC

Abstract

Isoquinoline alkaloids are the primary active ingredients of Corydalis, but an analytical method for quality assessment of the active ingredients in Corydalis impatiens (Pall). Fisch has not been reported. A new, simple, and multiple-component quantification method was developed for the simultaneous quantification of 11 isoquinoline alkaloids including capnoidine, chelianthifoline, bicuculline, protopine, isoapocavidine, apocavidine, cavidine, tetrahydroepiberberine, ochotensimine, tetrahydrocoptisine, and tetrahydrocorysamine in C. impatiens. Separation of the isoquinoline alkaloids was performed on a RP C18 column (150 × 4.6 mm, 5 μm) with potassium dihydrogen phosphate buffer (pH 2.5, adjusted by phosphoric acid)/acetonitrile (53:47, v/v) containing 0.3% sodium dodecyl sulfonate. The flow rate and detection wavelength were set at 1 mL/min and 295 nm, respectively. Full validation of the assay was carried out including linearity, precision, accuracy, stability, LOD, and limit of quantitation. All calibration curves showed a good linear relationship (r > 0.999) in test range. The results demonstrated that the developed method was reliable, rapid, and specific. Six batches of C. impatiens samples from different sources were determined using the established method. The contents of alkaloids ranged from 11.68 to 351.83 μg/g. This method can be applied for quality evaluation and control of C. impatiens. Eleven isoquinoline alkaloids were first reported on simultaneous determination with HPLC.

Analysis of the traditional medicine YiGan San by the fragmentation patterns of cadambine indole alkaloids using HPLC coupled with high‐resolution MS

“…Sample stability of 48 h was examined by repeatedly injecting the same sample solution every 24 h. A recovery test was then used to assess the accuracy of the method. Three concentration levels of reference substances (approximately equivalent to 80, 100, and 120% levels of target compounds) were added with two parallels at each level and analyzed in the same way . The isolation and purification procedure is summarized in the Supporting Information.…”

Rapid and sensitive liquid chromatography with tandem mass spectrometry method for the simultaneous determination of 11 major components of Yuanhu-Baizhi herb-pair in rat perfusion fluids

“…There are several publications on determining alkaloids in Yuanhu or coumarins in Baizhi , but no method regarding simultaneous quantification of eight alkaloids and three coumarins from the herb pair in situ using an LC–MS/MS method is available. Wan et al reported the simultaneous determination of three coumarins in 12 min and Gao et al published their method for assaying four alkaloids simultaneously in 22 min.…”

“…Several analytical methods were reported for the determination of some components in Yuahu‐Baizhi herb‐pair individually or in other combinations in pharmaceutical formulations and in biological matrices. However, there was no method available for the simultaneous determination of major active components in rat intestinal perfusion fluids.…”

Liquid chromatographic separation of alkaloids in herbal medicines: Current status and perspectives

“…Quality assessment of isoquinoline alkaloids in herbs of Corydalis impatiens (Pall.) Fisch was implemented with mobile phase containing SDS [43]. In addition, TFA can also be considered as an effective ion‐pairing reagent [44].…”