Simultaneous qualitative and quantitative analysis of 28 components in <i>Isodon rubescens</i> by HPLC‐ESI‐MS/MS

Du, Yingfeng; Liu, Pengwei; Yuan, Zhifang; Jin, Yiran; Zhang, Xiaowei; Sheng, Xiaona; Shi, Xiaowei; Wang, Qiao; Zhang, Lantong

doi:10.1002/jssc.200900704

Cited by 23 publications

(12 citation statements)

References 24 publications

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“…Concerning the immediate precision, the %RSD values of the retention times and peak areas were between 3-4% and 1-2%, respectively. These results are similar to those described by Du et al (2010) and Gobbo-Neto and Lopes (2008) for phenolic compounds in Lamiaceae and Asteraceae plants, respectively. Since the %RSD values were lower than 5%, it is possible to conclude that the chromatographic instrument presents a satisfactory precision (Ermer & Miller, 2005).…”

Section: Hplc Methods -Validation and Quantification Of Phenolic Composupporting

confidence: 91%

Simultaneous characterization and quantification of phenolic compounds in Thymus x citriodorus using a validated HPLC–UV and ESI–MS combined method

Pereira

Peres

Silva

et al. 2013

Food Research International

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Thymus x citriodorus is a Lamiaceae plant extensively cultivated in the Mediterranean region and used for centuries in culinary and in traditional medicine. The present work describes the detailed phenolic composition of T. x citriodorus for the first time, by means of HPLC-DAD, ESI-MS and MS n and nuclear magnetic resonance (NMR) analyses. The ethanolic extract of T. x citriodorus was analyzed by reversed phase HPLC. The method of analysis was also validated concerning its linearity, instrumental and method precision (for repeatability, immediate precision and intermediate precision) and accuracy (absolute recovery study). The technique was combined with electrospray mass spectrometry in order to identify the phenolic compounds and the structure of the main phenolics was also confirmed by NMR analysis. The in-house validated HPLC-DAD method showed good linearity for the tested reference compounds as well as satisfactory repeatability and immediate precision values, for both instrument and method. Furthermore, the satisfactory results of intermediate precision analysis and recovery assays indicated that the chromatographic method could be used to quantify the main phenolic compounds of T. x citriodorus with adequate precision and accuracy. The extract was rich in rosmarinic acid (10.4 ± 0.6 mg/g extract) that is a widespread phenolic acid in Thymus plants, but also in luteolin-7-O-α-glucuronide (12 ± 2 mg/g extract), that was herein reported in Thymus for the first time. Other novel compounds comprised one eriodictyol dihexoside with O-glycosidic linkages, two eriodictyol-O-monohexosides, one quercetagetin dimethyl ether-O-hexoside, one naringenin-O-hexoside and chrysoeriol-7-β-O-glucoside. Having in mind the health-promoting properties reported in literature for some of the main polyphenols found in T. x citriodorus, we suggest that this plant has a high potential for being used as a functional food.

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Section: Hplc Methods -Validation and Quantification Of Phenolic Composupporting

confidence: 91%

Simultaneous characterization and quantification of phenolic compounds in Thymus x citriodorus using a validated HPLC–UV and ESI–MS combined method

Pereira

Peres

Silva

et al. 2013

Food Research International

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“…First, for developing the method, the optimization of precursor ions and product ions of the analytes for MRM mode analysis by syringe pump infusion at a flow rate of 10 μl/min was a prerequisite for successful quantification[18]. The standard solutions of the analytes were infused into the mass spectrometer separately to obtain MS/MS fragment ions and to optimize mass parameters such as DP and CE.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous quantification of six major flavonoids from Fructus sophorae by LC-ESI-MS/MS and statistical analysis

Zhang

Ren

et al. 2013

Indian J Pharm Sci

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A new, sensitive and selective high-performance liquid chromatography–tandem mass spectrometric method has been developed for the determination of six major flavonoids including sophoricoside, genistin, genistein, rutin, quercetin, kaempferol in Fructus sophorae. Principal component analysis and hierarchical clustering analysis were used to classify and differentiate these samples. Chromatographic separation was performed on a C18 column with linear gradient elution of methanol and 0.05% acetic acid (v/v) at a flow rate of 0.8 ml/min. The detection was accomplished in the negative mode using multiple-reaction monitoring. The total run time was 8.0 min. Full validation of the assay was carried out including linearity, precision, accuracy, recovery, limit of detection and limit of quantification. The validated method was successfully applied to the simultaneous determination of these active components in Fructus sophorae. The results demonstrated that the quantitative difference in content of six active compounds was useful for chemotaxonomy of many samples from different sources and the standardization and differentiation of many similar samples. Simultaneous quantification of bioactive components by high-performance liquid chromatography–tandem mass spectrometric method coupled with chemometric techniques would be a well-acceptable strategy to comprehensively control the quality of Fructus sophorae.

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“…The global optimization was started with parameters of a population of 100 randomly positioned individuals. The maximum number of energy evaluations was increased to 2.5×10 7 and the maximum number of generations in the Lamarckian genetic algorithm was increased to 2.7×10 5 . The Solis and Wets local search was performed with a maximum number of 3,000.…”

Section: Molecular Dockingmentioning

confidence: 99%

Aqueous extract of Rabdosia rubescens leaves: forming nanoparticles, targeting P-selectin, and inhibiting thrombosis

Wang¹,

Tang²,

Zhu³

et al. 2015

IJN

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The hot water extract of Rabdosia rubescens was traditionally used as an antithrombotic medicine. To explore its antithrombotic utility and mechanism, we carried out a series of in vitro and in vivo assays in this study. In vitro platelet aggregation assay showed that the half maximal inhibitory concentration values of aqueous extract of R. rubescens leaves (AERL) inhibiting platelet aggregation induced by thrombin, arachidonic acid, adenosine diphosphate, and platelet-activating factor ranged from 0.12 mg/mL to 1.43 mg/mL. The minimal effective oral dose of AERL inhibiting the rats from forming thrombus was 25 mg/kg. Both in vitro and in vivo actions were correlated with AERL concentration-dependently inhibiting sP-selectin release. In water, AERL formed nanoparticles, and their size depended on the concentration. Docking the five nucleotides, 21 phenolic acids, and four diterpenoids identified by high-performance liquid chromatography–photodiode array detector/(−)electrospray ionization-tandem mass spectrometry analysis into the active site of P-selectin, rosmarinic acid was predicted to be the antithrombotic ingredient of AERL. In flow cytometry analysis, 1 μM of rosmarinic acid effectively inhibited sP-selectin release in arachidonic acid-activated platelets. In a rat model, 5 mg/kg of oral rosmarinic acid effectively inhibited thrombosis.

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Simultaneous qualitative and quantitative analysis of 28 components in Isodon rubescens by HPLC‐ESI‐MS/MS

Cited by 23 publications

References 24 publications

Simultaneous characterization and quantification of phenolic compounds in Thymus x citriodorus using a validated HPLC–UV and ESI–MS combined method

Simultaneous characterization and quantification of phenolic compounds in Thymus x citriodorus using a validated HPLC–UV and ESI–MS combined method

Simultaneous quantification of six major flavonoids from Fructus sophorae by LC-ESI-MS/MS and statistical analysis

Aqueous extract of Rabdosia rubescens leaves: forming nanoparticles, targeting P-selectin, and inhibiting thrombosis

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