Simultaneous Qualitative Analysis of Coumarins in the Rhizome of Angelica polymorpha Maxim. by LC–DAD–ESI–MS

Abstract

A novel method has been developed for simultaneous qualitative analysis of coumarins in the rhizome of Angelica polymorpha Maxim. with the use of high-performance liquid chromatography (LC)-photodiode array detection (DAD)-electrospray ionization (ESI)-tandem mass spectrometry (MS). LC analysis was performed on an Agilent Eclipse XDB-C 18 analytical column with a gradient solvent system of water and methanol, ultraviolet (UV) absorption detection was at 300 nm and peak UV spectrum and (?) ESI-MS data were acquired by DAD and a quadrupole mass spectrometer, respectively. Eight peaks were identified as linear furocoumarins including aviprin, isopimpinellin, bergapten, isooxypeucedanin, gosferol, imperatorin, phellopterin and isoimperatorin by comparing their retention time, UV, and MS spectra with that of the authentic standards, and the analysis of UV spectra and ESI-MS data of the standards gave the characteristics of UV absorption and ESI-MS fragmentation. On the basis of the above characteristics, the possible structures of four other linear furocoumarins were deduced in LC. The other chemical structures were proposed as three angular furocoumarins, a simple coumarin, two chromones, and a cinnamic acid by comparing their UV and MS data with that of literature reports. Accordingly, the method we established can simply and accurately identify the key chemical constituents in the rhizome of A. polymorpha.

Imperatorin–pharmacological meaning and analytical clues: profound investigation

“…Linear furanocoumarins, including imperatorin, show four areas of absorption, at 205–225, 240–255, 260–270, and 298–316 nm (Murray and Mendez 1982 ; Skalicka-Woźniak and Głowniak 2014 ). UV λ max values obtained from the HPLC–DAD analysis of imperatorin are 217.9, 248.8, 263.5, and 302.9 nm (Liu et al 2011 ), or 218.1, 248.7, and 300.9 nm (Kim et al 2011 ). The characteristic UV–DAD spectra for imperatorin are presented in Fig.…”

“…Characteristic values for the positive mass spectrometry ions [M+H] + and [M+Na] + of imperatorin are observed at m / z 271.1 and 293.0, respectively. Masses of the main fragment ions occur at m / z 203.1 and 147.2 (Liu et al 2011 ). The mass spectral fragmentation pattern for imperatorin is presented in Fig.…”

Qualitative and quantitative analysis of nine major compounds in the Bozhougyiqi-Tang using a high-performance liquid chromatography coupled with a diode array detector and electrospray ionization mass spectrometer

“…[11] Intra-day variation was determined by analyzing 5 replicates within 1 day, and inter-day variation was assayed in 5 replicates for 3 sequential days (1,3,5 days). Variations were expressed by relative standard deviation (RSD).…”

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

“…41 coumarins at trace concentration were identified in a crude extract of Radix Glehniae. In another work, Liu et al applied LC-DAD-QqQ-MS in the positive-ion mode to determine coumarin derivatives in the rhizomes of Angelica polymorpha [128]. UV absorption characteristics of linear furocoumarins were given close attention to facilitate differentiation of coumarins with different substitution.…”