Simultaneous Profiling of 17 Steroid Hormones for The Evaluation of Endocrine-Disrupting Chemicals in H295R Cells

Abstract

The developed approach could be beneficial for the mechanistic investigation of EDCs.

The use of purified rat Leydig cells complements the H295R screen to detect chemical-induced alterations in testosterone production†

“…Numerous investigators have utilized the H295R cell line as a rapid in vitro screen to evaluate toxicant-induced effects on adrenocortical steroidogenesis [14][15][16][17]. The H295R steroidogenesis assay has been validated by both the EPA (EDSP Test Guideline 890.1550) and the Organization for Economic Cooperation and Development (OECD) (OECD Test Guideline 456) [18] for the assessment of two major reproductive hormones, i.e., 17βestradiol and testosterone.…”

Methods and Techniques for Metabolic Phenotyping

“…However, except in the case of, for example, breath (or other) volatiles, the need for carefully optimized derivatization in order to modify otherwise involatile metabolites and facilitate their analysis by GC is a key requirement for success and this aspect is considered from a practical standpoint in another article in this issue [11]. The use of a targeted GC-MS method to obtain 'steroidomic' data on the cellular response of a range of steroid hormones to the in vitro exposure of H295R cells (of proven value in screening for chemicals that affect steroid biosynthesis) to endocrine disruptors therefore provides a timely example of the application of this technology to gain insights into the changes wrought on cellular steroidogenic pathways by such chemicals [12].…”