Simultaneous production of laccase and degradation of bisphenol A with Trametes versicolor cultivated on agricultural wastes

Abstract

Solid state fermentation with Trametes versicolor was carried out on agricultural wastes containing bisphenol A (BPA). It was found that BPA degradation was along with the occurrence of laccase production, and wheat bran and corn straw were identified as suitable mixed substrates for laccase production. In the process of BPA degradation with T. versicolor, laccase activity increased rapidly at the 6th–10th day after inoculation. Moreover, BPA can enhance the production of laccase. After 10 days of fermentation, degradation rate of BPA exceeded 90% without the usage of mediators ABTS and acetosyringone at pH 4.0–8.0. In addition, metal ions did not affect the BPA degradation with T. versicolor. In vitro, the optimum pH range of BPA degradation with laccase was in the acidic region with the optimal performance of pH 5.0. Metal ions Cu2+, Zn2+, and Co2+ showed little effect on BPA degradation. However, Fe3+ and Fe2+ substantially inhibited the BPA degradation. Natural mediator acetosyringone showed optimum enhancement on BPA degradation. Greater than 90% of the estrogenic activity of BPA was removed by T. versicolor and its laccase. Compared to in vitro degradation with laccase, this study shows that the process of simultaneous laccase production and BPA degradation with T. versicolor was more advantageous since BPA can enhance the laccase production, mediators were unnecessary, degradation rate was not affected by metal ions, and the applicable pH range was broader. This study concludes that T. versicolor and laccase have great potential to treat industrial wastewater containing BPA.

Simultaneous laccase production and transformation of bisphenol-A and triclosan using Trametes versicolor

“…Calculation of transformation using HPLC analysis was based on the equation (Eq. 2) given by (Zeng et al 2017) Results and discussion…”

“…Flasks were sterilized by autoclaving at 121 °C and 100 15 psi for 15 min. After cooling the flasks to room temperature (~ 25 °C), they were inoculated with 2 mL of fungal inoculum followed by an incubation at 30 °C for 20 days (Zeng et al 2017).…”

“…The effects of synthetic and natural mediators in all the three formats (SSF, SmF and free enzyme) were studied. All the enzymatic reactions were carried out as described above at room temperature with 0.5 mM of synthetic 1-hydroxybenzotriazole (HBT) or vanillin as natural mediator dissolved in the CP buffer (pH 4.4), before the inoculation (Zeng et al 2017).…”

Fungal Laccase Production from Lignocellulosic Agricultural Wastes by Solid-State Fermentation: A Review

“…Bisphenol A (BPA) degradation was found to be accompanied by laccase production, and BPA can induce laccase. During the degradation of BPA by T. versicolor under SSF, laccase activity increased rapidly from day 6 to day 10 compared with the untreated control [6]. In addition, laccase-producing fungi were also used to remove other phenolic compounds.…”

“…They were first described in Toxicodendron vernicifluum (Japanese lacquer tree; formerly called Rhus vernicifera) and subsequently in a wide variety of organisms, including bacteria, insects, and fungi (notably, white rot fungi) [3]. Laccases display broad substrate specificity and are applied in many industrial and environmental technology areas, including in textile effluents (decolorization, detoxification), paper production (biobleaching, biopulping), and biopharmaceuticals (transformation of antibiotics, steroids) [4][5][6]. Their ability to remove xenobiotic substances and generate polymeric products makes them useful in bioremediation processes [7,8].…”