Simultaneous production of bioethanol and value-added d-psicose from Jerusalem artichoke (Helianthus tuberosus L.) tubers

Song, Younho; Oh, Chihoon; Bae, Hyeun‐Jong

doi:10.1016/j.biortech.2017.08.079

Cited by 34 publications

(15 citation statements)

References 23 publications

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“…Besides, its yield potential and low requirements meant that it could be of interest in the energy sector (Kowalczyk-Juśko et al 2012). Recently, rapidly growing interest is for the use of Jerusalem artichoke tubers, which are rich in inulin, as raw materials for bioethanol production (Celp et al 2012;Song et al 2017). Jerusalem artichoke also has potential for generating a variety chemicals, such as citric acid, 2,3-butanediol (Liu et al 2010), butyric acid (Huang et al 2011) and sorbitol (Wei et al 2001).…”

Section: Stemsmentioning

confidence: 99%

Phytochemicals in bioenergy crops

2019

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The use of natural compounds derived from agricultural crops and other plants as health promoting chemicals gains tremendous growing interest in various industrial sectors as well as among people worldwide. These chemicals have been more and more employed by the food industry as food additives, functional food ingredients, nutraceuticals, by feedstuffs industry, but also by the cosmetic and pharmaceutical industries. The general idea for this interest is to use natural products as potential alternatives to synthetic chemicals. On the other hand, some plants characterized by high yield and being used as energy crops also contained significant amount of bioactive compounds. This review focuses on the wide spectrum of the phytochemicals present in available biomass plants. It is supposed that extraction of bioactive chemicals from energy crops before their energetic use may increase economical effectiveness, providing simultaneously a double benefit in the form of phytochemicals and bioenergy as value added products. This remains in line with bioeconomy, which is defined by European Commission as ''the production of renewable biological resources and the conversion of these resources and waste streams into value added products, such as food, feed, bio-based products and bioenergy''. However, the issue is still a challenging effort due to the high costs, technology readiness and regulatory hurdles.

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Section: Stemsmentioning

confidence: 99%

Phytochemicals in bioenergy crops

2019

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“…The D-allulose and ethanol that were produced were separated to obtain value-added pure D-allulose. 19 However, in our study, inulin was converted into a mixed sugar syrup containing D-allulose, D-fructose, and small amount of D-glucose. After concentration, this syrup could be used directly as a functional sweetener and would be suitable for people suffering from diabetes or obesity.…”

Section: Immobilization For the Fusion Enzymementioning

confidence: 60%

“…The dried JA was ground into 0.35 mm particles. 19 After mixing the JA powder and water in a ratio of 1:14, 75 g kg −1 cellulase (Merck, Darmstadt, Germany) was added and hydrolyzed at 50°C for 150 min. The mixed solution was centrifuged (12 000 × g for 5 min) and filtered to obtain the extract containing inulin.…”

Section: Extraction Of Inulinmentioning

confidence: 99%

“…18 Enzyme technology has also become the mainstream technology in the production of D-allulose. Research has shown that D-fructose hydrolyzed from JA by pectinase and inulinase can be utilized to produce D-allulose in multiple steps. 19 Inulinase is a member of glycoside hydrolase family 32 (GH32), which is widely used in pharmaceuticals, foods, and bioethanol production. 20,21 Inulinase can be divided into exo-inulinase (E.C.3.8.1.80) and endo-inulinase (E.C.3.2.1.7) based on different hydrolytic behavior towards glycoside bonds.…”

Section: Introductionmentioning

confidence: 99%

“…Enzyme technology has also become the mainstream technology in the production of d ‐allulose. Research has shown that d ‐fructose hydrolyzed from JA by pectinase and inulinase can be utilized to produce d ‐allulose in multiple steps 19 …”

Section: Introductionmentioning

confidence: 99%

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Fusion and secretory expression of an exo‐inulinase and a d‐allulose 3‐epimerase to produce d‐allulose syrup from inulin

et al. 2020

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BACKGROUND: This study developed a feasible catalytic method for D-allulose syrup production using a fusion enzyme, either in free or immobilized form, through hydrolysis of inulin extracted from Jerusalem artichoke tubers. RESULTS: D-Allulose 3-epimerase (DAE) was actively expressed in secretory form by fusing with the extracellular exo-inulinase CSCA in Escherichia coli BL21 (DE3). The best linker ligating the two enzymes was a flexible peptide containing 12 residues (GSAGSAAGSGEF). At 55°C and pH 8.0, and as with the addition of 1 mmol L −1 Mn 2+ , the CSCA-linkerE-DAE fusion enzyme obtained through high cell-density cultivation displayed a maximal exo-inulinase activity of 21.8 U mg −1 and resulted in a yield of 6.3 g L −1 D-allulose and 39.2 g L −1 D-fructose using 60 g L −1 inulin as the raw material. Catechol-modified alginate with titanium ions (Alg(Ti)PDA) was found to be a promising immobilization material for the fusion enzyme. After conversion for 8 days, the Alg(Ti)PDA-immobilized CSCA-linkerE-DAE (8 U g −1) completed 24 reaction cycles and retained over 80% of its original activity. Each reaction obtained an average of 19.8 g L −1 D-allulose and 32.7 g L −1 D-fructose from 60 g L −1 inulin. CONCLUSION: This study shed light on a feasible and cost-effective approach for the production of syrup containing D-allulose and D-fructose with inulin as the raw material via the use of a CSCA and DAE fusion enzyme. This syrup is of added value as a functional sweetener.

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