Simultaneous production of antibodies against T3 and T4 in a single animal; their characteristics and usefulness in radioimmmunoassay

Mehta, M.N.; Joseph, Lebana J.; Desai, K.B.

doi:10.1016/0883-2897(89)90076-7

Cited by 1 publication

(1 citation statement)

References 6 publications

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“…In order to prepare a monoclonal antibody against multifarious SEs, simultaneous immunization was attempted by some researchers during the process of cell fusion [18][19][20]. Meyer [21] obtained a monoclonal antibody capable of binding to SEA, SEB, SEC 1 , SED, and SEE by alternately injecting BABL/c mice with SEs serotypes of A and D. Then Shinagawa [22] reported that two monoclonal antibodies reacting with SEA, SEB, SEC 1 , SED, and SEE were obtained by immunizing BABL/c mice with five serotypes of SEs.…”

Section: Introductionmentioning

confidence: 99%

Production of a Monoclonal Antibody by Simultaneous Immunization of Staphylococcal Enterotoxin A and B

Liang

Zhang

Liu

et al. 2011

Appl Biochem Biotechnol

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In this paper, a method of simultaneous immunizing BALB/c mice with staphylococcal enterotoxin (SE) A and B (SEA and SEB) to prepare a monoclonal antibody (3F2) for detecting both of SEA and SEB was developed. The results showed that antibody 3F2 had high titers against both SEA and SEB by enzyme-linked immunosorbent assay (ELISA). The sensitivities of 3F2 to SEA and SEB detected by ELISA were 133.2 and 82.5 ng/mL, respectively, and the detection limits for the two enterotoxins were about 1 ng/mL. The antibody 3F2 had high specificities and affinities to both SEA and SEB, and had no cross-reaction with SEC(1), bovine serum albumin, and ovalbumin. SEs-free skimmed milk samples were spiked with different concentrations of SEA, SEB, or both of them, respectively. Average recoveries of SEA and SEB from the spiked samples were all nearly between 82% and 104%. The result suggested that one cell fusion with simultaneous immunization by multiple antigen to prepare monoclonal antibody against them was possible, simple, and economic. The monoclonal antibody could be used in simultaneous detecting multifarious SEs.

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