Simultaneous Production of Amyloglucosidase and Exo-Polygalacturonase by Aspergillus niger in a Rotating Drum Reactor

Colla, Eliane; Santos, Lucielen Oliveira; Deamici, Kricelle Mosquera; Magagnin, Glênio; Vendruscolo, Michele; Costa, Jorge Alberto Vieira

doi:10.1007/s12010-016-2237-y

Cited by 20 publications

(5 citation statements)

References 37 publications

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“…Our results related to the capacity of various mold species belonged to Aspergillus for the extracellular lipase production are in agreement with those previously founded [44]. A. niger [45] and A. flavus [46] were reported to be greater producer of the extracellular lipase. The accumulation and the increase of FFA content inside inoculated cocoa beans are due to the production of extracellular lipase by these mold isolates.…”

Section: Ffa (%)supporting

confidence: 93%

Effect of Spoilage by Lipolytic Fungi Strains on Free Fatty Acids (FFA) Formation in Fermented and Dry Cocoa Beans

Stéphanie,

Richard,

Maï

et al. 2023

MRJI

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Aim: High free fatty acids (FFA) content of cocoa beans is high lead to alter quality of butter fraction and chocolate manufactured. During primary post-harvest processing, cocoa beans harbor lipase producing mold speciess could trigger the accumulation of FFA. This study investigated the improvement of the quality of raw cocoa material sourced from Côte d’Ivoire regarding its high FFA concentration. Methods: Fermented and dry cocoa were sampled on-farm level in some main cocoa producing regions of Côte d'Ivoire. Contaminating mold strains were isolated using Sabouraud chloramphenicol agar incubated at 25°C for 7 days. Morphological identification of mold isolates was preformed from younger fungal culture. Biochemical study about lipolytic properties of mold isolates was carried out using Cazpeck-Dox Agar medium enriched with olive oil as carbon source and rhodamine B as indicator of lipase activity. Extraction, PCR amplification using a specific transcribed spacer primer (ITS4/ITS5) and sequencing of DNA were performed for fungal species molecular identification. Results: Approximately 154 mold isolates belonging to 8 genera were found in tested cocoa beans. Only Rhizopus oryzae, Absidia corymbifera, Mucor heimalis, Aspergillus niger, A. tubingensis, A. aculeatus, A. flavus, A. tamari, A. fumigatus, Nigrospora sphaerica, Curvularia geniculate and Penicillium chrysogenum species produced greater lipolytic properties. A. corymbifera, A. niger, A. fumigatus and R. orysae were the most frequent and predominant species. Cocoa beans inoculated with R. oryzae, A. corymbifera or A. niger recorded higher FFA content above 1.75%. Conclusion: Reducing of the mold species growth during cocoa primary post-harvest processing could sustainably produce raw cocoa material with less FFA.

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Section: Ffa (%)supporting

confidence: 93%

Effect of Spoilage by Lipolytic Fungi Strains on Free Fatty Acids (FFA) Formation in Fermented and Dry Cocoa Beans

Stéphanie,

Richard,

Maï

et al. 2023

MRJI

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“…In addition, the output pumps were discarded, and the effluent left the reactor by overflow. Fungal RDBs have been previously used for enzyme production (Colla et al, 2017;Domínguez et al, 2001) and biological desulfurization (Şener et al, 2018), and a similar rotating drum biological contactor of white-rot fungus has been applied for decolorization (Šíma et al, 2016). Recently, rotating biological contactors containing immobilized T. versicolor were implemented to remove pharmaceutical micropollutants from urban wastewaters (Cruz del Álamo et al, 2020).…”

Section: Rotating Drum Bioreactor Treating Diuron In Spiked Real Wast...mentioning

confidence: 99%

The removal of diuron from agricultural wastewaters by Trametes versicolor immobilized on pinewood in simple channel reactors

Beltrán-Flores

Torán

Caminal

et al. 2020

Science of The Total Environment

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“…For Bicentenario tricale malts, β-amylase yields ranged from 201. 13 2). However, the activity of β-amylase in the extracts could be conditioned by the availability of total solids (substrate), which was negatively correlated (-41%, P = 0.17).…”

Section: β-Amylase From Triticale Malts Aqueous Extractsmentioning

confidence: 99%

Recovery of amylolytic enzymes from triticale malt (X. Triticosecale Wittmack) using two-phase aqueous systems

Girón-Orozco

Berasain

Aguilar

et al. 2023

Preprint

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Introduction: Triticale malt has shown higher amylolytic activity than other cereal malts, a characteristic of great importance for the brewing and starch industry. The scope of this work was to obtain concentrated enzymatic aqueous extracts containing β-amylase, α-amylase, and amyloglucosidase from triticale malts of Bicentenario and Siglo-XXI varieties, using aqueous two-phase systems (ATPS) for enzymes recovery. Methodology: The malts produced had 5 days of germination and were dried at 50°C. The extracts were prepared by mixing ground malt with deionized water (1:10), stirred at 180 rpm, testing three stirring times (30, 120, and 270 min) and two temperatures (30 and 40°C) (12 treatments). The extracts were centrifugated, freeze-dried and purified, varying the concentrations of alcohol and Na3C6H5O7. Enzyme quantification was performed using: BETAMYL-3® (β-amylase); AOAC 2002.0I, AACC22-02.0I (α-amylase), and McCleary et al., (1991) (amyloglucosidase). Results: In relation to the malt, an increase in extract enzyme quantification was achieved, mainly α-amylase for Siglo-XXI malt, initially with 99.56 CU/g to 1,268.89 CU/mL (507.5 CU/g dry matter) in 30 min/30°C extract. For the same treatment, the best ATPS for enzymatic recovery was 30% alcohol/18% Na3C6H5O7, α-amylase predominantly at the inferior phase 1,514.03 CU/mL (605.6 CU/g dry matter) and β-amylase 51.43 BU/mL (10.2 BU/g dry matter) at the upper phase. Conclusion: Aqueous amylase extraction from triticale Siglo-XXI malt in 30 min/30°C conditions is a suitable option for production of β-amylase and α-amylase in combination with the use of ethanol and Na3C6H5O7 ATPS to recovery amylolytic enzymes.

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Simultaneous Production of Amyloglucosidase and Exo-Polygalacturonase by Aspergillus niger in a Rotating Drum Reactor

Cited by 20 publications

References 37 publications

Effect of Spoilage by Lipolytic Fungi Strains on Free Fatty Acids (FFA) Formation in Fermented and Dry Cocoa Beans

Effect of Spoilage by Lipolytic Fungi Strains on Free Fatty Acids (FFA) Formation in Fermented and Dry Cocoa Beans

The removal of diuron from agricultural wastewaters by Trametes versicolor immobilized on pinewood in simple channel reactors

Recovery of amylolytic enzymes from triticale malt (X. Triticosecale Wittmack) using two-phase aqueous systems

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