Simultaneous non-thermal saccharification of cassava pulp by multi-enzyme activity and ethanol fermentation by Candida tropicalis

Cassava Pulp as a Biofuel Feedstock of an Enzymatic Hydrolysis Proces

“…Cellulose (8.1±1.1%) and hemicellulose (2.8±0.1%) contents were lower than those reported previously [7] but lignin content (2.2±0.2%) was comparable to those reported previously [7,8]. Glucans were the major polysaccharide [8] while major monosaccharide of the cassava a pulp analysis was galactose (78%), and successively followed by arabinose (10.7%), rhamnose (6.2%), glucose (5.1%), xylose (2.5%), fuccose (0.7%) [15].…”

“…This combination consists of 0.724 U/g cellulase, 1.458 U/g hemicellulase, 4.109 U/g pectinase and 5.702 U/g amylase. The DE of this study was higher than those were achieved by Sriroth [4] and Thongchul [14] but lower than those were achieved by Rattanachomsri [7]. Addition of mixture of different enzymes (2 U/g cellulase, 1.6 U/g hemicellulase, 4.9 U/g pectinase and …”

“…The cassava pulp was dried below the starting gelatinization temperature of cassava starch to preserve the physical characteristics of the starch granules [11], hemicelluloses [12] and lignin [13]. Fibers content 20.1% of cassava pulp which is reported in this study was lower than previous reported by Kosugi [8], Rattanachomsri [7], Sriroth [4], Thongchul [14] which were 29%, 23.0%, 27.75% and 35.9%, respectively.…”

“…According to Sriroth [4] the combined action of cellulase (4) and pectinase (6) yielded more reducing sugar owing to the efficient hydrolysis of the non-starch fibrous structure and the cementing pectin containing compounds. On the other hand, addition of commercial β-glucanase /hemicellulase (such as 1) to the enzyme mix did not appreciably increase the fermentable sugar yield [7]. This might be due to the presence of high hemicellulase side activity of the commercial cellulase.…”

“…Enzymatically assist-depolimerization of the lignocellulosic cell wall materials with non-starch polysaccharide degrading commercial enzymes enable to liberate the starch granules from the polysaccharides entrapment and then starch degradation as well. Currently, focal point of ethanol production from feedstock of cassava pulp [4][5][6][7] is studied wether using hydrothermal process or its combination with acid pretreatment [2,[8][9]. Sriroth and coworkers [4] reported that enzymatic pretreatment with mixture of cellulase and pectinase also increased releasing starches from secondary cell wall entrapment.…”