Simultaneous nitrification and denitrification without nitrite accumulation by a novel isolated Ochrobactrum anthropic LJ81

Lei, Xin; Jia, Yating; Chen, Yuancai; Hu, Yongyou

doi:10.1016/j.biortech.2018.10.060

Cited by 126 publications

(40 citation statements)

References 46 publications

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“…The nitrogen removal rates decreased significantly after the logarithmic phase. This phenomenon was in agreement with the previous reports [17]. The reason might be that the nitrogen removal rates were inhibited by products released from bacteria, so that the concentrations of these inhibitors increased rapidly during the logarithmic phase due to the rapid growth of the bacteria, resulting in significant decreases in the removal rates.…”

Section: Discussionsupporting

confidence: 93%

“…When ammonia, nitrite, or nitrate were used as the sole nitrogen sources, strain SC16 showed a high removal efficiency and fast removal rate for all three inorganic nitrogen forms. The final removal rate of NO − 3 -N by strain SC16 was 4.15 mg•L −1 h −1 , higher than that by Pseudomonas tolaasii Y-11 (1.99 mg•L −1 h −1 ) [25] and Klebsiella pneumoniae CF-S9 (2.2 mg•L −1 h −1 ) [26], and similar to that by Ochrobactrum anthropi LJ81 (4.16 mg•L −1 h −1 ) [17]. The final removal rate of ammonium by strain SC16 was 5.05 mg•L −1 h −1 , higher than that of some other denitrifiers such as P. stutzeri T13 (3.98 mg•L −1 h −1 ) [27] and O. anthropi LJ81 (3.84 mg•L −1 h −1 ) [17].…”

Section: Discussionmentioning

confidence: 63%

“…The final removal rate of NO − 3 -N by strain SC16 was 4.15 mg•L −1 h −1 , higher than that by Pseudomonas tolaasii Y-11 (1.99 mg•L −1 h −1 ) [25] and Klebsiella pneumoniae CF-S9 (2.2 mg•L −1 h −1 ) [26], and similar to that by Ochrobactrum anthropi LJ81 (4.16 mg•L −1 h −1 ) [17]. The final removal rate of ammonium by strain SC16 was 5.05 mg•L −1 h −1 , higher than that of some other denitrifiers such as P. stutzeri T13 (3.98 mg•L −1 h −1 ) [27] and O. anthropi LJ81 (3.84 mg•L −1 h −1 ) [17]. In addition, the removal rate of nitrite (8.33 mg•L −1 h −1 ) was higher than those for nitrate and ammonium when SC16 was incubated with the three nitrogen sources, and the removal rate of nitrite by SC16 was also higher than those of Pseudomonas mendocina TJPU04 (1.85 mg•L −1 h −1 ) [28] and Arthrobacter arilaitensis Y-10 (0.19 mg•L −1 h −1 ) [29].…”

Section: Discussionmentioning

confidence: 63%

“…In addition, the removal rate of nitrite was much higher than that of nitrate, and the accumulation of nitrite was lower than that of nitrate during the removal of ammonia, meaning that there might be partial nitrification-denitrification, i.e., NH + 4 →NO − 2 →NO→N 2 O→N 2 [17]. The reduction of nitrite by NIR is the rate-limiting process of denitrification [37].…”

Section: Discussionmentioning

confidence: 99%

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Isolation and Characterization of an Aerobic Denitrifier Bacillus sp. SC16 from an Intensive Aquaculture Pond

Sang¹,

Fu²,

Guo³

et al. 2020

Water

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Overloading of ammonia and nitrite nitrogen in aquaculture can result in toxicity to aquatic animals. In order to eliminate the hazardous substances, a highly efficient denitrifying bacterium, Bacillus sp. SC16, was identified in a fishery pond and isolated subsequently. The strain SC16 could remove nitrate up to 97%, ammonia up to 36.6%, and nitrite up to 99.99% when incubated with nitrate at an initial concentration of 306.9 mg·L−1 for 72 h, ammonia at 165.49 mg·L−1 for 48 h, and nitrite at 200 mg·L−1 for 24 h under aerobic conditions. The nitrite reductase gene was identified as the nirK gene. The transcriptional levels of the nirK gene in strain SC16 incubated with ammonia, nitrate, and nitrite showed similar expression patterns. When the strain SC16 was used to treat the aquaculture water, the concentration of ammonia decreased significantly, from 8.35 mg·L−1 to 4.56 mg·L−1, and there was almost no accumulation of nitrite by the end of experiment. Therefore, the results indicated that Bacillus sp. SC16 could be a promising candidate for aquaculture water treatment.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Isolation and Characterization of an Aerobic Denitrifier Bacillus sp. SC16 from an Intensive Aquaculture Pond

Sang¹,

Fu²,

Guo³

et al. 2020

Water

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“…Ochrobactrum anthropi , a Gram-negative, heterotrophic bacterium is abundant in the environment. Various strains of O. anthropi have been recently isolated from many environmental compartments globally, for example, activated sludge collected from WWTPs in China [ 34 , 37 ], microbial mats collected from the delta region of the Ebro River [ 31 ] or sediment harvested from the surrounding of former chemical factory in Slovakia [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluating the Effect of Azole Antifungal Agents on the Stress Response and Nanomechanical Surface Properties of Ochrobactrum anthropi Aspcl2.2

2020

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Azole antifungal molecules are broadly used as active ingredients in various products, such as pharmaceuticals and pesticides. This promotes their release into the natural environment. The detailed mechanism of their influence on the biotic components of natural ecosystems remains unexplored. Our research aimed to examine the response of Ochrobactrum anthropi AspCl2.2 to the presence of four azole antifungal agents (clotrimazole, fluconazole, climbazole, epoxiconazole). The experiments performed include analysis of the cell metabolic activity, cell membrane permeability, total glutathione level and activity of glutathione S-transferases. These studies allowed for the evaluation of the cells’ oxidative stress response to the presence of azole antifungals. Moreover, changes in the nanomechanical surface properties, including adhesive and elastic features of the cells, were investigated using atomic force microscopy (AFM) and spectrophotometric methods. The results indicate that the azoles promote bacterial oxidative stress. The strongest differences were noted for the cells cultivated with fluconazole. The least toxic effect has been attributed to climbazole. AFM observations unraveled molecular details of bacterial cell texture, structure and surface nanomechanical properties. Antifungals promote the nanoscale modification of the bacterial cell wall. The results presented provided a significant insight into the strategies used by environmental bacterial cells to survive exposures to toxic azole antifungal agents.

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Insights into simultaneous microbial chromium and nitrate reduction: inhibitory effects and molecular mechanisms

Chen

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: The potential effect of chromium on the denitrification process has drawn much attention recently. The efficient remediation of groundwater contaminated by both hexavalent chromate [Cr(VI)] and nitrate (NO 3 − ) was hampered due to the deficiency of denitrification in the presence of Cr(VI). The goal of this work was to understand the mechanisms of how Cr(VI) affected denitrification. RESULTS:To better understand the underlying nature, Cr(VI) effects on denitrification were investigated in batch. Negative effects of Cr(VI) exposure on denitrification were confirmed and ascribed to restriction of bacterial activities, by regulating functional genes expression and altering community composition. Stronger inhibition of the expression of denitrifying genes was observed with increased Cr(VI) loading (0-50 mg L −1 ). The critical inhibitory concentration and IC 50 of Cr(VI) on electron transport system activities were calculated to be 3.21 and 4.87 mg L −1 , respectively. Further amplicon analysis revealed that Betaproteobacteria were enriched, implying their potential key role in simultaneously removing nitrate and Cr(VI). The presence of Cr(VI) also upregulated the metabolic activities related to apoptosis. CONCLUSION: These findings shed light on the biogeochemical fates of Cr(VI) and NO 3− in aquifers, and may contribute in enhancing their practical application for bioremediation using microbial processes. ETSA assayElectron transport activity of the bacteria was measured by reducing 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium wileyonlinelibrary.com/jctb

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Simultaneous nitrification and denitrification without nitrite accumulation by a novel isolated Ochrobactrum anthropic LJ81

Cited by 126 publications

References 46 publications

Isolation and Characterization of an Aerobic Denitrifier Bacillus sp. SC16 from an Intensive Aquaculture Pond

Isolation and Characterization of an Aerobic Denitrifier Bacillus sp. SC16 from an Intensive Aquaculture Pond

Evaluating the Effect of Azole Antifungal Agents on the Stress Response and Nanomechanical Surface Properties of Ochrobactrum anthropi Aspcl2.2

Insights into simultaneous microbial chromium and nitrate reduction: inhibitory effects and molecular mechanisms

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