Simultaneous nitrification and denitrification by diverse Diaphorobacter sp.

Abstract

Eight bacterial isolates closely related to Diaphorobacter sp. were isolated from activated biomass surviving on wastewater laden with dyes and nitro-substituted chemicals and were identified by 16S rDNA sequence analysis. The isolates showed sequence similarity of 99-100% to other Diaphorobacter strains such as ZY 2006b, F2, NA5, PCA039, D. nitroreducens KSP4, and KSP3 and 98-99% sequence homology to D. nitroreducens NA10B (type strain JCM 11421). Neighbor-joining tree revealed that all the eight strains formed tight cluster together and also showed close clustering with other Diaphorobacter strains. Isolates demonstrated the ability to perform simultaneous nitrification and denitrification under aerobic conditions. Strains HPC 805, 815, 821, and 856 gave highest chemical oxygen demand removal (85-93%) and ammonia removal (92-96%), which correlated well with higher growth rates of the cultures. Simultaneously, complete removal of nitrate supplied in the medium in presence of ammonium and acetate (electron donor) was observed in addition to aerobic nitrite release from ammonium. Thus, the above strains showed ability to perform partial nitrification followed by further aerobic removal of common intermediate nitrite, which indicated their potential application in treatment systems for treatment of high-nitrogen-containing wastewaters.

Removal of High-Strength of Ammonium and Phenol from Coking Wastewater by Alcaligenes faecalis No.4

“…Characteristics of No.4 have been previously described in details [11]. Cultured cells of No.4 were mixed with a 50% glycerol solution in vials and stored at -84°C.…”

“…When high-strength ammonium is involved in CW, COD in the wastewater is not sufficient to complete the removal of ammonium [1][2][3][4][5]. Recently, many bacteria have been known to be capable of heterotrophic nitrification and aerobic denitrification [6][7][8][9][10][11][12][13][14]. The use of these bacteria provides advantages over the conventional nitrogen removal process of aerobic nitrification and an anaerobic denitrification because ammonium removal is achieved in one reactor using one type of bacteria under aerobic conditions.…”

Nitrate removal performance of Diaphorobacter nitroreducens using biodegradable plastics as the source of reducing power

“…Khardenavis et al [29] reported that Diaphorobacter strains isolated from activated sludge were capable of simultaneous nitrification and denitrification under aerobic conditions. In this respect, it is interesting to investigate whether D. nitroreducens is capable of heterotrophic nitrification.…”

Heterotrophic nitrification and aerobic denitrification at low nutrient conditions by a newly isolated bacterium, Acinetobacter sp. SYF26

“…3b). Richardson et al (1998) indicated that the heterotrophic nitrification process and aerobic denitrification process are (Chen et al, 2012;Khardenavis et al, 2007;Robertson et al, 1988). In comparison, for strain SYF26, conversion of nitrate was observed during the process of ammonium removal, and both nitrite and nitrate could be denitrified efficiently under aerobic conditions.…”

“…Heterotrophic nitrifying-aerobic denitrifying micro-organisms capable of nitrification and denitrification simultaneously under aerobic conditions have drawn increasing attention (Chen & Ni, 2011;Khardenavis et al, 2007;Zhu et al, 2012). To date, certain groups of heterotrophic nitrification-aerobic denitrification micro-organisms, such as Paracoccus denitrificans (formerly known as Thiosphaera pantotropha), Alcaligenes faecalis, Pseudomonas stutzeri, Microvirgula aerodenitrificans and Bacillus have been isolated from soils and wastewater treatment systems (Kim et al, 2008;Yang et al, 2011;Joo et al, 2006).…”