Simultaneous nitrification and denitrification by novel heterotrophs in remediation of fish processing effluent

Abstract

Three isolates viz. Lysinibacillus sp. HT13, Alcaligenes sp. HT15 and Proteus sp. HT37 isolated from fish processing effluent and having a C/N ratio of 2, removed 218, 169, and 400 µg cell(-1) day(-1) NH4(+)-N, respectively without subsequent build up of nitrite or nitrate. Ability of the selected isolates in removing NH4(+)-N, NO2(-)-N, and NO3(-)-N was checked in the presence of four commonly reported and tested effluent carbon sources viz. pyruvate, glycerol, methanol, and acetate. Further, when supplemented to fish processing wastewater containing 234 ppm total Kjeldahl's nitrogen, Lysinibacillus sp. HT13, Alcaligenes sp. HT15, and Proteus sp. HT37 could remediate 95.74, 86.17, and 76.6% nitrogen, respectively in 48 h. This is the first report of a Lysinibacillus sp. carrying out aerobically the process of simultaneous nitrification and denitrification. The results demonstrate the potential of the isolates for use in treatment of fish processing effluents and demonstrating the efficient removal of ammonia.

Effective remediation of fish processing waste using mixed culture biofilms capable of simultaneous nitrification and denitrification

“…a and d). Planktonic stage had exhibited relatively higher residual nitrite and nitrate presence (reported previously by us in ) than in MBBR stage after 24 h of incubation (Fig. b and c).…”

“…The mixed consortium biofilm on carriers showed highest removal of ammonia and lowest production of nitrite and nitrate (p-value, Ã < 0.05) than carriers with individual bacterial biofilms. With respect to the individual remediation capability of the isolates used in planktonic stage (reported previously by us in [8]) and their biofilms on carriers (Fig. 1b), the mixed biofilms exhibited higher denitrification of effluents and minimum residual nitrogenous byproducts.…”

“…HT15 and Proteus sp. HT37 could remove 95.74, 86.17, and 76.59% total nitrogen from fish processing effluent, respectively, in 48 h. After 24 h of treatment of the fish processing waste water, the resultant removal of total nitrogen by isolates in planktonic stage (reported previously by us in ) was much less than them being used in biofilm of the MBBR (Fig. a and d).…”

Significance and Roles of Proteus spp. Bacteria in Natural Environments

“…Similar metabolic activity was displayed by the Proteus sp. strain related to P. mirabilis , isolated from effluents from a fish processing plant in India [86]. Simultaneous aerobic nitrification and denitrification leading to the efficient removal of nitrogen by this heterotrophic bacterium are suggested to be applied in fish waste treatment.…”

Bacterial communities in the rhizosphere of Phragmites australis from an oil-polluted wetland

“…For instance, the genera Ochrobactrum, Agrobacterium and Brevundimonas are known to include nitrogen-fixing species. Sequences related to nitrifiers such as Labrenzia, Bacillus and Lysinibacillus (Mishra et al 2015) and denitrifiers such as Thiobacillus and Pseudomonas (Ruiz-Rueda et al 2009) have also been encountered. Earlier studies have reported the presence of these bacterial taxa (Haaijer et al 2006) and the occurrence of nitrogen cycle processes such as N2 fixation, nitrification and denitrification (Whitmire & Hamilton 2005) in other wetlands.…”