Simultaneous multiple analyte detection using fluorescent peptides and capillary isoelectric focusing

Cruickshank, Kenneth A.; Olvera, Joe; Müller, Uwe R.

doi:10.1016/s0021-9673(98)00399-9

Cited by 16 publications

(21 citation statements)

References 6 publications

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“…53,2001 All commercial ampholytes were developed for conventional gel IEF in which this property is irrelevant. In clEF, a detection wavelength must be selected at which there is minimal interference from the ampholytes but satisfactory signal for proteins.…”

Section: Ampholyte Selectionmentioning

confidence: 99%

“…Acetic acid provided best resolution of human plasma proteins, particularly for Chromatographia Supplement Vol. 53,2001 acidic species. However, the use of lowmobility organic anions almost doubled the mobilization time.…”

Section: Two-step Clef With Electrophoretic Mobilizationmentioning

confidence: 99%

“…Capillaries coated internally with poly(viChromatographia Supplement Vol. 53,2001 nylalcohol) were used in this study, and pressure mobilization was compared to electrophoretic mobilization. The desalting technique was applied to clEF analysis of human blood and cerebrospinal fluid samples.…”

Section: For Clefmentioning

confidence: 99%

See 2 more Smart Citations

Recent Advances in Capillary Isoelectric Focusing

Wehr

Rodríguez-Díaz

Zhu

2001

CE in Biotechnology: Practical Applications for Protein and Peptide Analyses

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Section: Ampholyte Selectionmentioning

confidence: 99%

Section: Two-step Clef With Electrophoretic Mobilizationmentioning

confidence: 99%

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Recent Advances in Capillary Isoelectric Focusing

Wehr

Rodríguez-Díaz

Zhu

2001

CE in Biotechnology: Practical Applications for Protein and Peptide Analyses

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“…The concentration by focusing aspect of cIEF, which allows for the entire capillary to be filled with sample, enables LODs in the pm range. The simultaneous multiple detection of 11 different focusing entities was achieved [60].…”

Section: Rhodamine-based Dyesmentioning

confidence: 99%

Laser‐induced fluorescence detection schemes for the analysis of proteins and peptides using capillary electrophoresis

et al. 2005

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Over the past few years, a large number of studies have been prepared that describe the analysis of peptides and proteins using capillary electrophoresis (CE) and laser-induced fluorescence (LIF). These studies have focused on two general goals: (i) development of automatic, selective and quick separation and detection of mixtures of peptides or proteins; (ii) generation of new methods of quantitation for very low concentrations (nm and subnanomolar) of peptides. These two goals are attained with the use of covalent labelling reactions using a variety of dyes that can be readily excited by the radiation from a commonly available laser or via the use of noncovalent labelling (immunoassay using a labelled antibody or antigen or noncovalent dye interactions). In this review article, we summarize the works which were performed for protein and peptide analysis via CE-LIF.

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“…And CIEF continues to play an increasingly important role in the rapidly developing field of proteomics. There are three detection schemes in CIEF including single-point detection [17,18], whole-column scanning detection [19], and whole-column imaging detection [20][21][22]. Single-point detection is commonly used, and it needs a mobilization step to electroosmotically [23][24][25], hydrodynamically [26,27], or chemically [28,29] migrate the focused sample bands past the detection point.…”

Section: Introductionmentioning

confidence: 99%

On-line chemiluminescence detection for isoelectric focusing of heme proteins on microchips

Huang

Ren

2005

Electrophoresis

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In this paper we present a sensitive chemiluminescence (CL) detection of heme proteins coupled with microchip IEF. The detection principle was based on the catalytic effects of the heme proteins on the CL reaction of luminol-H2O2 enhanced by para-iodophenol. The glass microchip and poly(dimethylsiloxane) (PDMS)/glass microchip for IEF were fabricated using micromachining technology in the laboratory. The modes of CL detection were investigated and two microchips (glass, PDMS/glass) were compared. Certain proteins, such as cytochrome c, myoglobin, and horseradish peroxidase, were focused by use of Pharmalyte pH 3-10 as ampholytes. Hydroxypropylmethylcellulose was added to the sample solution in order to easily reduce protein interactions with the channel wall as well as the EOF. The focused proteins were transported by salt mobilization to the CL detection window. Cytochrome c, myoglobin, and horseradish peroxidase were well separated within 10 min on a glass chip and the detection limits (S/N=3) were 1.2x10(-7), 1.6x10(-7), and 1.0x10(-10) M, respectively.

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Simultaneous multiple analyte detection using fluorescent peptides and capillary isoelectric focusing

Cited by 16 publications

References 6 publications

Recent Advances in Capillary Isoelectric Focusing

Recent Advances in Capillary Isoelectric Focusing

Laser‐induced fluorescence detection schemes for the analysis of proteins and peptides using capillary electrophoresis

On-line chemiluminescence detection for isoelectric focusing of heme proteins on microchips

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