Simultaneous metabolite fingerprinting of hydrophilic and lipophilic compounds in Echinacea pallida by high-performance liquid chromatography with diode array and electrospray ionization-mass spectrometry detection

Pellati, Federica; Orlandini, Giulia; Benvenuti, Stefania

doi:10.1016/j.chroma.2012.04.025

Cited by 20 publications

(22 citation statements)

References 24 publications

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“…Two other caffeic acid derivatives, caftaric acid and cichoric acid , were also found in the roots of E. pallida with much lower content (Brown et al 2010 ). Also, HPLC-MS analysis also confi rmed that echinacoside is the most abundant derivative in E. pallida roots (Pellati et al 2012 ).…”

Section: Caffeic Acid Derivatives In Different Echinacea Speciesmentioning

confidence: 90%

The Medicinal Chemistry of Echinacea Species

Cao

Kindscher

2016

Echinacea

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Section: Caffeic Acid Derivatives In Different Echinacea Speciesmentioning

confidence: 90%

The Medicinal Chemistry of Echinacea Species

Cao

Kindscher

2016

Echinacea

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“…This procedure may improve genetic engineering of Echinacea species. Pellati et al (2012) b Based on references by Thomsen et al (2012) …”

Section: Resultsmentioning

confidence: 99%

“…The injection volume was 20 μl. The fractions of caftaric acid, cichoric acid and echinacoside were collected using HPLC and further analyzed by LC-MS (Pellati et al 2012;Thomsen et al 2012).…”

Section: High-performance Liquid Chromatography and Mass Spectrometrymentioning

confidence: 99%

“…High-performance liquid chromatography (HPLC) analysis was conducted using an Agilent 1200 Chemstation HPLC system and a C18 reverse-phase column (YMC-Pack Polymer C18, YMC, Japan) as described (Wang et al 2015). Phenolic compounds were monitored at 332 nm, and the mobile phase consisted of 0.1% formic acid in H 2 O (A) and acetonnitrile (B), and separations were performed using the following solvent gradient: 0 (10% B), 2 (15% B), 12 (30%B), 20 (65% B), 27 (80% B), 32 (90% B), and 50 min (10% B) (Pellati et al 2012). The injection volume was 20 μl.…”

Section: High-performance Liquid Chromatography and Mass Spectrometrymentioning

confidence: 99%

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In vitro regeneration, Agrobacterium-mediated transformation, and genetic assay of chalcone synthase in the medicinal plant Echinacea pallida

Wang

Jeng

2017

Plant Cell Tiss Organ Cult

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“…The field of metabolomics is now routine for the identification of primary and secondary metabolites in plant taking advantage of mass spectrometry (MS) [3][4][5][6] and for reaching a metabolic fingerprinting in plant tissues by both nuclear magnetic resonance (NMR) spectroscopy [7] and MS [8]. Fingerprinting is often used as a complementary tool to differentiate genetically modified species from pools of modified species [9], or to follow changes in the entire metabolism brought about by a specific treatment [10].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical profiling of tomato roots following treatments with different microbial inoculants as revealed by IT-TOF mass spectrometry

Nebbioso¹,

Martino

Eltlbany

et al. 2016

Chem. Biol. Technol. Agric.

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Background: In light of the growing interest for eco-compatible fertilization, tomato plant roots were treated with four different strains of microorganisms (B1-B4) capable of positively affecting plant growth. The methanolic extracts from treated roots were analysed by reverse phase ultra-high-performance liquid chromatography hyphenated with ion trap time-of-flight high-resolution mass spectrometry to compare their metabolites with that of control plants (B0). Results:We found, in both treated and control plants, several primary metabolites, such as fatty acids and coumaric acid, and other compounds associated with secondary metabolism pathways such as that of cyclopentaneoctanoic acid (CPOA) or hydroxyoctadecadienoic acid (HODE), and additional molecules which were not characterizable with the available data. A semiquantitative assessment of all metabolites became the basis for further processing the metabolic results by principal component analysis, which highlighted significant differences in the PC1 and PC2 components. The PC1 was particularly affected by the presence of arachidonic acid, myristic acid, and two unidentified metabolites. It effectively differentiated control plants from all bioeffectors treatments, and, in particular, the B4 treatment from the rest (B1-B3). The PC2 was mainly affected by palmitic acid, heptadecanoic acid, two CPOAs, one HODE and two unidentified metabolites. These metabolites successfully differentiated the B0 control from all the bioeffectors treatments, and, especially, showed a difference between B1 and B2. Conclusions:Our findings suggest that changes in secondary pathways of lipid metabolism may underlie the biostimulation exerted by the four microbial bioeffectors of this study, and that LC-MS coupled by multivariate analysis can easily fingerprint the metabolic alterations induced by bioeffectors in tomato roots.

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Simultaneous metabolite fingerprinting of hydrophilic and lipophilic compounds in Echinacea pallida by high-performance liquid chromatography with diode array and electrospray ionization-mass spectrometry detection

Cited by 20 publications

References 24 publications

The Medicinal Chemistry of Echinacea Species

The Medicinal Chemistry of Echinacea Species

In vitro regeneration, Agrobacterium-mediated transformation, and genetic assay of chalcone synthase in the medicinal plant Echinacea pallida

Phytochemical profiling of tomato roots following treatments with different microbial inoculants as revealed by IT-TOF mass spectrometry

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