Simultaneous measurement of serotonin, catecholamines and their metabolites in cat and human plasma by in vitro microdialysis—microbore high-performance liquid chromatography with amperometric detection

Sympathetic activity and myocardial damage after stimulation of dorsal medulla and vagotomy in a novel animal model

“…The dorsal medulla is an important central sympathetic center [19]. An electrical stimulation to this area can elicit significant hypersympathetic activity including hypertension and tachycardia [19][20][21][22][23][24]. The technique to stimulate the dorsal medulla has been well developed in the feline model [19][20][21][22][23][24].…”

“…Plasma concentration of norepinephrine was measured by high performance liquid chromatography at 0, 5, 15, 30, 60, 90, 120 and 180 min during electrical stimulation [24].…”

“…An electrical stimulation to this area elicits significant hypersympathetic activity including hypertension and tachycardia [19][20][21][22][23][24]. Taking the advantage of these animal models in producing sympathetic hyperactivity, we developed a novel feline model for correlation of the sympathetic excitation with ventricular dysfunction and myocardial damage.…”

Micellar liquid chromatography determination of some biogenic amines with electrochemical detection

“…High performance liquid chromatography (HPLC) has been reported for the analysis of BAs and their metabolites in biological fluids [4][5][6][7][8][9]. Several authors use an HPLC procedure with either amperometric [10,11] or coulometric [12] detection and a C18 column for the analysis of BAs in serum or urine samples. Other HPLC procedures applied to biological samples include precolumn BA oxidation to aminochromes with spectrometric or coulometric detection [13][14][15][16].…”

Phytoestrogen Cimicifugoside-Mediated Inhibition of Catecholamine Secretion by Blocking Nicotinic Acetylcholine Receptor in Bovine Adrenal Chromaffin Cells

“…Catecholamine secretion from chromaffin cells was measured in 24-well plates following the method previously reported (Cheng et al, 1992(Cheng et al, , 1993Eaton et al, 2000). Briefly, cells were rinsed two times with Ca 2ϩ -containing Locke's solution containing 157.4 mM NaCl, 5.6 mM KCl, 2.2 mM CaCl 2 , 1.2 mM MgCl 2 , 5.6 mM D-glucose, 5 mM HEPES, 3.6 mM NaHCO 3 , pH 7.4 titrated by NaOH and incubated at 37°C for 5 min in each case.…”