Simultaneous LC/MS/MS quantification of eight apolipoproteins in normal and hypercholesterolemic mouse plasma

Abstract

Alterations of plasma lipoproteins, especially elevated levels of apoB-containing lipoproteins, have been causally related to the risk of atherosclerotic CVD (ASCVD) (1). Apolipoproteins are major structural and functional components of lipoproteins with diverse biologic functions. They can serve as cofactors for enzymes and ligands for cell-surface receptors (2). Thereby, apolipoproteins are intimately involved in the metabolism of plasma cholesterol and triglycerides carried by lipoproteins. Blood concentrations of apoB and apoA-I, as surrogate measures for VLDL/ LDL and HDL, have been proposed as superior markers for the assessment of cardiovascular risk in several trials (3, 4). In addition, apolipoproteins like apoA-II, apoA-IV, apoC-I, apoC-II, apoC-III, and apoE that can associate with various lipoprotein classes are also key players in lipid metabolism and may represent targets for the diagnosis and treatment of dyslipidemias and ASCVD (5-7). In this context, Pechlaner et al. (8) recently reported associations of several apolipoproteins (including apoC-II, apoC-III, apoE, apoH, and apoL1) with incident CVD in a prospective population-based study, which raises the potential prospects of apolipoprotein profiling for CVD.

Development of an antibody-free ID-LC MS method for the quantification of procalcitonin in human serum at sub-microgram per liter level using a peptide-based calibration

“…This demonstrates that other material losses occurring during the sample preparation steps are significant. Thus, a correction factor is required in any case, even with a digestion time of 0.5 h. Although the peptide-based calibration approach is widely applied thanks to its ease of use and reduced cost [47][48][49][50][51][52], our results agree with a significant quantitative bias using this approach, as reported in other studies [53][54][55].…”

“…In the proposed method, the quantification of PCT in human serum was performed using a peptide-based calibration. This strategy was described to be a suitable approach for absolute quantification of low abundant protein biomarkers and for medium/high abundance proteins with complex sample preparation [50,62,63]. However, in the present study, the quantification of protein-based QC materials (recombinant protein spiked in serum) evidenced the necessity to use a correction factor to quantify PCT in serum samples accurately.…”

Apolipoprotein A4 Restricts Diet‐Induced Hepatic Steatosis via SREBF1‐Mediated Lipogenesis and Enhances IRS‐PI3K‐Akt Signaling

“…[33] The dose of ApoA4 one-time injection for each animal was 1 mg of ApoA4 kg −1 body weight, and such an amount was within the physiologic range of ApoA4 concentration in murine plasma and equivalents to human plasma ApoA4 levels. [25,34,35]…”

Simultaneous quantification of apolipoproteins A-I, E, and J in human plasma by LC-MS/MS for clinical application to diabetes mellitus complicated with cardiovascular disease

“…14 During the last years, liquid chromatography tandem mass spectrometry (LC-MS/MS) methods with multiple reaction monitoring (MRM) mode have been widely used in targeted quantitative proteomic analysis which can greatly reduce the background noise and improve the sensitivity, specificity and precision. 18–20 Compared with traditional immunological methods, MRM method shows great advantages in terms of accuracy, selectivity, reproducibility, sensitivity, and quantitative linear range, and multiple proteins can be analyzed from small sample volumes within one run. 14,18–22…”

“…18–20 Compared with traditional immunological methods, MRM method shows great advantages in terms of accuracy, selectivity, reproducibility, sensitivity, and quantitative linear range, and multiple proteins can be analyzed from small sample volumes within one run. 14,18–22…”