Simultaneous isolation of seven compounds from<i>Glehnia littoralis</i>roots by off-line overpressured layer chromatography guided by a TLC antioxidant autographic assay

Su, Xing; Li, Xiangkun; Tao, Hongxun; Zhou, Jianhua; Wu, Tao; Chou, Gui‐Xin; Cheng, Zhihong

doi:10.1002/jssc.201300523

Cited by 16 publications

(12 citation statements)

References 25 publications

(26 reference statements)

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“…The difficulties experienced to obtain a reproducible homogeneous layer of buffered enzyme suspension onto the hydrophobic surface of RP‐TLC plates by direct spray, made necessary to search for another strategy for enzyme application. Enzyme immobilization by gel entrapment has been applied for the development of xanthine oxidase , β‐glucosidase , tyrosinase , and AChE autographic assays. This strategy increases enzyme stability, decreases operational difficulties and improves reproducibility.…”

Section: Resultsmentioning

confidence: 99%

A reversed‐phase compatible thin‐layer chromatography autography for the detection of acetylcholinesterase inhibitors

Ramallo

García

Furlán

2015

J of Separation Science

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A dual readout autographic assay to detect acetylcholinesterase inhibitors present in complex matrices adsorbed on reversed-phase or normal-phase thin-layer chromatography plates is described. Enzyme gel entrapment with an amphiphilic copolymer was used for assay development. The effects of substrate and enzyme concentrations, pH, incubation time, and incubation temperature on the sensitivity and the detection limit of the assay were evaluated. Experimental design and response surface methodology were used to optimize conditions with a minimum number of experiments. The assay allowed the detection of 0.01% w/w of physostigmine in both a spiked Sonchus oleraceus L. extract chromatographed on normal phase and a spiked Pimenta racemosa (Mill.) J.W. Moore leaf essential oil chromatographed on reversed phase. Finally, the reversed-phase thin-layer chromatography assay was applied to reveal the presence of an inhibitor in the Cymbopogon citratus (DC.) Stapf essential oil. The developed assay is able to detect acetylcholinesterase inhibitors present in complex matrixes that were chromatographed in normal phase or reversed-phase thin-layer chromatography. The detection limit for physostigmine on both normal and reversed phase was of 1×10(-4) μg. The results can be read by a change in color and/or a change in fluorescence.

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Section: Resultsmentioning

confidence: 99%

A reversed‐phase compatible thin‐layer chromatography autography for the detection of acetylcholinesterase inhibitors

Ramallo

García

Furlán

2015

J of Separation Science

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“…Dried root, dried underground parts [39,42] 2-Methoxy-4-(1-propionyl) phenyl β-D-glucopyranoside (8) Fresh root and rhizome [43] Syringin 9Dried root, dried roots bark [41,44] Glehnilate 10Dried underground parts [45] Evidence-Based Complementary and Alternative Medicine Dried roots, dried roots and rhizomes [46][47][48] Esculetin (12) Dried root [49] Umbelliferone 7-O-β-D-glucopyranoside (13) Fresh fruit, dried root [38,50] Scopoletin 14Dried root and rhizome, dried root, dried underground parts [38, 39, 46-48, 51, 52] Scopolin (15) Dried root [38,49] Scopoletin-apiosylglucoside (16) Dried root [38] Osthenol 7-O-β-D-gentibioside (17) Fresh fruit [50] 6-(3,3-Dimethylallyl)-7-hydroxycoumarin (18) Dried root [38] 6-(3,3-Dimethylallyl)-7-hydroxycoumarin-apiosyl-glucoside (19) Dried root [38] 6-(3,3-Dimethylallyl)-7-hydroxycoumarindiglucoside (20) Dried root [38] 7-O-(3,3-Dimethylallyl) scopoletin (21) Dried root and rhizome [52] (S)-Peucedanol 7-O-β-D-glucopyranoside (22)…”

Section: Coumarinsmentioning

confidence: 99%

Ethnopharmacology, Phytochemistry, and Pharmacology of the GenusGlehnia: A Systematic Review

Yang

Zhang

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Glehnia littoralis Fr. Schmidt ex Miq, the sole species in the genus Glehnia (Apiaceae), has long been used in traditional Chinese medicine to treat fatigue, weakness, stomach-yin deficiency, lung heat, cough, dry throat, and thirst. Recently, G. littoralis has also been incorporated into a wide range of Chinese vegetarian cuisines. Based on the comprehensive information, advances in botany, known uses, phytochemistry, pharmacology, and toxicity of G. littoralis, we aim to highlight research gaps and challenges in studying G. littoralis as well as to explore its potential use in plant biotechnology. This may provide more efficient therapeutic agents and health products from G. littoralis. A literature search of SciFinder, ScienceDirect, Scopus, TPL, Google Scholar, Baidu Scholar, and Web of Science, books, PhD and MSc dissertations, and peer-reviewed papers on G. littoralis research was conducted and comprehensively analyzed. We confirmed that the ethnomedical uses of G. littoralis have been recorded in China, Japan, and Korea for thousands of years. A phytochemical investigation revealed that the primary active compounds were phenylpropanoids, coumarins, lignanoids, and flavonoids, organic acids and derivatives, terpenoids, polyacetylenes, steroids, nitrogen compounds, and others. Our analysis also confirmed that the extracts of G. littoralis possess immunoregulatory, antitumor, anti-inflammatory, hepatoprotective, antioxidant, neuroprotective, antibacterial, antifungal, and analgesic properties. Although further studies are required, there is strong evidence of the antitumor and immunoregulatory potential of G. littoralis. Also, more studies are needed to elucidate the mechanisms of action of its active compounds (e.g., falcarinol and panaxydiol) before any clinical studies can be carried out.

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“…• Anti-cholinesterase compounds using either the Ellman reagent or the fast blue salt B 28,29 • α-and β-Glucosidase inhibitors 30,31 • Xanthine oxidase inhibitors 32,33 • …”

Section: 20mentioning

confidence: 99%

Bioassay-coupled Chromatographic Analysis of Medicinal Natural Products: A Review

Elufioye¹

2017

TJNPR

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Simultaneous isolation of seven compounds fromGlehnia littoralisroots by off-line overpressured layer chromatography guided by a TLC antioxidant autographic assay

Cited by 16 publications

References 25 publications

A reversed‐phase compatible thin‐layer chromatography autography for the detection of acetylcholinesterase inhibitors

A reversed‐phase compatible thin‐layer chromatography autography for the detection of acetylcholinesterase inhibitors

Ethnopharmacology, Phytochemistry, and Pharmacology of the GenusGlehnia: A Systematic Review

Bioassay-coupled Chromatographic Analysis of Medicinal Natural Products: A Review

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