Simultaneous ion chromatographic separation of anions and cations on poly(aspartic acid) functionalized silica

Kiseleva, Maria; Kebets, P. A.; Nesterenko, Pavel N.

doi:10.1039/b106283m

Cited by 26 publications

(15 citation statements)

References 14 publications

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“…Polyaspartic acid-bonded silica has been developed and commercialized as PolyCAT A. It has been used as zwitterionic phase for simultaneous separation of inorganic anions and cations, , due to the presence of both the unreacted protonated propylamine and the dissociated carboxyl functionality of the polyaspartic acid attached to the silica. PolyCAT A has also been used as a weak cation-exchange material for protein separation and in the ERLIC mode for removal of SDS from a peptide digest before fractionation of the peptides .…”

Section: Results and Discussionmentioning

confidence: 99%

Profiling of Human Milk Oligosaccharides for Lewis Epitopes and Secretor Status by Electrostatic Repulsion Hydrophilic Interaction Chromatography Coupled with Negative-Ion Electrospray Tandem Mass Spectrometry

et al. 2019

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Human milk oligosaccharides (HMOs) are one of the most abundant ingredients in breast milk, and they play a beneficial role for newborns and are important for infant health. The peripheral fucosylated sequences of HMOs, such as the histo-blood group ABH(O) and Lewis a, b, x, and y antigens, are determined by the expression of the secretor (Se) and Lewis (Le) genes in the mammary gland, and are often the recognition motifs and serve as decoy receptors for microbes. In this work, we developed a method for determination of secretor status and Lewis blood phenotype and assignment of Lewis blood-group epitopes. The method was based on electrostatic repulsion/hydrophilic interaction chromatography coupled with tandem mass spectrometry (ERLIC–MS/MS). A specifically designed stationary phase, aspartic acid-bonded silica (ABS), was used to separate the acidic and neutral HMOs by electrostatic repulsion followed by HILIC. Negative-ion electrospray MS/MS was then used for analysis of secretor status and Lewis blood phenotypes and assignment of important epitopes of HMOs from the lactating mothers by selecting a specific set of unique fragment ions.

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Section: Results and Discussionmentioning

confidence: 99%

Profiling of Human Milk Oligosaccharides for Lewis Epitopes and Secretor Status by Electrostatic Repulsion Hydrophilic Interaction Chromatography Coupled with Negative-Ion Electrospray Tandem Mass Spectrometry

et al. 2019

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“…Poly(aspartic acid)‐bonded silica is an interesting material that exhibits zwitterionic character . Depending on the eluent pH, anion, or cation exchange is dominant.…”

Section: Peptide‐bonded Stationary Phasesmentioning

confidence: 99%

“…Depending on the eluent pH, anion, or cation exchange is dominant. On this stationary phase, the simultaneous separation of cations and anions is possible in the pH range of 3.0–3.5 . The structure of the material is shown in Fig.…”

Section: Peptide‐bonded Stationary Phasesmentioning

confidence: 99%

Amino acids, peptides, and proteins as chemically bonded stationary phases – A review

Bocian

Skoczylas

Buszewski

2015

J of Separation Science

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The selectivity of chromatographic separation depends mostly on the stationary phase and mobile phase composition. Despite being a material with bonded simple organic molecule, a wide group of stationary phases contain immobilized compound that possesses biological activity. Stationary phases that contain amino acids and peptides as well as enzymes and proteins are alternative materials that may be used for liquid chromatographic separations and are reviewed in this work. In the case of peptide-bonded stationary phases, most of these types of materials were elaborated in the 1970s and 1980s; however, over the last few years a growing interest has been observed which is connected with hydrophilic interaction liquid chromatography. The most important application of amino acid and peptide-bonded stationary phases is connected with separation of amino acids, their derivatives, and peptides. The main advantage of such materials is the ability for chiral separations.

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“…Organofunctionalized silica materials have received special attention for many years due to the large number of potential applications, especially in separation science as adsorbents for solid phase extraction (SPE), stationary phase for chromatography or metal adsorbent matrices. [1][2][3][4] The use of several chelating organofunctional groups grafted onto a silica gel surface has been described. 5,6 However, the adsorbents prepared in this way rarely surpass 0.3 mmol g 21 , which is small if compared with organic resins.…”

Section: Introductionmentioning

confidence: 99%

4-Phenylenediaminepropylsilica xerogel as a sorbent for copper determination in waters by slurry-sampling ETAAS

Vaghetti

Zat

Bentes

et al. 2003

J. Anal. At. Spectrom.

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The synthesis of a new, hybrid mesoporous sorbent, 4-phenylenediaminepropylsilica (4-PhAP/silica xerogel) obtained by the sol-gel process is described. This material was characterized by infrared spectroscopy (IR), elemental analysis (CHN), and nitrogen adsorption-desorption isotherms. Subsequently, this material was employed for the successful pre-concentration of copper in natural water reference materials. The copper characteristic mass was 15.0 ¡ 0.2 pg. The detection limit for copper determination in natural waters employing the pre-concentration procedure was 0.2 mg l 21 . The average tube lifetime was 750 analytical firings when 20 ml of 1.5% m/v copper sorbed on 4-PhAP/silica xerogel slurry was delivered to a previously treated W-Rh treated platform of a transversely heated graphite atomizer (TGA). The pre-concentration factor obtained with 4-PhAP/silica xerogel as adsorbent for copper determination was 3.75 and the retention capacity of the sorbent was 0.52 mmol copper per gram of sorbent.

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Simultaneous ion chromatographic separation of anions and cations on poly(aspartic acid) functionalized silica

Cited by 26 publications

References 14 publications

Profiling of Human Milk Oligosaccharides for Lewis Epitopes and Secretor Status by Electrostatic Repulsion Hydrophilic Interaction Chromatography Coupled with Negative-Ion Electrospray Tandem Mass Spectrometry

Profiling of Human Milk Oligosaccharides for Lewis Epitopes and Secretor Status by Electrostatic Repulsion Hydrophilic Interaction Chromatography Coupled with Negative-Ion Electrospray Tandem Mass Spectrometry

Amino acids, peptides, and proteins as chemically bonded stationary phases – A review

4-Phenylenediaminepropylsilica xerogel as a sorbent for copper determination in waters by slurry-sampling ETAAS

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