Simultaneous induction of biomass and lipid production in Tetradesmus obliquus BPL16 through polysorbate supplementation

Esakkimuthu, Sivakumar; Krishnamurthy, Venkatesan; Wang, Shuang; Abomohra, Abd El‐Fatah; Ramakrishnan, Sankar Ganesh; Subrmaniam, Sadhasivam; Swaminathan, K.

doi:10.1016/j.renene.2019.03.104

Cited by 21 publications

(5 citation statements)

References 56 publications

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“…When 0.2 % polysorbates were added, the growth of the microalgae completely retorted. This polysorbate inhibitory effect is described by the mechanism of interfering with cellular membrane porosity and therefore, leading to cell damage [34]. In the current study, polysorbate Tween 20, 40 and 80 did not inhibit R. glutinis growth at concentrations up to 10 %.…”

Section: Discussioncontrasting

confidence: 53%

“…Thirdly, according to the literature, polysorbate at optimal concentration increases the permeability of microorganism cells, expediting the transport of matters through the cell membranes, resulting in the increase in substrate entry effectiveness and enzyme secretion. On the other hand, the effect of polysorbates on the permeability of the cell membrane of a microorganism depends on the concentration and can have both positive and negative effects [30], [34]. Esakkimuthu et al [34] study shows that the addition of 0.1% polysorbates Tween 20, 40, and 80 to the medium increases the growth of the biomass of microalgae T. obliquus; with an increase in concentration to 0.15 %, the weight of the biomass decreased.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, the effect of polysorbates on the permeability of the cell membrane of a microorganism depends on the concentration and can have both positive and negative effects [30], [34]. Esakkimuthu et al [34] study shows that the addition of 0.1% polysorbates Tween 20, 40, and 80 to the medium increases the growth of the biomass of microalgae T. obliquus; with an increase in concentration to 0.15 %, the weight of the biomass decreased. When 0.2 % polysorbates were added, the growth of the microalgae completely retorted.…”

Section: Discussionmentioning

confidence: 99%

“…Secondly, possible reason for the increase in biomass weight may be associated with the ability of some microorganisms to use polysorbates as a carbon source. It was found that some microorganisms as microalgae Tetradesmus obliquus [34] and Lactobacillus plantarum [30] can utilize the fatty acid moieties of polysorbates successfully in the biosynthetic pathways. In the current study, the addition of the 10 % Tween 20, 40 and 80 showed the highest results and have increased biomass weight by 186 %, 126 % and 147 % respectively, compared to the control (Fig.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Polysorbates on the Growth of Rhodotorula Glutinis in Oil Rich Medium

Raita

Spalviņš

Raits

et al. 2021

Environmental and Climate Technologies

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The present study has investigated the effect of oil rich medium supplementation with polysorbates Tween 20, 40 and 80, for the cultivation of red oleaginous yeast Rhodotorula glutinis. R. glutinis has been cultivated in yeast extract peptone glucose modified broth (mYPG) supplemented with 2 % of waste cooking rapeseed oil and three polysorbate types with 0.5 %, 1 %, 2%, 3 %, 4 %, 5 %, 6 %, 7 %, 8 %, 9 % and 10 % concentration each. Yeast biomass was measured by the thermogravimetric method at 105 °C each day during 7-day experiment. The oil rich medium supplementation with Tween 20, 40 and 80 at concentrations ranging from 2 % to 10 % significantly increased the biomass of R. glutinis. All three types of the studied polysorbates with 0.5 % and 1 % concentration, did not affect yeast growth and the dry biomass – results were similar to the control sample without polysorbate addition. Between the three types of polysorbates, Tween 20 was selected as the preferable for R. glutinis cultivation with an optimal concentration of 2 %. Cultivation of R. glutinis in oil rich medium with polysorbates Tween 20, 40 and 80, supplementation up to 10 % concentration did not have had an inhibitory effect on the biomass growth.

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Section: Discussioncontrasting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effect of Polysorbates on the Growth of Rhodotorula Glutinis in Oil Rich Medium

Raita

Spalviņš

Raits

et al. 2021

Environmental and Climate Technologies

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“…However, nutrient starvation leads to a significant reduction in biomass production, markedly reducing microalgae’s lipid productivity. Additionally, genetic engineering requires extensive knowledge about the compartmentalization of photosynthesis, TAG synthesis and regulation, and the connection between TAG synthesis ( Esakkimuthu et al, 2019 ).…”

Section: Microalgae Fungi Co-cultivationmentioning

confidence: 99%

Microalgal co-cultivation -recent methods, trends in omic-studies, applications, and future challenges

Naseema Rasheed,

Pourbakhtiar,

Mehdizadeh Allaf

et al. 2023

Front. Bioeng. Biotechnol.

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The burgeoning human population has resulted in an augmented demand for raw materials and energy sources, which in turn has led to a deleterious environmental impact marked by elevated greenhouse gas (GHG) emissions, acidification of water bodies, and escalating global temperatures. Therefore, it is imperative that modern society develop sustainable technologies to avert future environmental degradation and generate alternative bioproduct-producing technologies. A promising approach to tackling this challenge involves utilizing natural microbial consortia or designing synthetic communities of microorganisms as a foundation to develop diverse and sustainable applications for bioproduct production, wastewater treatment, GHG emission reduction, energy crisis alleviation, and soil fertility enhancement. Microalgae, which are photosynthetic microorganisms that inhabit aquatic environments and exhibit a high capacity for CO2 fixation, are particularly appealing in this context. They can convert light energy and atmospheric CO2 or industrial flue gases into valuable biomass and organic chemicals, thereby contributing to GHG emission reduction. To date, most microalgae cultivation studies have focused on monoculture systems. However, maintaining a microalgae monoculture system can be challenging due to contamination by other microorganisms (e.g., yeasts, fungi, bacteria, and other microalgae species), which can lead to low productivity, culture collapse, and low-quality biomass. Co-culture systems, which produce robust microorganism consortia or communities, present a compelling strategy for addressing contamination problems. In recent years, research and development of innovative co-cultivation techniques have substantially increased. Nevertheless, many microalgae co-culturing technologies remain in the developmental phase and have yet to be scaled and commercialized. Accordingly, this review presents a thorough literature review of research conducted in the last few decades, exploring the advantages and disadvantages of microalgae co-cultivation systems that involve microalgae-bacteria, microalgae-fungi, and microalgae-microalgae/algae systems. The manuscript also addresses diverse uses of co-culture systems, and growing methods, and includes one of the most exciting research areas in co-culturing systems, which are omic studies that elucidate different interaction mechanisms among microbial communities. Finally, the manuscript discusses the economic viability, future challenges, and prospects of microalgal co-cultivation methods.

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