Simultaneous in vivo monitoring of hepatic glucose and glucose-6-phosphate by13C-NMR spectroscopy

Combination of two fat saturation pulses improves detectability of glucose signals in carbon-13 MR spectroscopy

“…Signal assignments of the oil were based on references. 12–15) The reduction rate of 7 out of 8 peaks with both suppression pulses showed good agreement with the sum of separate SAT/CHESS pulses. With the combination of SAT and CHESS pulses, the residual rate of the oil carbonyl carbon peak (C=O, 168 ppm) was 40% of that without fat suppression (60% reduction), however, it was 83% (only 17% reduction) with SAT alone.…”

“…The baseline (100%) was obtained by using the highest peak induced by J couplings without the two fat suppression pulse sequences. The chemical shifts (ppm) were observed in this experiment and their assignments were based on the references 12,13,14,15) (see Fig. 2).…”

Evidence Supporting a cis-enediol-based Mechanism for Pyrococcus furiosus Phosphoglucose Isomerase

“…1 H and 13 C resonances for G6P and F6P were assigned by comparison with literature values. 44,45 To assist in the assignment of the products of the above reactions, similar spectra were accumulated on a sample of 23 mg/ml phosphomannose isomerase in 10 mM M6P in 2 H 2 O. In addition, 1 H 1D spectra were run after addition of 0.13 mg/ml of PfPGI to both F6P and G6P to observe the growth of the anomeric protons of G6P and the H1 resonance of F6P, respectively.…”

13C magnetic resonance spectroscopy detection of changes in serine isotopomers reflects changes in mitochondrial redox status

“…The extra concern of this method is the peak separation of the serine isotopomers in the in vivo spectra. The carbon–carbon one bond coupling constant is about 37 Hz in for 2,3‐ 13 C‐serine, which is about 0.5 ppm at 7 T and 2.3 ppm at 1.5 T. It has been shown that it is possible to obtain excellent peak quantification results even from peaks only with 0.14 ppm separation from the rat liver at 7 T, using routine deconvolution of overlapping peaks (49). Therefore, it is reasonable to assume that the relative peak ratio quantification in this study would be enough to produce conclusive results when 13 C glycine is used in in vivo human and animal MRS experiments.…”