Simultaneous immunoassays of dual prostate cancer markers using a SERS-based microdroplet channel

Gao, Rongke; Cheng, Ziyi; Wang, Xiaokun; Yu, Liandong; Guo, Zhongyi; Zhao, Gang; Choo, Jaebum

doi:10.1016/j.bios.2018.08.015

Cited by 88 publications

(47 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The replacement of microchannel rather than conventional microwell plate provides additional advantages to this multistep analysis technique, including rapid, automatic, portable, as well as low sample and solvent consumption. Choo's group contributed a series of good examples to automatic multistep microfluidic SERS immunoassay [54,[112][113][114]. In their build-in microfluidic SERS system, sample solution, wash buffer, polyclonal antibody-conjugated SERS agent solution can fully automatically pass through the immunoassay and SERS detection zone [54].…”

Section: Multistep Biomacromolecule and Cell Surface-enhanced Raman Spectroscopy Analysismentioning

confidence: 99%

Recent progress of microfluidics in surface‐enhanced Raman spectroscopic analysis

Xia

2021

J of Separation Science

View full text Add to dashboard Cite

Surface-enhanced Raman spectroscopy is a significant analytical tool capable of fingerprint identification of molecule in a rapid and ultrasensitive manner. However, it is still hard to meet the requirements of practical sample analysis.The introduction of microfluidics can effectively enhance the performance of surface-enhanced Raman spectroscopy in complex sample analysis including reproducibility, selectivity, sensitivity, and speed. This review summarizes the recent progress of microfluidics in surface-enhanced Raman spectroscopic analysis through four combination approaches. First, microfluidic synthetic techniques offer uniform nano-/microparticle fabrication approaches for reproductive surface-enhanced Raman spectroscopic analysis. Second, the integration of microchip and surface-enhanced Raman spectroscopic substrate provides advanced devices for sensitive and efficient detection. Third, microfluidic sample preparations enable rapid separation and preconcentration of analyte prior to surface-enhanced Raman spectroscopic detection. Fourth, highly integrated microfluidic devices can be employed to realize multistep surface-enhanced Raman spectroscopic analysis containing material fabrication, sample preparation, and detection processes. Furthermore, the challenges and outlooks of the application of microfluidics in surface-enhanced Raman spectroscopic analysis are discussed.

show abstract

Section: Multistep Biomacromolecule and Cell Surface-enhanced Raman Spectroscopy Analysismentioning

confidence: 99%

Recent progress of microfluidics in surface‐enhanced Raman spectroscopic analysis

Xia

2021

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Prostate cancer is fifth cause of cancer death in the world and the second abundant cancer among men [ 117 , 118 , 119 ]. Currently, imaging techniques, MRI, ultrasonography, digital rectal examination (DRE), computed tomography (CT), and cancer protein assay are various clinical diagnostic strategies for PCa detection [ 120 , 121 ]. While all of these methods are powerful and highly successful in detecting of PCa, most of these approaches still complain about lack of precision, sensitivity and specificity for clinical purposes [ 120 , 122 ].…”

Section: Nanomaterials Towards Diagnosis and Biosensing Of Prostatmentioning

confidence: 99%

“…Currently, imaging techniques, MRI, ultrasonography, digital rectal examination (DRE), computed tomography (CT), and cancer protein assay are various clinical diagnostic strategies for PCa detection [ 120 , 121 ]. While all of these methods are powerful and highly successful in detecting of PCa, most of these approaches still complain about lack of precision, sensitivity and specificity for clinical purposes [ 120 , 122 ]. Evidence strongly indicate that the tracking of PCa at the first development stage can help maximize the effectiveness for medical approaches and improve cancer survival from 10% to 90% [ 120 ].…”

Section: Nanomaterials Towards Diagnosis and Biosensing Of Prostatmentioning

confidence: 99%

“…While all of these methods are powerful and highly successful in detecting of PCa, most of these approaches still complain about lack of precision, sensitivity and specificity for clinical purposes [ 120 , 122 ]. Evidence strongly indicate that the tracking of PCa at the first development stage can help maximize the effectiveness for medical approaches and improve cancer survival from 10% to 90% [ 120 ]. Nano-medicine can represent new interventions for the early detection of cancer based on PCa biomarkers, and resultantly, many attempts have been undertaken to develop novel nanotechnology-based diagnostics for early detection of cancers [ 71 , 123 , 124 , 125 ].…”

Section: Nanomaterials Towards Diagnosis and Biosensing Of Prostatmentioning

confidence: 99%

“…Simultaneous identification of multiple biomarkers plays a significant role in accurate and consistent cancer diagnosis. In a study, the Gao group also developed microfluidic devices based on surface-enhanced Raman scattering (SERS) for the simultaneous detection of total PSA (t-PSA) and free PSA (f-PSA) biomarkers [ 120 ]. Their data showed very good linear response from 0.05 to 100 ng/mL for both PSA markers.…”

Section: Nanomaterials Towards Diagnosis and Biosensing Of Prostatmentioning

confidence: 99%

See 2 more Smart Citations

Nanotreatment and Nanodiagnosis of Prostate Cancer: Recent Updates

et al. 2020

View full text Add to dashboard Cite

The fabrication and development of nanomaterials for the treatment of prostate cancer have gained significant appraisal in recent years. Advancements in synthesis of organic and inorganic nanomaterials with charge, particle size, specified geometry, ligand attachment etc have resulted in greater biocompatibility and active targeting at cancer site. Despite all of the advances made over the years in discovering drugs, methods, and new biomarkers for cancer of the prostate (PCa), PCa remains one of the most troubling cancers among people. Early on, effective diagnosis is an essential part of treating prostate cancer. Prostate-specific antigen (PSA) or serum prostate-specific antigen is the best serum marker widely accessible for diagnosis of PCa. Numerous efforts have been made over the past decade to design new biosensor-based strategies for biomolecules detection and PSA miniaturization biomarkers. The growing nanotechnology is expected to have a significant effect in the immediate future on scientific research and healthcare. Nanotechnology is thus predicted to find a way to solve one of the most and long-standing problem, “early cancer detection”. For early diagnosis of PCa biomarkers, different nanoparticles with different approaches have been used. In this review, we provide a brief description of the latest achievements and advances in the use of nanoparticles for PCa biomarker diagnosis.

show abstract

Digital droplet immunoassay based on a microfluidic chip with magnetic beads for the detection of prostate‐specific antigen

Zeng,

Tian,

Ren

et al. 2023

J of Separation Science

View full text Add to dashboard Cite

Sensitive biomarker detection techniques are beneficial for both disease diagnosis and postoperative examinations. In this study, we report an integrated microfluidic chip designed for the immunodetection of prostate‐specific antigens (PSAs). The microfluidic chip is based on the three‐dimensional structure of quartz capillaries. The outlet channel extends to 1.8 cm, effectively facilitating the generation of uniform droplets ranging in size from 3 to 50 μm. Furthermore, we successfully immobilized the captured antibodies onto the surface of magnetic beads using an activator, and we constructed an immunosandwich complex by employing biotinylated antibodies. A key feature of this microfluidic chip is its integration of microfluidic droplet technology advantages, such as high‐throughput parallelism, enzymatic signal amplification, and small droplet size. This integration results in an exceptionally sensitive PSA detection capability, with the detection limit reduced to 7.00 ± 0.62 pg/mL.

show abstract

Simultaneous immunoassays of dual prostate cancer markers using a SERS-based microdroplet channel

Cited by 88 publications

References 41 publications

Recent progress of microfluidics in surface‐enhanced Raman spectroscopic analysis

Recent progress of microfluidics in surface‐enhanced Raman spectroscopic analysis

Nanotreatment and Nanodiagnosis of Prostate Cancer: Recent Updates

Digital droplet immunoassay based on a microfluidic chip with magnetic beads for the detection of prostate‐specific antigen

Contact Info

Product

Resources

About