Simultaneous Imaging of Three Tumor-Related mRNAs in Living Cells with a DNA Tetrahedron-Based Multicolor Nanoprobe

Wang, Song; Xia, Meng-Chan; Liu, Jie; Zhang, Sichun; Zhang, Xinrong

doi:10.1021/acssensors.7b00290

Cited by 63 publications

(46 citation statements)

References 40 publications

(32 reference statements)

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“…Their strategy proved efficacious for imaging miR‐155 and miR‐21 simultaneously in MDA‐MB‐231 cells. Wang et al expanded capabilities to simultaneous detection of three different mRNA targets . c‐Myc, TK1, and GalNAc‐T mRNA were simultaneously studied in MCF‐7 and MCF‐10a cells.…”

Section: Hybridization‐based Probesmentioning

confidence: 99%

Nucleic‐Acid Structures as Intracellular Probes for Live Cells

2019

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The chemical composition of cells at the molecular level determines their growth, differentiation, structure, and function. Probing this composition is powerful because it provides invaluable insight into chemical processes inside cells and in certain cases allows disease diagnosis based on molecular profiles. However, many techniques analyze fixed cells or lysates of bulk populations, in which information about dynamics and cellular heterogeneity is lost. Recently, nucleic‐acid‐based probes have emerged as a promising platform for the detection of a wide variety of intracellular analytes in live cells with single‐cell resolution. Recent advances in this field are described and common strategies for probe design, types of targets that can be identified, current limitations, and future directions are discussed.

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Section: Hybridization‐based Probesmentioning

confidence: 99%

Nucleic‐Acid Structures as Intracellular Probes for Live Cells

2019

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“…FNA‐based fluorescent biosensors can be used for multiplex detection ,. For instance, Pei group proposed a DNA nanostructure‐supported aptamer pull‐down (DNaPull) assay for single‐cell analysis (Figure b) .…”

Section: Fna‐based Biosensorsmentioning

confidence: 99%

Rational Design of Framework Nucleic Acids for Bioanalytical Applications

Liu

et al. 2019

ChemPlusChem

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With the advent of DNA nanotechnology, nucleic acids have been used building blocks for constructing various DNA nanostructures. As classical and simple DNA nanostructures, framework nucleic acids (FNAs) have attracted enormous attention in the field of biosensing. The sequence‐specific self‐assembly properties and high programmability of nucleic acids allow FNAs to be incorporated in advanced probe design. In addition, FNAs enable the engineering of surfaces for biosensing (e. g., probe orientation, probe spacing), thereby improving the accessibility of target molecules to the probes arranged on heterogeneous surfaces. Moreover, FNAs offer a universal and promising platform for sensing cellular molecules because of their prominent biocompatibility and cellular permeability. Herein recent advances in FNA‐based biosensors are summarized, including electrochemical detection, optical detection, and intracellular sensing. It is hoped that this review will provide guidelines for the design and construction of FNA scaffold‐based biosensors.

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“…To achieve multi‐targeting, Wang et al. constructed a tetrahedron structure with multiple overhangs, which detached and generated the corresponding fluorescence after hybridization with its target mRNA . As well as the tetrahedron structure, other distinct origami shapes (e.g., tubular) have also been experimented with for intracellular tracking …”

Section: Nucleic‐acid‐based Sensors For Functional Gene Monitoringmentioning

confidence: 99%

“…[57] To achievem ulti-targeting, Wang et al constructed at etrahedron structure with multiple overhangs, which detached andg enerated the correspondingf luorescence after hybridization with its target mRNA. [58] As well as the tetrahedron structure, other distinct origami shapes (e.g., tubular) have also been experimented with for intracellular tracking. [59] With regard to achieving intracellular mRNA monitoring, our group set out to develop af acile yet highly versatile NA sensor that would allow ap rolonged monitoring window, beyond a few days.…”

Section: In Vitro Cellular Applicationmentioning

confidence: 99%

Functional Imaging with Nucleic‐Acid‐Based Sensors: Technology, Application and Future Healthcare Prospects

et al. 2018

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Timely monitoring and assessment of human health plays a crucial role in maintaining the wellbeing of our advancing society. In addition to medical tools and devices, suitable probe agents are crucial to assist such monitoring, either in passive or active ways (i.e., sensors) through inducible signals. In this review we highlight recent developments in activatable optical sensors based on nucleic acids. Sensing mechanisms and bio-applications of these nucleic acid sensors in ex vivo assays, intracellular or in vivo settings are described. In addition, we discuss the limitations of these sensors and how nanotechnology can complement/enhance sensor properties to promote translation into clinical applications.

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Simultaneous Imaging of Three Tumor-Related mRNAs in Living Cells with a DNA Tetrahedron-Based Multicolor Nanoprobe

Cited by 63 publications

References 40 publications

Nucleic‐Acid Structures as Intracellular Probes for Live Cells

Nucleic‐Acid Structures as Intracellular Probes for Live Cells

Rational Design of Framework Nucleic Acids for Bioanalytical Applications

Functional Imaging with Nucleic‐Acid‐Based Sensors: Technology, Application and Future Healthcare Prospects

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