Simultaneous IgM reactivity by EIA against more than one virus in measles, parvovirus B19 and rubella infection

Thomas, H. I. J.; Barrett, E.; Hesketh, Louise; Wynne, Alyssa; Morgan‐Capner, P.

doi:10.1016/s1386-6532(99)00051-7

Cited by 89 publications

(61 citation statements)

References 29 publications

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“…It is our view that specificity of detection with regard to exposure to parvovirus B19 is more important than sensitivity of detection given the somewhat non-specific and sometimes confusing symptoms associated with parvovirus B19 infection (Jensen and Vestergaard, 1997). Indeed many other viral infections, particularly rubella, induce symptoms similar to parvovirus B19 (Thomas et al, 1999;Turner, 1999). However, for optimal assessment of the likelihood of infection and determination of the risk of fetal hydrops in pregnancy, serological analysis should also determine the parvovirus B19 IgG status of the patient and follow up specimens should be drawn from the patient, where possible (Searle et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Detection of parvovirus B19 IgM by antibody capture enzyme immunoassay: receiver operating characteristic analysis

Doyle

Kerr

O’Keeffe

et al. 2000

Journal of Virological Methods

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Parvovirus B19 infection can cause severe effects in high-risk groups including pregnant women and immunocompromised individuals. Although serological detection of B19 infection is commonplace, minimal information is available on the absolute performance characteristics of various tests for the detection of B19 IgM. The performance of the first parvovirus B19 IgM enzyme immunoassay to be cleared by the US Food and Drug Administration (FDA) is described. The immunoassay cut-off has been established using receiver operating characteristic (ROC) analysis giving a sensitivity and specificity of detection of 89.1 and 99.4%, respectively. No cross-reactivity is observed with rubella or other viral disease IgM which cause similar symptomologies to parvovirus B19. Multi-site reproducibility studies have shown high immunoassay reproducibility with detection rates (observed/expected result) of 100% for nonreactive specimens (N=324) and strongly reactive (N=403), respectively. Immunoassay reproducibility ranged from 11.76 to 17.46% coefficient of variation for all reactive specimens tested (N= 12) whereby each specimen was assayed a total of 81 times. Parvovirus B19 IgM seroprevalence of 1% was observed in a US blood donor population (N= 399). In the absence of international performance criteria, this study will be of major benefit to the clinical virologist in assessing immunoassay reliability for the detection of recent infection with parvovirus B19.

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Section: Discussionmentioning

confidence: 99%

Detection of parvovirus B19 IgM by antibody capture enzyme immunoassay: receiver operating characteristic analysis

Doyle

Kerr

O’Keeffe

et al. 2000

Journal of Virological Methods

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“…Serological tests such as the hemagglutination inhibition method are performed principally with paired sera from the acute and convalescent phases (5). Although IgM EIA is employed for diagnosis using a single serum sample, false-positive results of serum rubella IgM tests may occur in people with human parvovirus B19 infection, with a positive heterophile test for infectious mononucleosis, or with positive rheumatoid factor (1,24,25,28,29).…”

mentioning

confidence: 99%

Development of a New Method for Diagnosis of Rubella Virus Infection by Reverse Transcription-Loop-Mediated Isothermal Amplification

et al. 2006

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“…Thus, our negative panel of serum samples should be improved by adding samples from infected patients with herpesvirus 6 and parvovirus B19, which seem to cause some cross-reactivity with measles virus (5,25).…”

Section: Resultsmentioning

confidence: 99%

Measles serodiagnosis: production and evaluation of the IgM-measles ELISA IAL reagent

Hoshino‐Shimizu

Vaz-de-Lima

Oliveira

et al. 2001

Braz. J. Microbiol.

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Recent measles outbreaks in different countries led to an increase of laboratory measles diagnosis. Thus, we developed the IgM-Measles ELISA IAL , using measles virus antigens obtained from cell cultured in microcarriers in order to supply reagent kits to Brazilian public health laboratories. A batch of antigenic reagent was produced and evaluated in the enzyme immunoassay in comparison with clinical diagnosis and with as reference assay (IgM Capture ELISA CDC ) data. This study was performed in a positive panel with 70 serum samples from patients with measles, and a negative panel with 132 samples from patients with unrelated diseases and without recent measles or vaccination history. In relation to other diagnostic methods, the IgM ELISA IAL presented sensitivity higher than 97.1%, specificity and precision of 97%, and agreement kappa (k) index higher than 0.94 (P < 0.05). Moreover, the IgM antibody profile from measles acute phase revealed by the assay was similar to the reference assay. A practical analysis system for checking the quality of new reagent batches was proposed based on the diagnostic features and agreement kappa index. Our findings suggest that measles antigenic reagents can be produced with reliable quality control system, and supplied to public health laboratories for routine serodiagnosis or population surveys.

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Simultaneous IgM reactivity by EIA against more than one virus in measles, parvovirus B19 and rubella infection

Cited by 89 publications

References 29 publications

Detection of parvovirus B19 IgM by antibody capture enzyme immunoassay: receiver operating characteristic analysis

Detection of parvovirus B19 IgM by antibody capture enzyme immunoassay: receiver operating characteristic analysis

Development of a New Method for Diagnosis of Rubella Virus Infection by Reverse Transcription-Loop-Mediated Isothermal Amplification

Measles serodiagnosis: production and evaluation of the IgM-measles ELISA IAL reagent

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