Simultaneous identification and quantification of polymethoxyflavones, coumarin and phenolic acids in Ageratum conyzoides by UPLC-ESI-QToF-MS and UPLC-PDA

Faqueti, Larissa; Sandjo, Louis P.; Biavatti, Maique W.

doi:10.1016/j.jpba.2017.07.034

Cited by 24 publications

(22 citation statements)

References 29 publications

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“…The 1 H-NMR spectrum of the hydroalcoholic extracts of the A. conyzoides species showed signals in the region of 6-7 ppm indicative of α-oxygenated aromatic hydrogens. Also, aromatic hydrogens were observed around 8.0 ppm, possibly of peri-carbonylic hydrogens and, also singlets around 9.0 which may be related to the presence of nitrogen from aromatic alkaloids, which may be related to the presence of toxic alkaloids of pyrrolizidine already described in this species by Faqueti et al (2017). [5] Many signs of alpha-oxygenated aromatic hydrogens have also been observed, which may be derived from compounds such as phenolic acids, coumarin, benzopyrones, chlorogenic acid, coumaric acid, tannins and other phenols already reported in this species, as well as several aromatic methoxy hydrogens compounds such as polymethoxyflavones.…”

Section: Discussionmentioning

confidence: 57%

“…"Erva de São João" (Ageratum conyzoides L.) is a plant largely used in traditional medicine of tropical and subtropical regions for its anti-inflammatory and antinociceptive properties. [5] "Bardana" (Arctium lappa Linné), a vegetable that has long been used as a diuretic, hypertension, gout, arteriosclerosis, hepatites and others inflammatory disorders. [6] A number of Plantago spp.…”

Section: Introductionmentioning

confidence: 99%

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Bioactivity and Dereplication of Phenolic Compounds in Medicinal Plants

Lima

Silveira

Ramos

et al. 2019

Int. J. Pharm. Sci. Drug Res.

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Three medicinal plants with recognized anti-inflammatory potential, identified as “erva de São João“ (Ageratum conyzoides), “Tanchagem“ (Plantago major) and “ Bardana“ (Arctium lappa L.) were obtained from a medicinal herbs company located in Teófilo Otoni city (Minas Gerais State, Brazil). The dry plant material obtained in packages was submitted to the chemical procedures to prepare the crude extracts by maceration according to the Brazilian pharmacopoeia legislation. After extraction, the samples were subjected to 1H NMR, TLC and Capillary Electrophoresis analysis by co-injection of authentic patterns of phenolic acids and flavonoids to identify the major compounds and classes of secondary metabolites present in each material and then their chemical and biological potential was assessed by DPPH free radical inhibition assay and antimicrobial against E. coli. The results obtained allow us to conclude that the phytopreparation was effective in the extraction of compounds with antioxidant potential and the three species presented a high concentration of flavonoids and other phenolics that is compatible with the chemosystematic data. The screening obtained by 1H NMR spectroscopy, TLC and Capillary Electrophoresis with ultraviolet detection analysis provided us a qualitative profile of the phytochemicals present in each material. None of the extracts were active against Escherichia coli by antibacterial disk diffusion assay at concentration of 1 mg/ml.

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Section: Discussionmentioning

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Bioactivity and Dereplication of Phenolic Compounds in Medicinal Plants

Lima

Silveira

Ramos

et al. 2019

Int. J. Pharm. Sci. Drug Res.

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“…Several signals were detected from the alphaoxygenated aromatic hydrogens, which were present in compounds such as phenolic acids, coumarins, benzopyrones, chlorogenic acids, coumaric acids, tannins, and other phenols previously reported in this species, and also in several aromatic methoxy hydrogen compounds such as polymethoxyflavones (Sultana et al, 2012;Faqueti et al, 2017). Therefore, the presence of carbinolic, ester, and aliphatic hydrogens observed in the spectra of these samples, suggested the presence of compounds such as sesquiterpene lactones, previously described in this species (Chagas-Paula et al, 2015).…”

Section: Comparison Of Effectiveness Of the Chemical Marker Extraction Methods Of The Medicinal Herbs By 1 H Nmr And Rp-hplc-pdamentioning

confidence: 59%

“…Additionally, the presence of aromatic hydrogens was detected at around 8.0 ppm, possibly arising from the peri-carbonylic hydrogens. Singlets were observed at around 9.0 ppm, possibly due to the presence of nitrogen in the aromatic alkaloids, such as the toxic pyrrolizidine alkaloids previously described in this species by Faqueti et al (2017). The chemical composition of AC has not yet been accurately.…”

Section: Comparison Of Effectiveness Of the Chemical Marker Extraction Methods Of The Medicinal Herbs By 1 H Nmr And Rp-hplc-pdamentioning

confidence: 88%

“…Regarding the chemodiversity of the species evaluated, e.g., the AC particularly present pyrrolizidine alkaloids (Faqueti et al, 2017), phenolic acids, coumarins, benzopyrones, chlorogenic acid, coumaric acid, tannins, polymethoxyflavones (Sultana et al, 2012;Faqueti et al, 2017;), sesquiterpene lactones (Chagas-Paula et al, 2015), glycosylated flavonoids (Munikishore et al, 2013), terpenoids, chromenes, and also phytol (Vera, 1993). In the case of AL species are described flavonoids (Ionescu et al, 2014), phenylpropanoids (Gao et al, 2013), and phenolic acids derived from caffeic acid such as dicaffeoylquinic acid (Carlotto et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Effectiveness of different methods for the extraction of principle actives and phytochemicals content in medicinal herbals

Lima¹,

Falcoski²,

Silveira³

et al. 2021

BLACPMA

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In this present study, we investigated the influence of various extraction methods including maceration, sonication, infusion, decoction, and microwave extraction, on the chemical and biological potential of phytochemicals extracted from three medicinal plants (Ageratum conyzoides, Plantago major and Arctium lappa L). The results were subsequently analyzed by variance analysis. Our results suggested that sonication is the most effective extraction method among the five methods tested herein, for the extraction of phytochemicals that have a high antioxidant potential and high phenolic content. The three plants employed for this study had a high concentration of flavonoids and phenolics which was compatible with the chemosystematics of the species. All the samples possessed a Sun Protection Factor (SPF) of less than 6. Interestingly, a maximum reaction time of approximately 20 min was noted for the complexation of AlCl3with the flavonoids present in the phytochemical extract during analyses of the kinetic parameters. We finally identified that the Ageratum conyzoides extract, prepared by sonication, possessed a significant pharmacological potential against hepatocarcinoma tumour cells, whose result can guide further studies for its therapeutic efficacy.

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Authenticity study of commercial samples of St. John's wort by paper spray ionization mass spectrometry and chemometric tools

et al. 2023

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Hypericum perforatum L. (St. John's wort) is one of the world's most consumed medicinal plants for treating depression and psychiatric disorders. Counterfeiting can occur in the medicinal plant trade, either due to the lack of active ingredients or the addition of substances not mentioned on the labels, often without therapeutic value or even harmful to health. Hence, 43 samples of St. John's wort commercially acquired in different Brazilian regions and other countries were analyzed by paper spray ionization mass spectrometry (PS-MS) and modeled by principal component analysis. Hence, samples (plants, capsules, and tablets) were extracted with ethanol in a solidliquid extraction. For the first time, PS-MS analysis allowed the detection of counterfeit H. perforatum samples containing active principles typical of other plants, such asAgeratum conyzoides and Senna spectabilis. About 52.3% of the samples were considered adulterated for having at least one of these two species in their composition.Furthermore, out of 35 samples produced in Brazil, only 13 were deemed authentic, having only H. perforatum. Therefore, there is a clear need to improve these drugs' quality control in Brazil.

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Simultaneous identification and quantification of polymethoxyflavones, coumarin and phenolic acids in Ageratum conyzoides by UPLC-ESI-QToF-MS and UPLC-PDA

Cited by 24 publications

References 29 publications

Bioactivity and Dereplication of Phenolic Compounds in Medicinal Plants

Bioactivity and Dereplication of Phenolic Compounds in Medicinal Plants

Effectiveness of different methods for the extraction of principle actives and phytochemicals content in medicinal herbals

Authenticity study of commercial samples of St. John's wort by paper spray ionization mass spectrometry and chemometric tools

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