Simultaneous HPLC-DAD determination of seven intense sweeteners in foodstuffs and pharmaceuticals using a core-shell particle column

Sezgin, Burcu; Arlı, Göksel; Can, Nafiz Öncü

doi:10.1016/j.jfca.2020.103768

Cited by 10 publications

(2 citation statements)

References 19 publications

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“…Other methods of analysis of E 959 in food described in the scientific literature are LC‐based methods using evaporative light‐scattering detection (ELSD) (Wasik et al., 2007; Buchgraber and Wasik, 2009) and (photo)‐diode array and charged aerosol detection systems (Ma et al., 2020; Sezgin et al., 2021). Also, the use of supercritical fluid chromatography coupled with ELSD was investigated for the determination of the sweetener in soft drinks (Lefler and Chen, 2008).…”

Section: Assessmentmentioning

confidence: 99%

Re‐evaluation of neohesperidine dihydrochalcone (E 959) as a food additive

Younes¹,

Aquilina²,

Castle³

et al. 2022

EFS2

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The present opinion deals with the re‐evaluation of neohesperidine dihydrochalcone (E 959) when used as a food additive. It is obtained by catalytic hydrogenation of a flavanone – neohesperidine – which is naturally occurring and thus isolated by alcohol extraction in bitter oranges (Citrus aurantium). Based on in vivo data in rat, neohesperidine dihydrochalcone is likely to be absorbed, also in humans, and to become systemically available. It does not raise a concern regarding genotoxicity. The toxicity data set consisted of studies on subchronic and prenatal developmental toxicity. No human studies were available. The data set was considered sufficient to derive a new acceptable daily intake (ADI). Based on the weight of evidence (WoE) analysis, the Panel considered unlikely that neohesperidine dihydrochalcone would lead to adverse effects on health in animals in the dose ranges tested. The Panel also considered that a carcinogenicity study was not warranted and that the lack of human data did not affect the overall confidence in the body of evidence. The Panel derived an ADI of 20 mg/kg bodyweight (bw) per day based on a no observed adverse effect level (NOAEL) of 4,000 mg/kg bw per day from a 13‐week study in rat, applying the standard default factors of 100 for inter‐ and intraspecies differences and of 2 for extrapolation from subchronic to chronic exposure. For the refined brand‐loyal exposure assessment scenario, considered to be the most appropriate for the risk assessment, the exposure estimates at the mean ranged from < 0.01 to 0.09 mg/kg bw per day and at the 95th percentile (P95) from 0.01 to 0.24 mg/kg bw per day. Considering the derived ADI of 20 mg/kg bw per day, the exposure estimates were below the reference value in all age groups. Therefore, the Panel concluded that dietary exposure to the food additive neohesperidine dihydrochalcone (E 959) at the reported uses and use levels would not raise a safety concern.

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Section: Assessmentmentioning

confidence: 99%

Re‐evaluation of neohesperidine dihydrochalcone (E 959) as a food additive

Younes¹,

Aquilina²,

Castle³

et al. 2022

EFS2

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“…Bir sıvıda çözünebilen bileşenlerin (ilaç etken maddeleri, aminoasitler, şekerler, vitaminler, antibiyotikler, pestisitler, gıda katkı maddeleri, gıda toksinleri vb.) bu teknik ile nitel ve nicel olarak hassas, hızlı ve güvenilir şekilde tespit edilmesi mümkündür (Sezgin, Arli, & Can, 2021;Soyseven, Kaynak, Çelebier, Aboul-Enein, & Arli, 2020, Moldoveanu & David, 2002Moldoveanu & David, 2013a;Snyder, Kirkland, & Dolan, 2011).…”

Section: Introductionunclassified

Piroksikamın pKa Değerinin Yüksek Performanslı Sıvı Kromatografisi ile Belirlenmesi

Soyseven¹,

Sezgin²,

Arlı³

2022

European Journal of Science and Technology

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ÖzBu çalışmada bir nonsteroid anti-inflamatuvar ilaç olan piroksikamın ters faz sıvı kromatografi yöntemiyle farklı asetonitril-su ikili karışımlarındaki ve sudaki asidik iyonlaşma sabitleri (pKa) belirlenmiştir. Piroksikamın kromatografik davranışına hareketli fazın pH'ı ve çözücü yüzdesinin birleştirilmiş etkisini incelemek amacıyla SVEA C18 (5 μm, 150 mm x 4.6 mm) YPSK analiz kolonu ve farklı oranda asetonitril-su (%30, %35 ve %40 (h/h)) ikili karışımlarından oluşan, farklı pH değerlerine (3,13-6,18) sahip hareketli fazlar kullanılmıştır. Asidik iyonlaşma sabitleri doğrusal olmayan regresyon modeli kullanılarak tahmin edilmiştir. Piroksikamın sudaki pKa değeri mol kesri-pKa yöntemiyle hesaplanmıştır. Veriler literatürde farklı tekniklerle elde edilmiş olanlarla kıyaslanmış ve uyumlu olduğu görülmüştür.

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Optimization and validation of HPLC–DAD method for simultaneous analysis of sweeteners, preservatives, and caffeine in sugar-free beverages

Székelyhidi,

Ajtony,

Lakatos

et al. 2023

Eur Food Res Technol

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In this study, the optimization and performance evaluation of a high-performance liquid chromatography with diode array detection (HPLC–DAD) method for the simultaneous determination of four sweeteners (acesulfame-potassium, saccharin, aspartame, and rebaudioside A), two preservatives (sodium benzoate and potassium sorbate), and caffeine in sugar-free drinks are presented. The separation was carried out using a gradient elution of acetonitrile and phosphate buffer (12.5 mM, pH = 3.3) on a reversed-phase column. Under the optimized conditions, the method allowed for the specific and selective simultaneous separation of all target analytes in less than 9 min. The developed HPLC–DAD method was validated and demonstrated excellent linearity (all analytical curves showed R2 ≥ 0.9995), satisfactory accuracy (recovery values ranging between 94.1 and 99.2% in real samples), and repeatability (intra- and inter-day relative standard deviations were ≤ 2.49%). Overall, 69 products available in the Hungarian market were successfully tested with the applied method.

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Simultaneous HPLC-DAD determination of seven intense sweeteners in foodstuffs and pharmaceuticals using a core-shell particle column

Cited by 10 publications

References 19 publications

Re‐evaluation of neohesperidine dihydrochalcone (E 959) as a food additive

Re‐evaluation of neohesperidine dihydrochalcone (E 959) as a food additive

Piroksikamın pKa Değerinin Yüksek Performanslı Sıvı Kromatografisi ile Belirlenmesi

Optimization and validation of HPLC–DAD method for simultaneous analysis of sweeteners, preservatives, and caffeine in sugar-free beverages

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