Simultaneous HPLC Analysis of Betamethasone and Clotrimazole in Cream Formulations

Manassra, Adnan; Khamis, Mustafa; el-Dakiky, Magdy; Abdel-Qader, Zuhair; Al‐Rimawi, Fuad

doi:10.4172/2153-2435.1000113

Cited by 17 publications

(14 citation statements)

References 11 publications

(18 reference statements)

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“…developed a simultaneous HPLC analysis of betamethasone and clotrimazole to quantify drug content in cream formulations. The linearity range of the developed method was 25–250 µg/mL . Lotfy et al.…”

Section: Introductionmentioning

confidence: 95%

Stability indicating liquid chromatographic method for simultaneous quantification of betamethasone valerate and tazarotene in in vitro and ex vivo studies of complex nanoformulation

Rapalli

Singhvi

Gorantla

et al. 2019

J of Separation Science

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Low‐potency corticosteroid betamethasone valerate and vitamin‐A tazarotene are used in combination for effective treatment of psoriasis. There is no robust high‐performance liquid chromatography analytical technique available for simultaneous estimation of betamethasone valerate and tazarotene in conventional and nanocarriers based formulations. A simple, accurate, robust isocratic high‐performance liquid chromatography method was developed for simultaneous estimation of betamethasone valerate and tazarotene in topical pharmaceutical formulations. The developed method was validated as per the regulatory guidelines. The validated method was linear over the concentration range of 150–6000 ng/mL (r2 > 0.999) at 239 nm wavelength. Limits of detection and quantification of two analytes were 50 and 150 ng/mL, respectively. The %relative standard deviation for intraday and interday precision was less than 2%. The method was also evaluated in the presence of forced degradation conditions. The developed method was successfully applied for in vitro and ex vivo drug release studies of in‐house designed nanoformulations.

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Section: Introductionmentioning

confidence: 95%

Stability indicating liquid chromatographic method for simultaneous quantification of betamethasone valerate and tazarotene in in vitro and ex vivo studies of complex nanoformulation

Rapalli

Singhvi

Gorantla

et al. 2019

J of Separation Science

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“…Whereas different HPLC analytical method for estimation of clotrimazole from cream are available in literature but they have complicated method of sample and standard preparation [11], estimation is done for lozenges dosage form by Gracemart C18 column [12], separation is done by using Kromasil C18 with maintaining 40°C temperature [13]. …”

Section: Methods Optimization and Validationmentioning

confidence: 99%

Modification and Validation of HPLC Analytical Method for Estimation of Clotrimazole in Health Care Products

Usman¹,

Zulfiqar²,

Shah³

et al. 2017

Nat Prod Chem Res

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Objective: The aim of the study was to modify the USP analytical method and validate it for the estimation of clotrimazole in health care cream by using reverse phase HPLC.Methods: LC-20AD liquid chromatography equipped with SPD-20A UV-VIS detector was used for analysis. The separation was done on C18 column which was attached with loop 20 µL. Mobile phase was consisting of methanol: K 2 HPO 4 (85:15) and flow rate was 1.5 mL/min. The method was validated as per the ICH guidelines. Results:The method is selective and reproducible, capable to separate all possible excipients and help to quantify accurately the amount of clotrimazole in health care product. The percentage coefficient variation (% CV) of the retention times and the peak areas were 0.504% and 1.453%, respectively. The linearity was evaluated by least square method i.e., r 2 =0.9996. The limits of detection (LOD) and limits of quantitation (LOQ) for clotrimazole were 0.25 µg/mL and 0.5 µg/mL respectively. The results of Instrumental precision were 103.5% with %CV of 0.612% and the intermediate precision was 0.35 to 1.43%. The recoveries at three concentrations levels over a range of 80 -120% were found within the range of 101.2 to 103.8% as per ICH guidelines. The results of robustness show no major changes occurring over changes in flow rate from 1.4 mL/min. to 1.6 mL/min. The method was also applied for the analysis of clotrimazole in formulated health care cream and marketed pharmaceutical creams that contain clotrimazole. Conclusion:It was concluded that the method is so simple, easy and applicable. It could be easily used for the analysis of compound in its dosage form. The robustness and ruggedness of the method are significantly broader, reproducible and could be adjusted according the needs.

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“…They include UV spectrophotometric methods [1] , HPLC. [2][3][4][5][6][7] Fusidic acid is designated chemically ent-16α-acetoxy-3β,11β-dihydroxy-4β,8β,14β-trimethyl-18-nor-5β,10α-cholesta(17Z) formulation, as well as in combination with other drugs. They include simple spectrophotometric methods [8] , HPLC methods.…”

Section: Devanshi Et Al World Journal Of Pharmacy and Pharmaceuticalmentioning

confidence: 99%

Method Development and Validation for Estimation of Clotrimazole, Fusidic Acid and Mometasone Furoate in Cream by Rp-Hplc.

Trivedi¹

2017

WJPPS

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Simultaneous HPLC Analysis of Betamethasone and Clotrimazole in Cream Formulations

Cited by 17 publications

References 11 publications

Stability indicating liquid chromatographic method for simultaneous quantification of betamethasone valerate and tazarotene in in vitro and ex vivo studies of complex nanoformulation

Stability indicating liquid chromatographic method for simultaneous quantification of betamethasone valerate and tazarotene in in vitro and ex vivo studies of complex nanoformulation

Modification and Validation of HPLC Analytical Method for Estimation of Clotrimazole in Health Care Products

Method Development and Validation for Estimation of Clotrimazole, Fusidic Acid and Mometasone Furoate in Cream by Rp-Hplc.

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