Simultaneous high-performance liquid chromatographic determination of salicylates in whole blood, plasma and isolated erythrocytes

Sensitive determination of aspirin and its metabolites in plasma by LC–UV using on-line solid-phase extraction with methylcellulose-immobilized anion-exchange restricted access media

“…Most of these methods utilized an off-line sample procedure involving solvent extraction and therefore required evaporation and reconstitution steps. Since evaporation leads to the loss of ASA and/or SA because of sublimation [24,32,35,[44][45][46][47], the use of such procedures generally results in reduced recovery of the compounds being analyzed.…”

“…SA is further metabolized to gentisic acid (GA), salicyluric acid (SUA), and other conjugates [22]. A number of methods have been reported for the analysis of ASA and its metabolites in plasma samples, including LC [23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38], GC [39][40][41][42], and capillary electrophoresis [43]. Most of these methods utilized an off-line sample procedure involving solvent extraction and therefore required evaporation and reconstitution steps.…”

Determination of Triflusal in Human Plasma by High Performance Liquid Chromatography With Automated Column Switching System

“…Figure 5 shows a mean plasma concentration versus time plot of triflusal following an oral administration (300 mg) to human subjects. 12 The present method offers practical advantages over the liquid-liquid 13,14 or solid-phase extraction 7 in the viewpoint of the speed, sensitivity, sample throughput, and sample volume. In general, triflusal in plasma TRIFLUSAL IN HUMAN PLASMA 2519 samples could be readily analyzed between 10 min (the first sampling time, 0.16 ± 0.07 µg/mL) to 240 min (the last sampling time, 0.12 ± 0.08 µg/mL) after the oral administration.…”

Combined liquid and solid-surface room temperature fluorimetric determination of naproxen and salicylate in serum

“…If this statement is taken into account, it can be claimed that the proposed method satisfactorily compares with the sensitivity of other described techniques. Limits of detection ranging between 0.01-0.1 g ml −1 [4,7,8], and quantitation of 0.1-4.3 g ml −1 [6,9,10] have been reported for the studied compounds using HPLC. On the other hand, limits of detection of 0.03 and 0.05 g ml −1 for NX and SA, respectively, have been described using techniques involving multivariate calibration [21].…”

“…Several high-performance liquid chromatographic (HPLC) methods have been proposed for the evaluation of NX [4][5][6], SA [7][8][9][10] or both [11,12] in serum or plasma samples. Because of the luminescence properties of both active principles, various analytical methodologies using this type of signals have also been reported [13][14][15][16][17][18][19].…”