Simultaneous FTIR and Raman Spectroscopy in Endometrial Atypical Hyperplasia and Cancer

Barnaś, Edyta; Skręt-Magierło, Joanna; Kaznowska, Ewa; Depciuch, Joanna; Szmuc, K.; Łach, Kornelia; Krawczyk-Marć, Izabela; Cebulski, J.

doi:10.3390/ijms21144828

Cited by 21 publications

(18 citation statements)

References 43 publications

(48 reference statements)

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“…The difficulties of differential diagnosis of prostate cancer and precancerous processes contribute to the development and introduction into clinical practice of the latest diagnostic methods based on the use of high-tech and sensitive equipment. At present, reports on the use of laser optical spectroscopy in the diagnosis of a wide number of diseases are increasingly appearing in the world literature [ 5 , 38 , 39 , 40 , 41 , 42 , 43 ]. Compared to traditional imaging methods (Magnetic Resonance Imaging and Transrectal Ultrasound Diagnostics), optical methods have a number of unique advantages.…”

Section: Discussionmentioning

confidence: 99%

Using the Method of “Optical Biopsy” of Prostatic Tissue to Diagnose Prostate Cancer

et al. 2021

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The possibilities of using optical spectroscopy methods in the differential diagnosis of prostate cancer were investigated. Analytical discrimination models of Raman spectra of prostate tissue were constructed by using the projections onto latent structures data analysis(PLS-DA) method for different wavelengths of exciting radiation—532 and 785 nm. These models allowed us to divide the Raman spectra of prostate cancer and the spectra of hyperplasia sites for validation datasets with the accuracy of 70–80%, depending on the specificity value. Meanwhile, for the calibration datasets, the accuracy values reached 100% for the excitation of a laser with a wavelength of 785 nm. Due to the registration of Raman “fingerprints”, the main features of cellular metabolism occurring in the tissue of a malignant prostate tumor were confirmed, namely the absence of aerobic glycolysis, over-expression of markers (FASN, SREBP1, stearoyl-CoA desaturase, etc.), and a strong increase in the concentration of cholesterol and its esters, as well as fatty acids and glutamic acid. The presence of an ensemble of Raman peaks with increased intensity, inherent in fatty acid, beta-glucose, glutamic acid, and cholesterol, is a fundamental factor for the identification of prostate cancer.

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Section: Discussionmentioning

confidence: 99%

Using the Method of “Optical Biopsy” of Prostatic Tissue to Diagnose Prostate Cancer

et al. 2021

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“…For this purpose, Raman and FTIR spectroscopy can be used to obtain information about chemical changes. Importantly, our previous study demonstrated the potential of FT-Raman and FTIR spectroscopy for the identification of chemical changes in atypical complex hyperplasia and endometrioid adenocarcinoma cancer 4 . To the best of our knowledge, this is the first study applying physical methods such as FT-Raman and FTIR spectroscopy with multidimensional analysis and correlation test to evaluate carcinogenesis in endometrial cancer.…”

Section: Introductionmentioning

confidence: 97%

“…Spectroscopy candidates for this role. Specimens for both pathological spectroscopic analysis of stages of endometrial cancer carcinogenesis and tissues adjacent cancer are easily available in curettage 4 . Another aim of spectroscopic analysis of stages preceding endometrial cancer may be to determine a new mechanism operating behind the initiation of carcinogenesis.…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, it is of prime importance to conduct interdisciplinary studies e.g. in physics to develop methods which can be used in diagnostic of cancer diseases 4 , 12 – 14 . Some of these methods are Raman and FTIR spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

“…Spectroscopy could be used as a comparative for gold standard methods facilitating clinical decision-making and patient outcomes by detecting biochemical changes in tissues before the changes can be observed under microscope. Nowadays, Raman and FTIR spectroscopy methods are used to detect chemical changes caused by lung 12 , 15 , bones 16 , 17 , thyroid 13 , 18 , breast 19 , prostate 20 , endometrial cancers 4 . However, recognizing changes visible during neoplastic process is important in the cancer diagnostics.…”

Section: Introductionmentioning

confidence: 99%

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Spectroscopic evaluation of carcinogenesis in endometrial cancer

Depciuch

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Skręt-Magierło

et al. 2021

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Carcinogenesis is a multifaceted process of cancer formation. The transformation of normal cells into cancerous ones may be difficult to determine at a very early stage. Therefore, methods enabling identification of initial changes caused by cancer require novel approaches. Although physical spectroscopic methods such as FT-Raman and Fourier Transform InfraRed (FTIR) are used to detect chemical changes in cancer tissues, their potential has not been investigated with respect to carcinogenesis. The study aimed to evaluate the usefulness of FT-Raman and FTIR spectroscopy as diagnostic methods of endometrial cancer carcinogenesis. The results indicated development of endometrial cancer was accompanied with chemical changes in nucleic acid, amide I and lipids in Raman spectra. FTIR spectra showed that tissues with development of carcinogenesis were characterized by changes in carbohydrates and amides vibrations. Principal component analysis and hierarchical cluster analysis of Raman spectra demonstrated similarity of tissues with cancer cells and lesions considered precursor of cancer (complex atypical hyperplasia), however they differed from the control samples. Pearson correlation test showed correlation between cancer and complex atypical hyperplasia tissues and between non-cancerous tissue samples. The results of the study indicate that Raman spectroscopy is more effective in assessing the development of carcinogenesis in endometrial cancer than FTIR.

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Altered type I collagen networking in osteoporotic human femoral head revealed by histomorphometric and Fourier transform infrared imaging correlated analyses

et al. 2022

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Bone homeostasis is the equilibrium between organic and inorganic components of the extracellular matrix (ECM) and cells. Alteration of this balance has consequences on bone mass and architecture, resulting in conditions such as osteoporosis (OP). Given ECM protein mutual regulation and their effects on bone structure and mineralization, further insight into their expression is crucial to understanding bone biology under normal and pathological conditions. This study focused on Type I Collagen, which is mainly responsible for structural properties and mineralization of bone, and selected proteins implicated in matrix composition, mineral deposition, and cell‐matrix interaction such as Decorin, Osteocalcin, Osteopontin, Bone Sialoprotein 2, Osteonectin and Transforming Growth Factor beta. We developed a novel multidisciplinary approach in order to assess bone matrix in healthy and OP conditions more comprehensively by exploiting the Fourier Transform Infrared Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting. This innovatory procedure allowed for the analysis of superimposed tissue sections and revealed that the alterations in OP bone tissue architecture were associated with warped Type I Collagen structure and deposition but not with changes in the total protein amount. The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein‐2 indicate the deep impact of these NCPs on collagen features of OP bone. Overall, our strategy may represent a starting point for designing targeted clinical strategies aimed at bone mass preservation and sustain the FTIRI translational capability as upcoming support for traditional diagnostic methods.

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Simultaneous FTIR and Raman Spectroscopy in Endometrial Atypical Hyperplasia and Cancer

Cited by 21 publications

References 43 publications

Using the Method of “Optical Biopsy” of Prostatic Tissue to Diagnose Prostate Cancer

Using the Method of “Optical Biopsy” of Prostatic Tissue to Diagnose Prostate Cancer

Spectroscopic evaluation of carcinogenesis in endometrial cancer

Altered type I collagen networking in osteoporotic human femoral head revealed by histomorphometric and Fourier transform infrared imaging correlated analyses

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