Simultaneous fluoroimmunoassay of two tumor markers based on CdTe quantum dots and gold nanocluster coated-silica nanospheres as labels

Li, Lihua; Feng, Dexiang; Zhao, Junqing; Guo, Zilin; Zhang, Yuzhong

doi:10.1039/c5ra19262e

Cited by 14 publications

(8 citation statements)

References 36 publications

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“…Apart from screening techniques, breast biopsies are generally performed to distinguish between cancerous and benign tissues [ 22 ], but this is an expensive method that requires trained people [ 23 ]. Biomarker-based methods such as radioimmunoassay, immunohistochemistry, enzyme-linked immunosorbent assay (ELISA) and fluoroimmunoassay also cater to the diagnostic requirements for breast cancer [ 24 , 25 ]. Biomarker-based techniques are sensitive and selective, however, they have some limitations such as being expensive, time consuming, needing trained people and complex labeling process are also required [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Early Diagnosis of Breast Cancer

Wang

2017

Sensors

362

220

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Early-stage cancer detection could reduce breast cancer death rates significantly in the long-term. The most critical point for best prognosis is to identify early-stage cancer cells. Investigators have studied many breast diagnostic approaches, including mammography, magnetic resonance imaging, ultrasound, computerized tomography, positron emission tomography and biopsy. However, these techniques have some limitations such as being expensive, time consuming and not suitable for young women. Developing a high-sensitive and rapid early-stage breast cancer diagnostic method is urgent. In recent years, investigators have paid their attention in the development of biosensors to detect breast cancer using different biomarkers. Apart from biosensors and biomarkers, microwave imaging techniques have also been intensely studied as a promising diagnostic tool for rapid and cost-effective early-stage breast cancer detection. This paper aims to provide an overview on recent important achievements in breast screening methods (particularly on microwave imaging) and breast biomarkers along with biosensors for rapidly diagnosing breast cancer.

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Section: Introductionmentioning

confidence: 99%

Early Diagnosis of Breast Cancer

Wang

2017

Sensors

362

220

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“…Although quantum dots have high fluorescence efficiency and photostability, they are easy to agglomerate as metal nanoclusters, which leads to the decrease of fluorescence intensity. To solve this problem, Li et al , 89 designed CdTe quantum dots (CdTe QDs) and gold nanoclusters (Au NCs) coated-silica nanospheres as labels. After sandwich immune reaction with immunomagnetic beads, carcinoembryonic antigen (CEA) was detected by fluorescence method.…”

Section: Fluorescence Immunosensor Based On Functional Nanomaterials ...mentioning

confidence: 99%

Fluorescence immunosensor based on functional nanomaterials and its application in tumor biomarker detection

et al. 2022

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“…Because of their small size, large specific surface area, and good stability at high temperatures, nanoparticles have been widely used for signal amplifications including colloidal gold nanoparticles, magnetic nanoparticles, carbon nanospheres, quantum dots, silicon dioxide nanospheres (SiO 2 NSs), etc. [21][22][23][24][25]. More importantly, the surfaces of these nanoparticles can be modified with oligonucleotides, enzymes, or antibodies to generate biological conjugates that serve as analysis cores and enhance signal generation by producing a synergistic effect to achieve higher sensitivity and lower detection limits [26,27].…”

Section: Introductionmentioning

confidence: 99%

A Sensitive Fluorescent Immunoassay for Prostate Specific Antigen Detection Based on Signal Amplify Strategy of Horseradish Peroxidase and Silicon Dioxide Nanospheres

Zhang

et al. 2022

Journal of Analytical Methods in Chemistry

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A simple, sensitive, and fluorescent immunoassay for the detection of prostate-specific antigen (PSA) based on horseradish peroxidase and silicon dioxide nanospheres as a signal amplification strategy has been described. In the design, the primary antibody (Ab1) of PSA was first immobilized on the 96-well plates via physical adsorption between polystyrene and hydrophobic groups of the antibody molecule. The silicon dioxide nanospheres (SiO2 NSs), with large surface area and good biocompatibility, were loaded with horseradish peroxidase (HRP) and horseradish peroxidase-labeled secondary antibodies (HRP-Ab2) as signal amplification systems. In the presence of PSA, a sandwich-type immunocomplex composed of Ab1-Ag-HRP-Ab2 had been formed. Fluorescence technology was employed to obtain the response signal of the immunoassay in the L-tyrosine and H2O2 systems. Experiment results showed that a strong and stable fluorescent signal at 416 nm (excitation wavelength: 325 nm) was observed, and the changes in fluorescent intensity were related to the levels of PSA. Under the optimal conditions, the relative fluorescence intensity was linear with the logarithm of PSA concentration from 0.03 to 100 ng·mL−1, with a detection limit of 0.01 ng·mL−1 (at a signal/noise ratio of 3). In contrast to other fluorescent immunoassays, the sandwich-type immunoreaction based on the high binding ELISA plates has the advantages of being simple, stable, and easy to operate, high selectivity, small sample quantity, etc., which can be widely used in the selective detection of a variety of targets, from DNA to proteins and small molecules. Such fluorescent immunoassays should be feasible for the fields of molecular diagnosis and other life science fields in the future.

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Simultaneous fluoroimmunoassay of two tumor markers based on CdTe quantum dots and gold nanocluster coated-silica nanospheres as labels

Abstract: Fluoroimmunoassay for simultaneous detection of CEA and AFP.

Cited by 14 publications

References 36 publications

Early Diagnosis of Breast Cancer

Early Diagnosis of Breast Cancer

Fluorescence immunosensor based on functional nanomaterials and its application in tumor biomarker detection

A Sensitive Fluorescent Immunoassay for Prostate Specific Antigen Detection Based on Signal Amplify Strategy of Horseradish Peroxidase and Silicon Dioxide Nanospheres

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