Simultaneous extraction of oil and protein from perilla seed by three-phase partitioning and their application in serum

Pintathong, Punyawatt; Chaiwut, Phanuphong; Thitipramote, Natthawut; Thitilertdecha, Nont; Nantitanont, Witayapan; Sangthong, Sarita; Tiensri, Ninnapat

doi:10.14416/j.appsci.2018.02.s09

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…Additionally, perilla is not only used as a traditional medicine and functional food throughout Asian countries [3], but also as a fundamental cosmetic ingredient [4,5]. Perilla also gained market importance Cosmetics 2020, 7, 69 2 of 10 in cosmetics according to its biological activities; different parts of perilla have been differently processed in skin aging creams, serums, gels, and dermatological medicinal preparations [3,6]. The leaves and seeds contain high nutritional value.…”

Section: Introductionmentioning

confidence: 99%

Collagenase and Melanogenesis Inhibitory Effects of Perilla Frutescens Pomace Extract and Its Efficacy in Topical Cosmetic Formulations

et al. 2020

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In previous studies, Perilla frutescens pomace was shown to contain bioactive phenolic compounds and good anti-oxidative activity. However, reports about collagenase activity and melanogenesis inhibitory effects of P. frutescens pomace are limited. This study aimed to evaluate the bioactivity of P. frutescens pomace extract and incorporate the extract into a cosmetic formulation for evaluating its effects on collagenase and melanogenesis inhibition on human skin. The P. frutescens seeds after an oil pressing process were extracted with ethanol (70% v/v) in order to examine the remaining phytochemical compounds, the bioactivity in pomace perilla, and its efficacy as a skincare product. In this study, total phenolic and total flavonoid contents of P. frutescens seed extract (PFSE) were determined using spectrophotometry. The free radical scavenging activity was determined with 2, 2′-diphenyl-1-picrylhydrazyl (DPPH) radicals and 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) ABTS radicals. Additionally, the effects on collagenase activity, melanin content, and alpha-Melanocyte stimulating hormone (α-MSH) on the viability of cultured B16F10 melanoma cells were investigated. Skin irritation and efficacy of PFSE cream for skin elasticity and skin color were also clinically evaluated. The total phenolic content with gallic acid equivalents (GAE) value and total flavonoids content with catechin equivalents (CE) value were, respectively, 92.79 ± 1.19 and 56.02 ± 2.83 mg/g. Furthermore, PFSE significantly inhibited the collagenase activity (p < 0.001) at the concentration of 400 µg/mL (82 ± 3.23%). These results clearly demonstrated the anti-melanogenic effects on B16F10 cells without causing any cytotoxicity or death. Although there was a slight improvement in skin elasticity in the 4th week compared to the previous week, the 4th week melanin content of the skin significantly decreased from the beginning (p < 0.05) without any irritations. In conclusion, PFSE could be cosmetically considered as a key ingredient that effectively lessens the effects of skin aging and skin hyperpigmentation disorders.

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Section: Introductionmentioning

confidence: 99%

Collagenase and Melanogenesis Inhibitory Effects of Perilla Frutescens Pomace Extract and Its Efficacy in Topical Cosmetic Formulations

et al. 2020

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Green Synthesis Optimization of Glucose Palm Oleate and Its Potential Use as Natural Surfactant in Cosmetic Emulsion

et al. 2022

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This study aimed to optimize the green synthesis of glucose palm oleate catalyzed by Carica papaya Lipase (CPL) through transesterification in a solvent-free system. Palm olein was used as a fatty acid donor for transesterification reactant and was also employed as a reaction medium. Reaction optimization was performed by using response surface methodology (RSM). Seventeen synthesis conditions were generated by a Box–Behnken design and the products were further determined by ultra-performance liquid chromatography (UPLC). Fatty acid compositions of palm olein identified by gas chromatography-mass spectrometry (GC-MS) found that oleic acid (51.77 ± 0.67%) and palmitic acid (37.22 ± 0.48%) were major components. The synthesis variable factors of 50 °C, 45 h reaction time, and 1400 U of CPL were predicted by the RSM to be optimum conditions and thus provided the highest glucose palm oleate of 0.3542 mmol/g. Conjugation between palm olein fatty acids and glucose via transesterification resulted in glucose palm oleate being obviously verified by UPLC, Fourier-transform infrared spectroscopy (FTIR), and thin-layer chromatography (TLC) analyses. The synthesized sugar fatty acid ester revealed an HLB value of 6.20 represented by the lowest % creaming index (%CI) of 35.40 ± 3.21%. It also exhibited a critical micelle concentration (CMC) of 3.16 × 10−5 M. This study is the first report to reveal the transesterification of glucose and palm olein catalyzed by CPL in a system without using any solvent. Glucose palm oleate has been shown to be derived from an environmentally friendly synthesis process and would be promising as a potential alternative natural surfactant for cosmetic application.

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Simultaneous Extraction of Oil and Protein from Silkworm (Bombyx mori L.) Pupae (Lueng Parroj var.) and Their In Vitro Skin Moisturization

Susirirut,

Thitipramote,

Chaiwut

2023

Molecules

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Oil and protein from silkworm (Bombyx mori var. Leung Pairoj) pupae, by-product from sericulture, were extracted and evaluated for their potential uses as skin biomoisturizer. The silkworm pupae (SWP) oil and protein were simultaneously extracted by using three-phase partitioning (TPP) method and determined for their physicochemical properties including fatty acid and amino acid content, respectively. The highest yields of oil and protein at 8.24 ± 0.21% and 8.41 ± 0.26% w/w, respectively were obtained from 18 h extraction. Fatty acid analysis of SWP oil was rich in linolenic acid (37.81 ± 0.34%), oleic acid (28.97 ± 0.13%), palmitic acid (21.27 ± 0.05%), stearic acid (6.60 ± 0.09%) and linoleic acid (4.73 ± 0.21%). The clear yellow SWP oil possessed saponification value of 191.51 mg/g, iodine value of 119.37 g I2/g and peroxide value of 2.00 mg equivalent O2/kg. The SWP protein composed of 17 amino acids which aspartic acid, glutamic acid, glycine and serine were the major residues. SDS-PAGE analysis revealed that the SWP protein consisted of distinct protein at around 51, 70, 175 and over 175 kDa. Cytotoxicity of the SWP oil and protein was evaluated by using MTT assay and they showed low cytotoxicity toward keratinocyte cell (HaCat cell line). The SWP oil provided moisturizing effect on pig skin comparable to olive oil, while 1% and 2% of SWP protein showed higher moisturizing efficacy than 3% hydrolyzed collagen. The study indicated that the SWP oil and protein could be potential biomoisturizers for cosmetic products.

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Simultaneous extraction of oil and protein from perilla seed by three-phase partitioning and their application in serum

Cited by 3 publications

References 24 publications

Collagenase and Melanogenesis Inhibitory Effects of Perilla Frutescens Pomace Extract and Its Efficacy in Topical Cosmetic Formulations

Collagenase and Melanogenesis Inhibitory Effects of Perilla Frutescens Pomace Extract and Its Efficacy in Topical Cosmetic Formulations

Green Synthesis Optimization of Glucose Palm Oleate and Its Potential Use as Natural Surfactant in Cosmetic Emulsion

Simultaneous Extraction of Oil and Protein from Silkworm (Bombyx mori L.) Pupae (Lueng Parroj var.) and Their In Vitro Skin Moisturization

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