Simultaneous extraction and rapid visualization of peptidomic and lipidomic body fluids fingerprints using mesoporous aluminosilicate and <scp>MALDI</scp>‐<scp>TOF MS</scp>

Preianò, Mariaimmacolata; Pasqua, Luigi; Gallelli, Luca; Galasso, Olimpio; Gasparini, Giorgio; Savino, Rocco; Terracciano, Rosa

doi:10.1002/pmic.201200204

Cited by 20 publications

(14 citation statements)

References 37 publications

(41 reference statements)

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“…For Western blot, protein extracts were separated on a 12.5% sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes as previously described …”

Section: Methodsmentioning

confidence: 99%

Chronic venous leg ulcers are associated with high levels of metalloproteinases‐9 and neutrophil gelatinase‐associated lipocalin

Serra

Buffone

Falcone

et al. 2013

Wound Repair Regeneration

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Venous ulcers are related to dysfunctions in extracellular matrix. Both matrix metalloproteinases (MMP) and neutrophil gelatinase-associated lipocalin (NGAL) could play a role in the healing process in patients with chronic venous ulcers. We evaluated the role of MMP-9 and NGAL in the healing process in venous ulceration. We performed an open-label, parallel groups, single clinical center study. Patients with chronic venous leg ulcers represented the test group (Group I), whereas patients without chronic ulcers represented the control group (Group II). In Group I plasma and wound fluid samples were collected at the time of admission, at the time of the surgery, and at the follow-up, while ulcer tissues were taken at the time of the surgery. In Group II, plasma and wound fluid were collected at admission and at the time of the surgery, whereas skin tissues were collected at the time of the surgery. Enzyme-linked immunosorbent assay test was used to evaluate the levels of MMP-9 and NGAL in plasma and wound fluid, whereas Western blot analysis was performed to estimate the expression of MMP-9 and NGAL in tissues. Enzyme-linked immunosorbent assay tests revealed significantly higher levels of MMP-9 and NGAL in both plasma and wound fluid of patients with ulcers compared to patients without ulcers (p < 0.01). Moreover, Western blot analysis documented an increased expression of MMP-9 and NGAL in biopsy tissue of patients with ulcers compared to patients without ulcers (p < 0.01). In conclusion MMP-9 and NGAL may correlate with the clinical course of venous ulcers.

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Section: Methodsmentioning

confidence: 99%

Chronic venous leg ulcers are associated with high levels of metalloproteinases‐9 and neutrophil gelatinase‐associated lipocalin

Serra

Buffone

Falcone

et al. 2013

Wound Repair Regeneration

Self Cite

113

119

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“…In both groups, the biopsies obtained at the time of the study were collected for Western blot and then evaluated, as previously described . Immunoblotting was performed using anti‐MMP‐9 and anti‐NGAL monoclonal antibodies and expressed as arbitrary units, as recently described .…”

Section: Methodsmentioning

confidence: 99%

Doxycycline speeds up healing of chronic venous ulcers

Serra

Gallelli

Buffone

et al. 2013

International Wound Journal

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Venous ulcers are common, with an overall prevalence of up to 2% in the general population of western countries, and have significant socioeconomic impact. Matrix metalloproteinases (MMPs) are involved in the alteration of extracellular matrix that could lead to venous ulceration. Sixty-four patients with venous ulcers were recruited in a 22-month period. All patients were subjected to the most appropriate treatment considering also the patient's wishes (compression therapy followed or not by vein surgery). Patients were randomised into two groups of 32 persons in each (groups A and B). Patients of group A in addition to the basic treatment, described above, received the administration of oral low doses of doxycycline 20 mg b.i.d. for 3 months, whereas patients of group B received basic treatment only. Healing was assessed by means of direct ulcer tracing with computerised planimetry. Group A showed a higher healing rate compared with group B. In group B, the lower healing rate was related to higher levels of MMP-9; neutrophil gelatinase-associated lipocalin and vascular endothelial growth factor, documented in plasma; wound fluid and biopsies executed and compared between both groups. Pharmacological treatments, as doxycycline administration, which by means of its immunomodulatory and anti-inflammatory actions, through the inhibition of MMP, could improve extracellular matrix functioning and represent a possible solution to support wound healing.

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“…Besides endogenous peptidome analysis, size-exclusive effect of mesoporous materials was also employed for selective extraction of other LMW species, including glycans [137][138][139] and lipids [80], in the presence of interference HMW proteins. Zou et al [137] prepared oxidized mesoporous carbon for selective enrichment of N-linked glycans after PNGase F cleavage in the presence of residual proteins, and 5 N-linked glycans were identified as potential biomarkers of liver cancer.…”

Section: Imprinted Polymersmentioning

confidence: 99%

Recent advances in applications of nanomaterials for sample preparation

et al. 2016

Talanta

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Simultaneous extraction and rapid visualization of peptidomic and lipidomic body fluids fingerprints using mesoporous aluminosilicate and MALDI‐TOF MS

Cited by 20 publications

References 37 publications

Chronic venous leg ulcers are associated with high levels of metalloproteinases‐9 and neutrophil gelatinase‐associated lipocalin

Chronic venous leg ulcers are associated with high levels of metalloproteinases‐9 and neutrophil gelatinase‐associated lipocalin

Doxycycline speeds up healing of chronic venous ulcers

Recent advances in applications of nanomaterials for sample preparation

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