Simultaneous Expression of T‐Cell and Myeloid Cell Phenotypes in Eight Newly Established HTLV‐I‐positive T‐Cell Lines

Koizumi, Shigeki; Sugiura, Makoto; Zhang, Xian-kui; Yamashiro, Katsushige; Iwanaga, M; Imai, Shosuke; Õsato, Toyoro

doi:10.1111/j.1349-7006.1992.tb02002.x

Cited by 8 publications

(11 citation statements)

References 18 publications

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“…15 The possibility that HTLV-I might have infected the few CD33/TCR␣/␤ double positive cells present in the original CD4…”

Section: Figurementioning

confidence: 99%

“…Coexpression of CD4 and CD8 antigens was observed in adult T cell leukemia (ATL) cells, 11 or ATL-derived cell lines, 12 and in purified CD8 + lymphocytes or peripheral blood mononuclear cells (PBMC) infected in vitro with HTLV-I. 13 Moreover, coexpression of myeloid and T cell markers was detected in ATL cells 14 and in cell lines derived from ATL, 15 or TSP/HAM 16 or from PBMC infected with HTLV-I. 15 In these cases, it was not clear whether the presence of both myeloid and lymphoid phenotype was the result of infection of a pre-existing biphenotypic cell population, 14 or of postinfection virus-dependent phenotypic changes.…”

Section: Introductionmentioning

confidence: 99%

“…13 Moreover, coexpression of myeloid and T cell markers was detected in ATL cells 14 and in cell lines derived from ATL, 15 or TSP/HAM 16 or from PBMC infected with HTLV-I. 15 In these cases, it was not clear whether the presence of both myeloid and lymphoid phenotype was the result of infection of a pre-existing biphenotypic cell population, 14 or of postinfection virus-dependent phenotypic changes. 17 Correspondence: G Graziani, Department of Neuroscience, University of Rome Tor Vergata, Via di Tor Vergata 135, 00133 Rome, Italy; Fax: 39-6 7259 6323 Received 8 January 1998; accepted 28 October 1998 The present study was undertaken to obtain more information about the possible mechanism underlying biphenotypic induction by HTLV-I in human PBMC.…”

Section: Introductionmentioning

confidence: 99%

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In vitro infection of CD4+ T lymphocytes with HTLV-I generates immortalized cell lines coexpressing lymphoid and myeloid cell markers

et al. 1999

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“…15 The possibility that HTLV-I might have infected the few CD33/TCR␣/␤ double positive cells present in the original CD4…”

Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro infection of CD4+ T lymphocytes with HTLV-I generates immortalized cell lines coexpressing lymphoid and myeloid cell markers

et al. 1999

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“…Several hypotheses could explain the cause of this unusual phenotype. The CD4/CD8 double‐positive phenotype is characteristically associated with the majority of immature thymic precursors 9 . During intrathymic T‐cell ontogeny, immature CD4+/CD8+ thymocytes develop into functionally competent CD3+/CD4+/CD8– or CD3+/CD4–/CD8+ T cells after transient expression of the CD4/CD8 double‐positive phenotype 10 .…”

Section: Discussionmentioning

confidence: 99%

CD4/CD8 double-positive, acute type of adult T-cell leukemia/lymphoma with extensive cutaneous involvement

et al. 2006

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A 67‐year‐old Korean man presented with a 2‐week history of pruritic cutaneous lesions on the trunk and a 2‐month history of cervical neck masses. The cutaneous lesions had been spreading rapidly for the last week. The past history included an unknown liver disease about 40 years previously, and he had never been transfused. The physical examination revealed multiple cervical lymph node enlargements and a two‐finger width of splenomegaly with tenderness. Numerous nodules and plaques were distributed on the trunk, face, and proximal extremities (Fig. 1). The results of laboratory tests were as follows: leukocyte count, 24,200/mm3, with lymphocytes 15,560/mm3; serum lactate dehydrogenase, 943 IU/L (normal range, 263–450 IU/L); alkaline phosphatase, 135 IU/L (normal range, 35–60 IU/L); blood urea nitrogen, 32 mg/dL (normal range, 3–24 mg/dL); serum creatinine, 1.7 mg/dL (normal range, 0.3–1.6 mg/dL); serum calcium, 17.5 mg/dL (normal range, 8.8–10.2 mg/dL). Other laboratory results, including liver function test and urine analysis, showed no abnormalities. The examination of peripheral blood revealed multilobulated atypical lymphoid cells. The radiologic images showed hilar, para‐aortic lymph node enlargement and splenomegaly. A skin biopsy from the nodular lesion revealed massive infiltration of small‐ to medium‐sized atypical lymphoid cells in the dermis (Fig. 2a). The infiltrating cells were positive for CD3 (Fig. 2b), CD4 (Fig. 2c), CD5, and CD8 (Fig. 2d), but negative for CD20, CD34, CD56, CD68, and terminal deoxynucleotidyl transferase (TdT) in the immunohistochemical studies on paraffin sections. They also showed high Ki‐67 labeling (80–90%), and this finding reflects their highly proliferative nature. Polymerase chain reaction (PCR) analysis showed a distinct band for the T‐cell γ receptor gene, confirming the T‐cell clonality of the infiltrating neoplastic cells. Specimens from the cervical lymph node and bone marrow showed the same results in immunohistochemical studies. PCR analysis of the DNA from peripheral leukemic cells showed human T‐cell leukemia virus type I (HTLV‐I) proviral integration. The patient was diagnosed as having the acute type of adult T‐cell leukemia/lymphoma (ATLL). The disease course was extremely aggressive, and he died of acute renal failure on the 16th day following diagnosis. 1 Pruritic erythematous nodules and plaques on the face, trunk, and upper arms 2 There is dense infiltration of medium‐ to large‐sized, pleomorphic tumor cells. (a) Hematoxylin and eosin stain, ×400. Immunohistochemical studies demonstrate CD3+ (b), CD4+ (c), and CD8+ (d) tumor cells (all ×200)

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“…The CD4/CD8 double-positive phenotype is characteristically associated with the majority of immature thymic precursors. 9 During intrathymic T-cell ontogeny, immature CD4+/CD8+ thymocytes develop into functionally competent CD3+/CD4+/CD8-or CD3+/CD4-/CD8+ T cells after transient expression of the CD4 /CD8 double-positive phenotype. 10 The unusual expansion of a CD4/CD8 doublepositive cell population may result from clonal expansion of a less differentiated cell population immortalized by HTLV-I infection; however, the finding that terminal deoxynucleotidyl transferase (TdT), which is a marker for thymic T-cell precursors, was not expressed in the infiltrative tumor cells opposes this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

CD4/CD8 double-positive, acute type of adult T-cell leukemia/lymphoma with extensive cutaneous involvement

Kim¹,

Hwang²,

Kim³

et al. 2005

Int J Dermatol

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A 67-year-old Korean man presented with a 2-week history of pruritic cutaneous lesions on the trunk and a 2-month history of cervical neck masses. The cutaneous lesions had been spreading rapidly for the last week. The past history included an unknown liver disease about 40 years previously, and he had never been transfused. The physical examination revealed multiple cervical lymph node enlargements and a two-finger width of splenomegaly with tenderness. Numerous nodules and plaques were distributed on the trunk, face, and proximal extremities (Fig. 1). The results of laboratory tests were as follows: leukocyte count, 24,200/ mm 3 , with lymphocytes 15,560/mm 3 ; serum lactate dehydrogenase, 943 IU / L (normal range, 263 -450 IU/L); alkaline phosphatase, 135 IU/L (normal range, 35 -60 IU/ L); blood urea nitrogen, 32 mg /dL (normal range, 3 -24 mg/dL); serum creatinine, 1.7 mg / dL (normal range, 0.3 -1.6 mg/dL); serum calcium, 17.5 mg/dL (normal range, 8.8 -10.2 mg/dL). Other laboratory results, including liver function test and urine analysis, showed no abnormalities. The examination of peripheral blood revealed multilobulated atypical lymphoid cells. The radiologic images showed hilar, para-aortic lymph node enlargement and splenomegaly. A skin biopsy from the nodular lesion revealed massive infiltration of small-to medium-sized atypical lymphoid cells in the dermis (Fig. 2a). The infiltrating cells were positive for CD3 ( Fig. 2b), CD4 (Fig. 2c), CD5, and CD8 (Fig. 2d), but negative for CD20, CD34, CD56, CD68, and terminal deoxynucleotidyl transferase (TdT) in the immunohistochemical studies on paraffin sections.They also showed high Ki-67 labeling (80 -90%), and this finding reflects their highly proliferative nature. Polymerase chain reaction (PCR) analysis showed a distinct band for the T-cell γ receptor gene, confirming the T-cell clonality of the infiltrating neoplastic cells. Specimens from the cervical lymph node and bone marrow showed the same results in immunohistochemical studies. PCR analysis of the DNA from peripheral leukemic cells showed human T-cell leukemia virus type I (HTLV-I) proviral integration. The patient was diagnosed as having the acute type of adult T-cell leukemia / lymphoma (ATLL). The disease course was extremely aggressive, and he died of acute renal failure on the 16th day following diagnosis.

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Simultaneous Expression of T‐Cell and Myeloid Cell Phenotypes in Eight Newly Established HTLV‐I‐positive T‐Cell Lines

Cited by 8 publications

References 18 publications

In vitro infection of CD4+ T lymphocytes with HTLV-I generates immortalized cell lines coexpressing lymphoid and myeloid cell markers

In vitro infection of CD4+ T lymphocytes with HTLV-I generates immortalized cell lines coexpressing lymphoid and myeloid cell markers

CD4/CD8 double-positive, acute type of adult T-cell leukemia/lymphoma with extensive cutaneous involvement

CD4/CD8 double-positive, acute type of adult T-cell leukemia/lymphoma with extensive cutaneous involvement

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