Simultaneous expression by porcine aorta endothelial cells of glycosphingolipids bearing the major epitope for human xenoreactive antibodies (Gal?1?3Gal), blood group H determinant andN-glycolylneuraminic acid

Bouhours, Danièle; Pourcel, Christine; Bouhours, Jean-François

doi:10.1007/bf01053190

Cited by 106 publications

(75 citation statements)

References 27 publications

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“…Blood group A and H hexaglycosylceramides as well as H pentaglycosylceramides are also abundant constituents (Holgersson et al, 1990b;Bouhours et al, 1997). Human blood group H determinant has also been detected by immunostaining on primary aortic endothelial cells in culture (Bouhours et al, 1996). These findings are in contrast to a study in which immunofluorescent staining of different pig tissue failed to detect blood group determinants in blood vessels .…”

Section: Discussioncontrasting

confidence: 55%

Glycosphingolipid expression in pig aorta: Identification of possible target antigens for human natural antibodies

Hallberg¹,

Holgersson

Samuelsson³

1998

Glycobiology

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Total non-acid glycosphingolipids were isolated from the aortas of more than 80 pigs. The glycolipids were separated by HPLC, analysed by thin-layer chromatography, and tested for reactivity with monoclonal anti-blood group antibodies. The fractions were structurally characterized by NMR spectroscopy and mass spectrometry. Reactivity with both antiblood group A and H antibodies was seen. The major glycosphingolipid constituents were globotriand globotetraosylceramides and blood group H pentaglycosylceramides based on type 1 and type 2 core saccharide chains. Globopentaosylceramides, blood group H hexaglycosylceramides based on type 4 chain, and blood group A hexaglycosylceramides based on type 1 core chain were also present. Two structures, that may be important targets for human antibodies initiating hyperacute rejection following pig to human xenotransplantation, were present as minor constituents compared to the blood group components. These were Galα1,3neolactotetraosylceramide and a Galα1,3Le x structure. A Le b /Y hexaglycosylceramide was also present.

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Section: Discussioncontrasting

confidence: 55%

Glycosphingolipid expression in pig aorta: Identification of possible target antigens for human natural antibodies

Hallberg¹,

Holgersson

Samuelsson³

1998

Glycobiology

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“…This sulfate and glucuronic acid carrying GSL was also reported to be present in rat brain microvessels by the same group (Miyatani et al, 1990), indicating prevalence for brain vascular cells of different species. Primary cultures of porcine endothelial cells revealed major neutral GSLs of the globo-series (Bouhours et al, 1996), thus showing some homology to endothelial cells of human origin (Gillard et al, 1987). However, the overall feature of all endothelial cells analyzed so far lies in the dominance of G M3 .…”

Section: Discussionmentioning

confidence: 99%

Isolation and structural characterization of glycosphingolipids of in vitro propagated bovine aortic endothelial cells

Duvar¹,

Peter‐Katalinić²,

Hanisch

et al. 1997

Glycobiology

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Neutral glycosphingolipids and gangliosides were isolated from 3.7 x 10 9 primary bovine aortic endothelial cells and structurally characterized by immunological and chemical methods. Glucosyl-and lactosylceramide were detected as the main neutral glycosphingolipids (28% and 40% of total orcinol stain, respectively); LcOse 3 Cer and nLcOse 4 Cer were expressed to somewhat minor amounts (16% and 10% of total orcinol stain, respectively), and nLcOse 6 Cer occurred only in trace quantities. No neutral glycosphingolipids of the ganglio-series (GgOse 3 Cer and GgOse 4 Cer) and the globo-series (GbOse 4 Cer and the Forssman antigen) have been detected; only traces of GbOse 3 Cer were identified by TLC immunostaining. Positive CD15 bands obtained by TLC overlay with anti-Gaipi-4(Fucal-3)GlcNAcpi-R antibody indicated the presence of lipid bound Lewis" antigen, whereas the isomeric Lewis 3 structure (Gaipi-3(Fuccd-4)GlcNAcpi-R) was not detectable. G M3 substituted with Neu5Gc and Neu5Ac in a 2:1 ratio was the major ganglioside comprising about 95% within the whole ganglioside fraction. G M3 -structures were further characterized by FAB-MS and GC-MS of the native compounds and their permethylated derivatives. C 18 -sphingosine was the only long chain base, whereas variation occurred due to C^o^a and C 16 fatty acids. Terminally a2-3 sialylated neolacto-series gangliosides with nLcOse 4 -and nLcOse 6 Cer (<5% of total resorcinol stain) were found in almost equal quantities, whereas no a.2-6 sialylated counterparts were detected.

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“…In a previous analysis of sera from the patients transplanted with porcine ICCs, Abs with non-Gal reactivity were detected in some of them (26) (34)(35)(36), has been widely discussed as a potential non-Gal antigen (13,37 (28). The Neu5Aca3Galb3GlcNAcb-structure, also known as the DU-PAN-2 antigen (41,42),…”

Section: Patient 7 Also In This Blood Group a Patient There Was A VImentioning

confidence: 99%

Anticarbohydrate Antibody Repertoires in Patients Transplanted with Fetal Pig Islets Revealed by Glycan Arrays

Blixt

Kumagai‐Braesch

Tibell

et al. 2009

American Journal of Transplantation

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Ten patients with type I diabetes were transplanted with porcine fetal islet-like cell clusters (ICC) between 1990 and 1993. A significant rise in the anti-a -Gal antibody titers was seen posttransplant, but also nona -Gal-specific antibodies were detected in some patients. We have reanalyzed the carbohydrate specificity of antibodies in the sera from seven of these patients taken before transplantation, 1, 6 and 12 months posttransplantation using a glycan array with 200 structurally defined glycans. The main findings were: (i) prepig ICC transplantation patients had antibodies reactive with terminal a -GalNAc (e.g. the Forssman antigen, but not the blood group A determinant in blood group A patients), a -Gal (except blood group B determinants in B individuals), b 3-linked Gal especially Galb 1,3GlcNAc even if terminally sulfated or sialylated, b -GlcNAc except if b 1,3-linked and oligomannosyl compounds; (ii) the titers of all carbohydrate-specific antibodies detected before transplantation rose after transplantation; (iii) the kinetics of the antibody responses differed between patients; (iv) in some patients antibodies reacting with Gala 1,3Lex and several structures terminated with Neu5Gc appeared after transplantation. In conclusion, anti-a -Gal antibodies are the predominant anticarbohydrate antibodies detected after porcine ICC transplantation, with some patients also developing Neu5Gc-specific antibodies. Their clinical significance needs to be established.Key words: Anticarbohydrate antibodies, blood groups, glycan arrays, islet transplantation, xenotransplantation Abbreviations: a 1,3Gal-T, a 1,3-

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Simultaneous expression by porcine aorta endothelial cells of glycosphingolipids bearing the major epitope for human xenoreactive antibodies (Gal?1?3Gal), blood group H determinant andN-glycolylneuraminic acid

Cited by 106 publications

References 27 publications

Glycosphingolipid expression in pig aorta: Identification of possible target antigens for human natural antibodies

Glycosphingolipid expression in pig aorta: Identification of possible target antigens for human natural antibodies

Isolation and structural characterization of glycosphingolipids of in vitro propagated bovine aortic endothelial cells

Anticarbohydrate Antibody Repertoires in Patients Transplanted with Fetal Pig Islets Revealed by Glycan Arrays

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