Simultaneous estimation of recently FDA approved co-formulated ophthalmic solution benoxinate and fluorescein: Application to aqueous humor

Radwan, Aya Saad; Salim, Mohamed M.; Hadad, Ghada M.; Belal, Fathalla; Elkhoudary, Mahmoud M.

doi:10.1016/j.saa.2021.120599

Cited by 4 publications

(5 citation statements)

References 14 publications

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Section: Resultsmentioning

confidence: 99%

“…The results were very reliable with those obtained through the application of the reported methods. 31,34 According to the results of statistical tests such as the analysis of variance (ANOVA), F-test, and Student's t-test, there was not a substantial variation in the analytical efficiency in terms of accuracy and precision and, respectively, as shown in Supplementary Tables 3S and 4S. : second derivative of ratio spectra; RD: ratio spectra difference; ERS: extended ratio subtraction; AS: absorbance subtraction; QA: Q-absorbance ratio; AUC: area under the curve; RSD: relative standard deviation; LOD: limit of detection; LOQ: limit of quantification.…”

Section: Applicationmentioning

confidence: 99%

“…15 Many analytical techniques have been developed to quantify LIG, such as electrochemical methods, 16–21 chromatographic methods, 22–28 spectrophotometric methods, 29–31 and FLS including, spectrofluorimetry method, 32 a chromatographic method, 33 and spectrophotometric method. 34 Meanwhile, no methods have been reported that can simultaneously quantify LIG and FLS in a co-formulated eye solution. Thus, the development of fast, low-cost, and sensitive methods for the simultaneous detection of LIG and FLS is still interesting.…”

Section: Introductionmentioning

confidence: 99%

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A sustainable data processing approach using ultraviolet-spectroscopy as a powerful spectral resolution tool for simultaneously estimating newly approved eye solution in the presence of extremely carcinogenic impurity aided with various greenness and whiteness assessment perspectives: Application to aqueous humor

Attia,

El-Olemy,

Abbas

et al. 2023

Journal of Chemical Research

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Drug impurities are now seen as a major threat to drug safety around the world, especially when it comes to carcinogenic impurities. Here, we present the first spectrophotometric approach for the quantification of lignocaine and fluorescein in the presence of 2,6-xylidine, lignocaine’s carcinogenic impurity. The approach depends on overcoming unresolved bands through a data processing strategy employing 10 affordable, simple, and sensitive spectrophotometric methods. Fluorescein analysis (1–16 μg mL−1) was performed using direct ultraviolet spectrophotometry (D0) at 478 nm; then, the ratio subtraction method allowed the removal of interference caused by the fluorescein spectrum. From the resulting ratio spectra, 2,6-xylidine (40–160 μg mL−1) can be efficiently determined at 280 nm. However, lignocaine (72–320 μg mL−1) was analyzed using different ultraviolet-based methods, including continuous wavelet transform, ratio derivative by Savitzky–Golay filters, mean centering, second derivative of ratio spectra, ratio spectra difference spectrophotometry, extended ratio subtraction, absorbance subtraction, Q-absorbance ratio, and area under the curve. In line with International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH), the presented approach was evaluated by its coefficients of determination, detection limits, quantification limits, and relative standard deviations. Similarly, the developed approach was assessed for whiteness, greenness, and sustainability using five assessment tools, including National Environmental Method Index, Eco-Scale Assessment, Complementary Green Analytical Procedure Index, Analytical Greenness Metric, and RGB12, offering promising results. Owing to the satisfactory analytical performance, besides the sustainability, affordability, simplicity, and cost efficiency of the presented methods, their application for quality control and in situ analysis in minimal-infrastructure laboratories increases, increasing the surveillance potential.

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Section: Resultsmentioning

confidence: 99%

Section: Applicationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A sustainable data processing approach using ultraviolet-spectroscopy as a powerful spectral resolution tool for simultaneously estimating newly approved eye solution in the presence of extremely carcinogenic impurity aided with various greenness and whiteness assessment perspectives: Application to aqueous humor

Attia,

El-Olemy,

Abbas

et al. 2023

Journal of Chemical Research

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“…Difference Spectrophotometry, Q-Absorbance Method [8] Allopurinol and Lesinurad Simultaneous Equation Method [9] Ambroxol, Salbutamol, and Theophylline Simultaneous Equation Method [10] Bromfenac and Ofloxacin Derivative Spectrophotometry [11] Esomeprazole and Naproxen Derivative Spectrophotometry [12] Fluorescein and Benoxinate Simultaneous Equation Method [13] Fluticasone and Formoterol Simultaneous Equation Method, Q-Absorbance Method [14] Furazolidone and Metronidazole Q-Absorbance Method [15] Hydrochlorothiazide and Carvedilol Q-Absorbance Method [16] Ledipasvir and Sofosbuvir Derivative Spectrophotometry [17] Levosulpiride and Rabeprazole sodium Derivative Spectrophotometry [18] Metformin HCl and Anagliptin Q-Absorbance Method [19] Nalidixic acid and Metronidazole Difference Spectrophotometry [20] Pamabrom, Mefenamic Acid, and Dicyclomine Hydrochloride Simultaneous Equation Method [21] Quinfamide and Mebendazole Q-Absorbance Method [22] Sofosbuvir and Velpatasvir Simultaneous Equation Method [23] Sumatriptan and Naproxen Simultaneous Equation Method [24] Telmisartan and Hydrochlorothiazide Q-Absorbance Method [25] Tinidazole and Norfloxacin Difference Spectrophotometry [26] Β-Carotene and Lycopene Simultaneous Equation Method [27] Paracetamol Paracetamol (PCM), widely known as Acetaminophen is an OTC medicine having analgesic and antipyretic properties used in mild to moderate pain and fever. It is chemically N-(4-hydroxyphenyl) acetamide (Figure 5).…”

Section: Acetaminophen and Chlorzoxazonementioning

confidence: 99%

A Review on Methods Developed for Estimation of Paracetamol in Combination with Other Drugs

Baghel¹,

Shah²

2023

Int J Pharm Res Allied Sci

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In this ever-growing world, it is crucial to improve upon the formulations in terms of potency, patient acceptability, fewer side effects, and quicker relief. Due to these requirements, the market is flooded with various combination dosage forms, with a constant increase in number. Paracetamol is a commonly used non-steroidal anti-inflammatory drug (NSAID) that has antipyretic and analgesic action. This drug is available in a wide range of combinations. It acts by inhibiting the production of prostaglandins, which combat pain and inflammation. A simultaneous multicomponent analysis is used to determine the estimation of medicines that are available in combination. Different analytical techniques are available for their determination, one of which includes the use of UV spectrophotometric methods. This review focuses on a variety of paracetamol combinations with drugs like Domperidone, Aceclofenac, Diclofenac Sodium, Etodolac, Ibuprofen, Piroxicam, Caffeine, Aspirin, and their simultaneous estimation by different UV methods viz. Simultaneous equation method, Absorbance ratio (Q-Analysis), Difference spectrophotometry, Derivative spectroscopy method, and a few other chemometric methods. This manuscript would provide the platform to have exhaustive literature on methods used for the estimation of paracetamol with different drugs using a spectrophotometer. It would help the researchers and scholars who are working in the area.

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“…Only few number of analytical methods has been reported for concomitant assay of BNX HCl and FLR sodium including UV spectrophotometric methods [ 21 ] , and chromatographic methods [ 22 , 23 ] . However, both reported chromatographic methods involve the use of acetonitrile as organic modifier which cause harm to health and environment.…”

Section: Introductionmentioning

confidence: 99%

Quality by design paradigm for optimization of green stability indicating HPLC method for concomitant determination of fluorescein and benoxinate

Kamal

Habib

Hammad

et al. 2023

Sci Rep

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A green, robust and fast stability indicating chromatographic method has been developed for concomitant analysis of fluorescein sodium and benoxinate hydrochloride in the presence of their degradation products within four minutes. Two different designs including fractional factorial and Box–Behnken designs were implemented for screening and optimization steps, respectively. The optimum chromatographic analysis was achieved using a mixture of isopropanol and 20 mM potassium dihydrogen phosphate solution (pH 3.0) in the ratio 27:73 as mobile phase. The flow rate was 1.5 mL/min and column oven temperature was 40 °C. Chromatographic analysis was performed on Eclipse plus C18 (100 mm × 4.6 mm × 3.5 μm) column with DAD detector set at 220 nm. A linear response was acquired over the range of 2.5–60 μg/mL and 1–50 μg/mL for benoxinate and fluorescein respectively. Stress degradation studies were executed under acidic, basic, and oxidative stress conditions. The method was implemented for quantitation of cited drugs in ophthalmic solution with mean percent recovery ± SD of 99.21 ± 0.74 and 99.88 ± 0.58 for benoxinate and fluorescein respectively. The proposed method is more rapid and eco-friendly compared to the reported chromatographic methods for determination of cited drugs.

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Simultaneous estimation of recently FDA approved co-formulated ophthalmic solution benoxinate and fluorescein: Application to aqueous humor

Cited by 4 publications

References 14 publications

A Review on Methods Developed for Estimation of Paracetamol in Combination with Other Drugs

Quality by design paradigm for optimization of green stability indicating HPLC method for concomitant determination of fluorescein and benoxinate

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