Simultaneous Estimation of Ramipril and Amlodipine in Bulk and tablet Dosage form by RP-HPLC Method

Rajput,

doi:10.7324/japs.2012.2724

Cited by 8 publications

(4 citation statements)

References 2 publications

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“…Linearity was studied in the range of concentrations of 10-50 μg/ml of amlodipine and 200-1000 μg/ml of perindopril. The retention time for amlodipine and perindopril was 8.51 and 5.28 min, respectively [56][57][58][59][60][61][62][63][64]. buffer solution (pH 5.33) (10:42.08:47.92, V/V/V), mobile phase rate-1.12 ml/min [23,24].…”

Section: Fig 1: the Chemical Structures Of Amlodipinementioning

confidence: 99%

Analysis of Approaches to the Development and Validation of the Methods of Analysis of Some Active Pharmaceutical Ingredients From the Group of Calcium Channel Blockers in Drugs and Biological Liquids

Logoyda

2019

Int J App Pharm

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Calcium channel blockers prevent calcium from entering cells of the heart and blood vessel walls, resulting in lower blood pressure. Calcium channel blockers, also called calcium antagonists, relax and widen blood vessels by affecting the muscle cells in the arterial walls. Physico-chemical analysis methods are increasingly being introduced into fundamental pharmaceutical research and pharmaceutical analysis practice, taking into account their high sensitivity, accuracy, specificity and expressiveness. Analytical method development is increasingly being introduced into fundamental pharmaceutical research and pharmaceutical analysis practice, taking into account their high sensitivity, accuracy, specificity and expressiveness. Search criteria was analytical method development for medicines from group of calcium channel blockers. Literature survey has been done in range of years 1990-2018 to make the review updated and comprehensive and to show the new approacheches to the development of the methods of analysis of calcium channel blockers. The sources were world recognized journals and key words used as filter were calcium channel blockers, amlodipine, nifedipine, verapamil, validation, method development, spectrophotometry, HPLC, UHPLC. Chromatographic methods of analysis amongst others have the greatest specificity and objectivity and allow for qualitative and quantitative determination of API in combinated dosage forms and biological fluids without prior separation of the components. We can conclude that analysts are constantly working on developing new methods of analysis and their optimization in order to save time and consumables, which also ensures the efficiency of the developed method. The main disadvantage of the described methods of API analysis can be considered long term from the beginning of chromatography to API release and specific solvents used as the mobile phase in HPLC. It is necessary to develop methods and to select such chromatographic conditions that will provide high speed and high efficiency at lower pressure of the system. This reduces the amount of used mobile phase, which reduces cost analysis accordingly, while at the same time providing the necessary specificity, accuracy and reproducibility of the results of the analysis during quality control. Also, the reduction of analysis time is achieved by simplifying the conditions for sample preparation.

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Section: Fig 1: the Chemical Structures Of Amlodipinementioning

confidence: 99%

Analysis of Approaches to the Development and Validation of the Methods of Analysis of Some Active Pharmaceutical Ingredients From the Group of Calcium Channel Blockers in Drugs and Biological Liquids

Logoyda

2019

Int J App Pharm

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“…Nowadays, chromatographic methods are the undisputed leaders in the analysis of dosage forms. The analysis of dosage forms of ramipril is no exception, where liquid chromatography techniques (HPLC) are widely used for the purposes of routine pharmaceutical analysis [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]. European Pharmacopoeia (Ph.…”

Section: Introductionmentioning

confidence: 99%

Two masterful alternative spectrophotometric methods for the determination of ramipril in tablets

Typlynska,

Horyn,

Kucher

et al. 2024

ScienceRise: Pharmaceutical Science

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The aim of the work was to develop two simple, rapid, economic, alternative spectrophotometric methods for the determination of ramipril in tablets based on the reaction with sulfonephthalein dyes (bromphenol blue (BPB) and cresol red (CR)). Materials and methods. Analytical instrumentation: Shimadzu UV-1800 double beam UV-VIS spectrophotometer (Japan) with attached UV-Probe ver. 2.62 software, RAD WAG AS 200/C precise analytical balance (Poland). Ramipril (purity ≥98 % (HPLC)) was purchased from AARTI Industries Limited (India). Ramipril tablets 5 mg and 10 mg were purchased from a local pharmacy. Results and discussion. Two spectrophotometric methods for the determination of ramipril in tablets have been developed. We have tested various sulfophthalein dyes (BPB, bromocresol green, bromthymol blue, thymol blue, CR) in order to choose the optimal for the method development. According to the results of the experimental studies, we chose BPB and CR as reagents, and the solvent was acetonitrile for both methods. The optimal conditions for the quantitative determination of ramipril in tabletsy using BPB were established: dye concentration - 2.35×10-4 mol/L, volume of BPB solution – 1.0 ml, without heating, wavelength – 598 nm, reaction time – 5 min, temperature of the solution – 25 °C. The optimal conditions for the quantitative determination of ramipril in tabletsusing CR were established: dye 1.33×10-4 mol/L, volume of CR solution – 1.0 ml, without heating, wavelength – 395 nm, reaction time – 5 min, temperature of the solution – 25 °C. The spectrophotometric method by using BPB was linear in the concentration range of 1.99-5.96 µg/mL, LOD – 0.20 µg/mL, LOQ – 0.60 µg/mL. The spectrophotometric method using CR was linear in the concentration range of 0.42-5.44 µg/mL, LOD – 0.10 µg/mL, and LOQ – 0.36 µg/mL. The results of the study on robustness, accuracy, and precision were within the acceptance criteria. The results of studying the «greenness» of both methods indicate an excellent «green» analysis.Conclusions. Developed methods can be used as an alternative method for the routine analysis of ramipril tablets

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“…The extensive literature review revealed that various LC methods have been published for the estimation of Amlodipine besylate [5][6][7][8][9][10][11][12][13], Rosuvastatin calcium [14][15][16] and Fimasartan potassium trihydrate [17] in combination.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous estimation of amlodipine besylate, Rosuvastatin calcium and Fimasartan potassium trihydrate combination used in the treatment of hypertension using LC method

et al. 2020

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The proposed sensitive, precise, and accurate reverse phase-liquid chromatography (RP-LC) method is used for simultaneous estimation Amlodipine besylate, Rosuvastatin calcium and Fimasartan potassium trihydrate in bulk and synthetic mixture which is under phase 3 trial. An accurate and cost effective isocratic approach is employed by using Phenomenex C-18 column (250 mm × 4.6 mm, 5 μm). A method was developed and validated by focusing on ICH Q2 (R1) guideline parameters, efficient separation of all three drugs were obtained by optimised solvent mixture of acetonitrile and 0.02 M potassium dihydrogen phosphate buffer (49:51, v/v) pH 3.0 adjusted with 1% O-phosphoric acid used as mobile phase, flow rate was maintained 1 ml/min and analysis was carried out by using PDA detector at common wavelength 242 nm. The linearity ranges were found to be 1-7 μg/mL for Amlodipine besylate with correlation coefficient (r 2 ) 0.9966, 2-12 μg/ mL for Rosuvastatin calcium with correlation coefficient (r 2 ) 0.9998 and 6-36 μg/mL for Fimasartan potassium trihydrate with correlation coefficient (r 2 ) 0.9990, respectively. The proposed LC method has potential qualitative as well as quantitative applications for simultaneous estimation of Amlodipine besylate, Rosuvastatin calcium and Fimasartan potassium trihydrate in bulk and synthetic mixture.

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Simultaneous Estimation of Ramipril and Amlodipine in Bulk and tablet Dosage form by RP-HPLC Method

Cited by 8 publications

References 2 publications

Analysis of Approaches to the Development and Validation of the Methods of Analysis of Some Active Pharmaceutical Ingredients From the Group of Calcium Channel Blockers in Drugs and Biological Liquids

Analysis of Approaches to the Development and Validation of the Methods of Analysis of Some Active Pharmaceutical Ingredients From the Group of Calcium Channel Blockers in Drugs and Biological Liquids

Two masterful alternative spectrophotometric methods for the determination of ramipril in tablets

Simultaneous estimation of amlodipine besylate, Rosuvastatin calcium and Fimasartan potassium trihydrate combination used in the treatment of hypertension using LC method

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