Simultaneous estimation of Esomeprazole and Tadalafil in pharmaceutical formulations using High Performance Liquid Chromatography

Abstract

An easily, specific, precise, and accurate reversed-phase HPLC method was developed and validated for simultaneous estimation of esomeprazole ( Nexium ® ) and tadalafil (Cialis ® ) in pharmaceutical formulation. The separation was achieved by using Hypersil BDS C18 column (250 mm × 4.6 mm; 5.0 μm) and acetonitrile: 0.05 M potassium dihydrogen phosphate buffer at pH 6 adjusted with phosphoric acid as a mobile phase at a flow rate of 1 mL/min. Detection was carried out at wavelength 285nm. The retention time of esomeprazole and tadalafil were 3.1, 3.7 min, respectively. The linearity was established over the concentration ranges of 60-180μg/mL and 40-120μg/mL with correlation coefficients 0.9998 and 0.9996 for esomeprazole and tadalafil, respectively. The mean recoveries were found to be in the ranges of 98-102% for esomeprazole and tadalafil. The proposed method has been validated as per ICH guidelines and successfully applied to the simultaneous estimation of esomeprazole and tadalafil in pharmaceutical formulation.

Environmentally benign first derivative synchronous spectrofluorimetry for the analysis of two binary mixtures containing duloxetine with avanafil or tadalafil in spiked plasma samples

“…It was found that our method is advantageous in various points. Regarding sensitivity, the developed approaches are more sensitive than the published methods [18–20, 22, 38, 39, 41, 46–49]. Furthermore, our method is the simplest and greenest approach for the analysis of the examined drugs in plasma samples.…”

“…Its IUPAC name is (1,3-benzodioxol-5-yl)-2-methyl-2,3,6,7,12,12a-hexahydropyrazino[1 0 ,2 0 :1,6]-pyrido [3,4] indole-1,4-dione [6]. A literature survey displayed many analytical methods for TAD assay such as chromatographic methods [44][45][46], spectrophotometry [47][48][49][50], and spectrofluorimetry [42,51].…”