Simultaneous Electrochemical Determination of Nicotinamide Adenine Dinucleotide and Ascorbic Acid at Carbon Nanotube Electrode

Abstract

Simultaneous electrochemical determination of nicotinamide adenine dinucleotide (NADH) and ascorbic acid (AA) at a carbon nanotube electrode is presented. The discrimination of NADH and AA is conducted with the difference of peak potential by differential pulse voltammetry. Two well-distinguished anodic peaks, +0.56 and +0.26 V, due to NADH and AA are observed. The characteristics of those peaks were independent from each other. The attained characteristics for simultaneous determination of NADH and AA are (i) NADH measurement at the concentration range of 0.030 - 2.0 mM in the presence of 1.2 mM AA, and (ii) AA measurement at the concentration range of 0.030 - 2.0 mM in the presence of 2.0 mM NADH.

Carbon Nanomaterials in Analytical Chemistry

“…These nanomaterials have high electroconductivity, special surface properties, unique electric characteristics, and high potential for surface modifications. These nanomaterials are thus employed for (1) enlarging the electrode surface area, (2) accelerating target binding, and (3) facilitating electron transfer.…”

“…Inoue et al reported on the "Simultaneous Electrochemical Determination of Nicotinamide Adenine Dinucleotide from Ascorbic Acid at a Carbon Nanotube Electrode" in which the discrimination of nicotinamide adenine dinucleotide and ascorbic acid was conducted with some difference in the peak potential. 1 Sipa et al proposed "Electrodes Modified with Composite Layers Based on Poly(3,4-ethylenedioxythiophene) as Sensors for Paracetamol". 2 Composites of the conducting polymer and carbon nanotubes facilitated paracetamol adsorption on the composite polymermodified electrode surface, and realized adsorptive stripping differential pulse voltammetry.…”