Simultaneous dual-colour tracking lipid droplets and lysosomes dynamics using a fluorescent probe

Zheng, Xujun; Zhu, Wencheng; Ni, Fan; Ai, Hua; Gong, Shaolong; Zhou, Xiang; Sessler, JonathanÂ L.; Yang, Chuluo

doi:10.1039/c8sc04462g

Cited by 155 publications

(122 citation statements)

References 75 publications

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“…67,68 The spectroscopic features of TPAn inside the living cells were probed through the fluorescence lifetime imaging (FLIM) using a time-resolved confocal microscope. A punctate dot-like pattern in the cytoplasm (ex = 405 nm, em = 435-800 nm), [69][70][71][72] found to be 9 ms (Figure 8a), which is 90 times higher than that in THF. The results suggested the restriction of translational diffusion of TPAn after the internalization in LDs.…”

Section: Resultsmentioning

confidence: 91%

Deciphering Molecular Self-Assembly through Electron Microscopy and Fluorescence Correlation Spectroscopy

Kundu¹,

Chowdhury²,

Nandi³

et al. 2020

Preprint

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Supramolecular self-assembly of small organic molecules has emerged as a powerful tool to construct well-defined micro- and nanoarchitecture through fine-tuning a range of intermolecular interactions. The size, shape, and optical properties of these nanostructures largely depend on the temperature and polarity of the medium, along with the specific self-assembled pattern of molecular building units. The engineering of supramolecular self-assembled nanostructures with morphology-dependent tunable emission is in high demand due to the promising scope in nanodevices and molecular machines. However, challenges are probing the evolution of molecular aggregates from a true solution and directing the self-assembly process in a pre-defined fashion. The structure of molecular aggregates in the solution can be predicted from fluorescence correlation spectroscopy (FCS) and dynamic light scattering (DLS) analysis. On the other hand, the morphology of the aggregates can also be visualized through electron microscopy. Nevertheless, a direct correlation between emission from molecular aggregates in the aqueous dispersion and their morphology obtained through a solid-state characterization is missing. In the present study, we decipher the sequential evolution of molecular nanofibers from solution to spherical and oblong-shaped nanoparticles through the variation of solvent polarity, adjusting the <a>hydrophobic-hydrophilic interactions</a>. The intriguing case of molecular self-assembly is elucidated employing a newly designed π-conjugated thiophene derivative (TPAn) through a combination of steady-state absorption, emission measurements, FCS, and electron microscopy. The FCS analysis and microscopy results infer that small-sized nanofibers in the dispersion are further agglomerated, resulting in a network of nanofibers upon solvent evaporation. <a>The evolution of organic nanofibers and subtle control over the self-assembly process demonstrated in the current investigation provides a general paradigm to correlate the size, shape, and emission properties of diverse fluorescent molecular aggregates in complex heterogeneous media, including a human cell. </a>

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Section: Resultsmentioning

confidence: 91%

Deciphering Molecular Self-Assembly through Electron Microscopy and Fluorescence Correlation Spectroscopy

Kundu¹,

Chowdhury²,

Nandi³

et al. 2020

Preprint

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“…The only way to observe intracellular polarity is to use optical imaging; therefore, various uorescent probes have been developed to detect the polarity of microenvironments. [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] Most reported polarity probes are based on intramolecular charge transfer (ICT) and show shis in emission wavelengths based on changes in the polarity of a cell's surroundings. However, most of these probes are solvatochromic and have disadvantages including the fact that the uorescence efficiency decreases sharply as the solvent polarity increases, limiting the detection range in hydrophobic environments.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, a polarity sensitive probe for detecting lysosomes and lipid droplets was reported. 27 Even though the probe showed good selectivity, its applications are limited because two different excitation wavelengths must be used to observe each organelle, making it difficult to show the polarity distribution in a cell in real time and thus to quantify the polarity of the organelles. To maintain and regulate appropriate cellular activities, multiple organelles exchange materials and transmit intracellular signals.…”

Section: Introductionmentioning

confidence: 99%

Combining hydrophilic and hydrophobic environment sensitive dyes to detect a wide range of cellular polarity

Park

Juvekar

et al. 2020

Chem. Sci.

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“…The various roles of LDs in cells have been increasingly investigated such as hepatitis C virus production 15 , and early embryonic 16 and cancer development 17 . Recently fluorescent probes 18–20 other than Nile Red as well as BODIPY 493/503 21 for staining cellular LDs have been developed as an adipose assay tool. These probes exhibited efficient emission and selective fluorescence signals for intracellular LDs; however, multistep synthesis was required to construct the framework.…”

Section: Introductionmentioning

confidence: 99%

Selective synthesis of substituted amino-quinoline derivatives by C-H activation and fluorescence evaluation of their lipophilicity-responsive properties

Fuchi

Sakuma

Ohyama

et al. 2019

Sci Rep

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Push-pull type fluorescent amino-quinoline derivatives (TFMAQ) bearing phenyl aromatic groups in the 8-position (TFMAQ-8Ar series) were synthesized via palladium-catalyzed C-H activation reaction in short steps. The N-arylation or C-H activation reactions were selectively controlled with high yield by combinations of palladium and phosphine ligands. The TFMAQ-8Ar analogues exhibited fluorescent solvatochromism in non-polar and polar solvents. In non-polar solvent, the absolute fluorescence quantum yield was high, wheareas the fluorescence was almost quenched in polar solvent. The TFMAQ-8Ar derivatives also showed high fluorescence emission at solid state owing to the planar structure between the quinoline ring and phenyl ring at the 7-amino group, as demonstrated by X-ray crystal structure analysis. The fluorescence imaging of 3T3-L1 cell using TFMAQ-8Ar derivatives was performed by confocal laser microscopy. Strong and specific emissions at lipid droplets were observed owing to the accumulation of TFMAQ-8Ar derivatives. Therefore, we propose that the TFMAQ-8Ar derivatives should become a versatile fluorescence probe for the live imaging of lipid droplets.

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Simultaneous dual-colour tracking lipid droplets and lysosomes dynamics using a fluorescent probe

Abstract: NIM-7 is demonstrated to be a multicomponent fluorescent probe that accumulates in both lipid droplets and lysosomes after entering cells, giving rise to yellow and red fluorescence emission, respectively. This allows the two organelles to be visualized concurrently.

Cited by 155 publications

References 75 publications

Deciphering Molecular Self-Assembly through Electron Microscopy and Fluorescence Correlation Spectroscopy

Deciphering Molecular Self-Assembly through Electron Microscopy and Fluorescence Correlation Spectroscopy

Combining hydrophilic and hydrophobic environment sensitive dyes to detect a wide range of cellular polarity

Selective synthesis of substituted amino-quinoline derivatives by C-H activation and fluorescence evaluation of their lipophilicity-responsive properties

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