Simultaneous DNA Typing of Human Platelet Antigens 2, 3 and 4 by an Allele‐Specific PCR Method

Tanaka, Shigenori; Taniue, Atsuko; Nagao, Nobuo; Ohnoki, Shiro; Shibata, Hirotoshi; Okubo, Yasuto; Yamaguchi, Hideo

doi:10.1111/j.1423-0410.1995.tb02577.x

Cited by 42 publications

(6 citation statements)

References 29 publications

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“…This method is rapid, cost-effective, and suitable in large-scale platelet antigen genotyping, either for NAIT or in genetic population studies. 4,6,9,10,12,14,17 Furthermore, the distribution of HPA-1 to 6 phenotypes in Thai blood donors living in Bangkok is consistent with those found in Northeastern Thailand. 6,7 A previous study showed that the most common HPA antibodies in thrombocytopenic Thai patients were anti-HPA-5b, -HPA-2b, -HPA-3a, and unidentified antibodies.…”

Section: Discussionsupporting

confidence: 80%

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Gene frequencies of the HPA-1 to 6 and Gov human platelet antigens in Thai blood donors

et al. 2005

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Human platelet alloantigens (HPA) are important in neonatal alloimmune thrombocytopenia (NAIT), posttransfusion purpura (PTP), platelet transfusion refractoriness, passive alloimmune thrombocytopenia, and transplantation-associated alloimmune thrombocytopenia. Thus, HPA genotyping is essential in diagnosis and treatment. We analyzed HPA-1 to 6 and Gov alleles, using PCR with sequence specific primers (PCR-SSP) in 500 Thai blood donors who had been HLA class I antigen typed. HPA-4a was present in all samples. HPA-1b, -2b, -5b, and -6b were rare, and HPA-4b was not found. HPA-3a and -3b showed frequencies of 56.0 percent and 44.0 percent, respectively. Gov a and Gov b showed frequencies of 49.1 percent and 50.9 percent, respectively. The prevalence rates of HPA-1 to 6 gene frequencies (GFs) were consistent with those of other Asian populations rather than those of Caucasians. We also report on the GFs of Gov a and Gov b , which also are comparable to those of Asian populations. Our results could establish a useful HPA-and HLA-matched plateletpheresis donor file and provide an improvement of platelet alloantibody detection in alloimmune thrombocytopenic patients, and, therefore, a more effective platelet transfusion program.

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Section: Discussionsupporting

confidence: 80%

“…The PCR-SSP technique has been shown to be a simpler and more reliable method for several HPA genotypes. [9][10][11][12][13][14] This study analyzed HPA-1 to 6 and Gov alleles, using PCR-SSP in Thai blood donors who had been HLA class I antigen typed to establish HPA-genotyped panels for identification of HPA antibodies in routine testing.…”

mentioning

confidence: 99%

Gene frequencies of the HPA-1 to 6 and Gov human platelet antigens in Thai blood donors

et al. 2005

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“…HPA-1, -2, -3, -4, -5, and -6 genotypes were determined by PCR-SSP method as previously described. 1,2 In brief, the PCR reaction mixture (25 µL) contained 1X PCR buffer, 1.0 U of Taq DNA polymerase, 200 µmol/L of each dNTP, 1.8 mmol/L MgCl 2 , 0.5 µmol/L of each specific primer (Table 1), 0.2 µmol/L of internal control primer (to amplify human growth hormone gene), and 50 to 100 ng genomic DNA. PCR amplification was performed with initial denaturing at 95°C for 5 minutes followed by 30 cycles of 30 seconds at 95°C, 1 minute at 58°C (67°C for HPA-2, 63°C for HPA-3, 57°C for HPA-5 and HPA-6), and 45 seconds at 72°C plus a final extension at 72°C for 10 minutes.…”

Section: Hpa Genotypingmentioning

confidence: 99%

Human platelet alloantigen systems in three Chinese ethnic populations

Yan

Zhu

et al. 2006

Immunohematology

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Knowledge of the prevalence of human platelet antigens (HPA) in different populations is important for effective diagnosis and management of immune-mediated platelet disorders. The purpose of this study was to determine HPA gene frequencies in the majority Han ethnic population of China and in ethnic She and Tajik minority populations. Using PCR sequence specific primers, HPA-1, -2, -3, -4, -5, and -6, we determined genotypes for ethnic Han, She, and Tajik blood donors. HPA gene frequencies for Chinese Han were found to be similar to those of She, reflecting the historic affinities of these two populations. HPA gene frequencies for Tajik were closer to those for Caucasians than to Chinese Han, She, or other Asian populations, reflecting their disparate origin and historic geographic isolation. HPA gene frequencies in these Chinese populations reflect their historic origins. Knowledge of these findings may be used to better understand and treat immune-mediated platelet disorders in these populations. Immunohematology 2006;22:6–10.

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“…21 In our HPA-4 PCR-RFLP method, the presence of a mismatched T at the 3′ terminus of the modified primer is efficiently incorporated into the PCR product. 21 PCR-SSP, another DNA method used for platelet antigen typing, 14,22,23 also involves the use of modified PCR primers to distinguish HPA alleles. 24 In PCR-SSP, the PCR primer modifications are used to selectively amplify one HPA allele versus the other.…”

Section: Hpa-4 Genotyping Of Caucasian and North American Indian Populationsmentioning

confidence: 99%

A modified PCR-RFLP genotyping method demonstrates the presence of the HPA-4b platelet alloantigen in a North American Indian population

Reiner¹,

Teramura²

1997

Immunohematology

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The diallelic HPA-4 (Pen/Yuk) platelet alloantigen system is polymorphic in Asian populations and accounts for the majority of cases of neonatal alloimmune thrombocytopenia in Japan. At the molecular level, the HPA-4a/4b dimorphism is associated with an arginine/ glutamine substitution at amino acid 143 of the gene encoding platelet glycoprotein IIIa. Unlike the five other major diallelic human platelet antigen systems (HPA-1, -2, -3, -5, and -6), the nucleotide substitution corresponding to the HPA-4 antigen system does not involve a common naturally occurring restriction enzyme site. This paper describes a new genotyping method for HPA-4 (polymerase chain reaction–restriction fragment length polymorphism [PCR-RFLP]) that involves restriction enzyme digestion of PCR-amplified genomic DNA using a modified PCR primer to create an artificial Taq I restriction site that is present in the HPA-4a but not in the HPA-4b DNA sequence. The HPA-4 PCR-RFLP method was validated by testing a reference panel of 10 known HPA-4 genotyped Japanese individuals. Thus, genotyping by PCR-RFLP can now be performed for all six major HPA systems. Using the HPA-4 PCR-RFLP genotyping method, we determined a frequency of 2.9 percent for the HPA-4b allele in a North American Indian population. This finding indicates the importance of the HPA-4 antigen system as a potential cause of alloimmune thrombocytopenia in American Indians.

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Simultaneous DNA Typing of Human Platelet Antigens 2, 3 and 4 by an Allele‐Specific PCR Method

Cited by 42 publications

References 29 publications

Gene frequencies of the HPA-1 to 6 and Gov human platelet antigens in Thai blood donors

Gene frequencies of the HPA-1 to 6 and Gov human platelet antigens in Thai blood donors

Human platelet alloantigen systems in three Chinese ethnic populations

A modified PCR-RFLP genotyping method demonstrates the presence of the HPA-4b platelet alloantigen in a North American Indian population

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