Simultaneous determination of α-tocopherol and retinol in plasma or red cells by high pressure liquid chromatography

Bieri, John G.; Tolliver, Teresa J.; Catignani, George L.

doi:10.1093/ajcn/32.10.2143

Cited by 949 publications

(331 citation statements)

References 12 publications

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“…Serum a-tocopherol A modification of the method of Bieri et al (1979) was used to extract a-tocopherol from serum samples. In a 2-ml screw cap micro tube (ref.…”

Section: Milkingmentioning

confidence: 99%

The effects of cobalt and iodine supplementation of the pregnant ewe diet on immunoglobulin G, vitamin E, T3 and T4 levels in the progeny

Boland

Hayes

Sweeney

et al. 2008

Animal

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Sixty twin-bearing ewes were allocated to one of four dietary treatments investigating the effects of supplementary iodine or cobalt during late pregnancy on lamb serum immunoglobulin G (IgG), triiodothyronine (T 3 ), thyroxine (T 4 ) and vitamin E concentrations, and lamb IgG absorption efficiency. Ewes were offered grass silage ad libitum supplemented with 800 g per ewe per day of a 190 g/kg crude protein (CP) concentrate from day 126 of gestation until parturition plus one of the following supplements (n 5 15 per treatment); no supplement (C); 26.6 mg iodine per day for final 3 weeks pre partum (I-3); 26.6 mg iodine/day for final week pre partum (I-1); 20 mg cobalt/day for final 3 weeks pre partum (Co-3). Lambs were blood sampled at 24 and 72 h post partum for serum IgG and vitamin E concentrations. Ten lambs from C and I-3 were blood sampled at 1 h post partum for serum IgG, vitamin E, T 3 and T 4 concentrations. There were no differences in serum IgG, vitamin E or T 4 values (P . 0.05) at 1 h post partum between lambs born to the C and I-3 ewes. T 3 levels were lower in I-3 compared with C progeny (P , 0.05). Supplemental iodine reduced colostral IgG absorption efficiency (P , 0.001) and lamb serum IgG concentrations at 24 and 72 h post partum (P , 0.001). Serum vitamin E concentration in I-3 and I-1 lambs was lower than in Co-3 lambs at 24 h post partum, while at 72 h post partum I-3, I-1 and Co-3 lambs had significantly lower concentrations than C lambs (P , 0.001). Supplementing the ewe's diet with 26.6 mg/day of iodine for the final week of pregnancy reduced lamb serum IgG concentration at 24 and 72 h post partum. The lower total and free T 3 values in the progeny of I-3-treated ewes suggest interference in the synthesis and metabolism of thyroid hormones when ewes receive excessive dietary iodine for 3 weeks immediately pre partum. Based on these findings, the indications are that the toxicity level for iodine in the diet of the pregnant ewe should be lowered to 20 mg per ewe per day, equivalent to 40% of its current level. The finding that high-level cobalt supplementation during the final 3 weeks of pregnancy will have a negative effect on serum vitamin E concentration at 72 h post partum is a new and significant finding and previously has not been reported in the literature.

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“…Serum a-tocopherol A modification of the method of Bieri et al (1979) was used to extract a-tocopherol from serum samples. In a 2-ml screw cap micro tube (ref.…”

Section: Milkingmentioning

confidence: 99%

The effects of cobalt and iodine supplementation of the pregnant ewe diet on immunoglobulin G, vitamin E, T3 and T4 levels in the progeny

Boland

Hayes

Sweeney

et al. 2008

Animal

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“…The remainder of the blood samples was centrifuged and aliquoted in the field office before being transported to INCAP in cold boxes for subsequent storage at À171C and shipment to the US on dry ice for analysis at UC Davis. Serum ferritin was determined by enzyme immunoassay (Spectro Ferritin, Ramco Laboratories, Inc., Houston, TX, USA); and retinol and vitamin E were measured by reversephase high-pressure liquid chromatography (Bieri et al, 1979), using a Shimadzu Class VP HPLC (Shimadzu, Columbia, MD, USA) equipped with a photo-diode array detector. Aliquots of plasma for trace element analysis were stored frozen in mineral-free, colorless plastic vials.…”

Section: Dietary Assessmentmentioning

confidence: 99%

Effects of bovine serum concentrate, with or without supplemental micronutrients, on the growth, morbidity, and micronutrient status of young children in a low-income, peri-urban Guatemalan community

Bégin

et al. 2007

Eur J Clin Nutr

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Objective: To determine the effects of dietary supplements containing bovine serum concentrate (BSC, a source of immunoglobulins) and/or multiple micronutrients (MMN) on children's growth velocity, rates of common infections, and MN status. Design: Randomized, controlled, community-based intervention trial. Setting: Low-income, peri-urban Guatemalan community. Subjects: Children aged 6-7 months initially. Interventions: Children received one of four maize-based dietary supplements daily for 8 months, containing: (1) BSC, (2) whey protein concentrate (WPC, control group), (3) WPC þ MMN, or (4) BSC þ MMN. Results: There were no significant differences in growth or rates of morbidity by treatment group. Children who received MMN had lower rates of anemia and (in the group that received WPC þ MMN) less of a decline in serum ferritin than those who did not, but there were no differences in other biochemical indicators of MN status by treatment group. Conclusions: MMN supplementation reduced anemia and iron deficiency in this population, but the MMN content and source of protein in the supplements did not affect other indicators of MN status, growth or morbidity. Sponsorship: Funding was provided by LG Laboratories (Ames, Iowa), which produces the bovine serum concentrate used in this study. The members of the research team have no financial involvement in this company.

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“…These samples were subsequently defrosted and vitamin C, vitamin E and copper levels measured in plasma, and selenium in serum. Copper (Varley et al, 1980) and selenium (Burtis & Ashwood, 1986) were assayed using atomic absorption spectroscopy, vitamin E using high-pressure liquid chromatography with UV detection (Bieri et al, 1979), and vitamin C by a spectrophotometric assay (Burtis & Ashwood, 1986).…”

Section: Data Collectionmentioning

confidence: 99%

Lung function and blood levels of copper, selenium, vitamin C and vitamin E in the general population

et al. 2005

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Background: Increased dietary intake of antioxidants has been associated with higher lung function, but few studies have used biological markers of antioxidant intake. Objective: This study aimed to determine if antioxidant status, as measured by blood levels, influences lung function. Design: Using a random subsample of 479 participants, aged 18-65 y old, from a larger cross-sectional observational study, the association of forced expiratory volume in 1 s (FEV 1 ) with plasma copper, vitamin C, vitamin E and serum selenium was assessed. Results: An s.d. increase in blood copper level was associated with a difference in FEV 1 of À48 ml (95% confidence intervals: À95, À2 ml, P ¼ 0.04), vitamin C þ 49 ml ( þ 4, þ 94, P ¼ 0.03), vitamin E À15 ml (À62, þ 32, P ¼ 0.53) and selenium þ 52 ml ( þ 7, þ 96, P ¼ 0.02). The sizes of association were not appreciably altered in a mutually adjusted model. Conclusions: Higher levels of serum vitamin C and selenium appear to be associated with higher FEV 1 . The association between higher serum copper and lower FEV 1 requires further study in view of the ubiquitous exposure to this mineral. Sponsorship: National Asthma Campaign and British Lung Foundation.

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Simultaneous determination of α-tocopherol and retinol in plasma or red cells by high pressure liquid chromatography

Cited by 949 publications

References 12 publications

The effects of cobalt and iodine supplementation of the pregnant ewe diet on immunoglobulin G, vitamin E, T3 and T4 levels in the progeny

The effects of cobalt and iodine supplementation of the pregnant ewe diet on immunoglobulin G, vitamin E, T3 and T4 levels in the progeny

Effects of bovine serum concentrate, with or without supplemental micronutrients, on the growth, morbidity, and micronutrient status of young children in a low-income, peri-urban Guatemalan community

Lung function and blood levels of copper, selenium, vitamin C and vitamin E in the general population

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