Simultaneous determination of Zn, Cd, Pb, and Cu in urine of patients with blackfoot disease using anodic stripping voltammetry

“…Blackfoot disease is a peripheral vascular disease resulting in gangrene of the lower extremities (Pan et al, 1993). The endemic area is along the south-west coast in Taiwan (Tseng et al, 1968;Brown et al, 1995;Tseng et al, 1995).…”

mentioning

confidence: 99%

Mutational spectrum of p53 gene in arsenic-related skin cancers from the blackfoot disease endemic area of Taiwan

et al. 1999

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SummaryTo understand the role of p53 tumour suppressor gene in the carcinogenesis of arsenic-related skin cancers from the blackfoot disease endemic area of Taiwan, we collected tumour samples from 23 patients with Bowen's disease, seven patients with basal cell carcinomas (BCC) and nine patients with squamous cell carcinomas (SCC). The result showed that p53 gene mutations were found in 39% of cases with Bowen's disease (9/23), 28.6% of cases with BCC (2/7) and 55.6% of cases with SCC (5/9). Most of the mutation sites were located on exon 5 and exon 8. Moreover, the results from direct sequencing indicated that missense mutations were found at codon 149 (C→T) in one case, codon 175 (G→A) in three cases, codon 273 (G→C) in three cases, codon 292 (T→A) in one case, codon 283 (G→T) in one case, codon 172 (T→C) in one case and codon 284 (C→A) in one case. In addition, silent mutations were also found in four cases. These mutations were located at codons 174, 253, 289 and 298 respectively. In immunohistochemistry analysis, p53 overexpression was found in 43.5% (10/23) of cases with Bowen's disease, 14% (1/7) of cases with BCC and 44% (4/9) of cases with SSC. These findings showed that p53 gene mutation rate in arsenic-related skin cancers from the blackfoot disease endemic area of Taiwan is high and that the mutation types are different from those in UV-induced skin cancers.

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“…Blood and urine specimens were kept at -20°C prior to analysis. 15 Microwave dissolution technique was used to digest blood samples by a Model-MDS-2000 CEM remote Microwave system (CEM, Matthews, NC). A blood sample (2.5 mL) and 4 mL of the Bi-acid solution (3 mL 65% HNO 3 ϩ 1 mL 30% H 2 O 2 ) were transferred into a 125-mL pressureresistant polytetrafluoroethylene bottle.…”

Section: Collection and Analysis Of Biomonitoring Specimensmentioning

confidence: 99%

Biological Monitoring of Exposures to Aluminium, Gallium, Indium, Arsenic, and Antimony in Optoelectronic Industry Workers

Liao

et al. 2004

Journal of Occupational and Environmental Medicine

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The main objective of this study was to investigate aluminum, gallium, indium, arsenic, and antimony exposures on blood and urine levels in the optoelectronic workers. One hundred seventy subjects were enrolled in this cohort study. Whole blood and urine levels were determined by inductively coupled plasma-mass spectrometry. Blood indium and urine gallium and arsenic levels in the 103 workers were significantly higher than that in 67 controls during the follow-up period. In regression models, the significant risk factors of exposure were job title, preventive equipment, Quetelet's index, sex, and education level. The findings of this study suggest that gallium, indium, and arsenic exposure levels may affect their respective levels in blood and urine. The use of clean, preventive equipment is recommended when prioritizing the administration of safety and hygiene in optoelectronics industries.

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Simultaneous determination of Zn, Cd, Pb, and Cu in urine of patients with blackfoot disease using anodic stripping voltammetry

Cited by 18 publications

References 12 publications

Lipid Peroxidation in Workers Exposed to Aluminium, Gallium, Indium, Arsenic, and Antimony in the Optoelectronic Industry

Lipid Peroxidation in Workers Exposed to Aluminium, Gallium, Indium, Arsenic, and Antimony in the Optoelectronic Industry

Mutational spectrum of p53 gene in arsenic-related skin cancers from the blackfoot disease endemic area of Taiwan

Biological Monitoring of Exposures to Aluminium, Gallium, Indium, Arsenic, and Antimony in Optoelectronic Industry Workers

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