Simultaneous determination of uric and ascorbic acids in human serum by reversed-phase high-performance liquid chromatography with electrochemical detection

Iriyama, Keiji; Yoshiura, Masahiko; Iwamoto, Takeo; Ozaki, Yukihiro

doi:10.1016/0003-2697(84)90451-2

Cited by 122 publications

(62 citation statements)

References 9 publications

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“…Ascorbic acid and uric acid concentrations were determined using a high-performance liquid chromatography (HPLC) method based on that of IRIYAMA et al [14] and as reported recently [15]. A microplate reader adaptation [15] of the enzyme recycling method described by TIETZE [16] was used for the determination of reduced (GSH) and oxidized (GSSG) glutathione.…”

Section: Antioxidant Determinationsmentioning

confidence: 99%

Bronchiolitis obliterans syndrome in lung transplant recipients is associated with increased neutrophil activity and decreased antioxidant status in the lung

Riise

Williams²,

Kjellström³

et al. 1998

Eur Respir J

120

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Long-term survival of lung transplant recipients is limited by the advent of obliterative bronchiolitis and irreversible airways obstruction, e.g. bronchiolitis obliterans syndrome (BOS). This study investigated whether inflammatory cells and their activation markers were increased in bronchoalveolar lavage (BAL) and transbronchial biopsies (TBB) from patients with BOS. Levels of antioxidants in BAL fluid were also assessed. BAL fluid and TBB from six single-lung, two bilateral-lung, and five heart-lung transplanted patients with diagnosis of BOS were compared with 13 transplant recipients without BOS. BAL fluid levels of myeloperoxidase (MPO), eosinophil cationic protein (ECP) and interleukin (IL)-8 were used as markers for the activation and attraction of neutrophils and eosinophils, respectively. Immunohistochemical staining of TBB with monoclonal antibodies to MPO and ECP (EG2) was performed. Significantly increased BAL percentages of neutrophils and levels of MPO were found in patients with BOS. The findings correlated well with the degree of monoclonal staining for MPO in TBB. BAL levels of ECP and IL-8 were significantly increased in BOS patients. BAL concentrations of the water-soluble antioxidants ascorbate, urate and glutathione were generally lower in BOS patients. The results indicate that neutrophil infiltration and activation, as well as oxidative stress, may play a role in the development and/or progression of bronchiolitis obliterans syndrome. Markers for neutrophil activation could have a potential role in monitoring disease activity in patients with this syndrome.

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Section: Antioxidant Determinationsmentioning

confidence: 99%

Bronchiolitis obliterans syndrome in lung transplant recipients is associated with increased neutrophil activity and decreased antioxidant status in the lung

Riise

Williams²,

Kjellström³

et al. 1998

Eur Respir J

120

View full text Add to dashboard Cite

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“…The HPLC determination of uric acid and ascorbate was based on the method of Iriyama et a1 (13). Plasma (100 pL) was extracted with 400 p L of 2% metaphosphoric acid and 100 p L of HPLC-grade heptane.…”

Section: Chemicakmentioning

confidence: 99%

Evidence for Increased oxidative Damage in Patients with Cystic Fibrosis

1994

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Patients with cystic fibrosis (CF) may be more susceptible to oxidative cell injury than normal healthy children due to both the impaired absorption of antioxidant nutrients and the increased oxidative stress caused by chronic pulmonary infections. The purpose of this study was to examine whether markers of oxidative damage to lipids (malondialdehyde-like substances and lipid hydroperoxides) and proteins (protein carbonyls) were present in the plasma of CF patients. Mean values (? SD) of thiobarbituric acid-reactive substances were significantly higher in patients (6.93 & 1.47 prnol/L, n = 25) than in controls (5.84 2 0.59 ~mol/L,; n = 10). FFA hydroperoxides were not detected in control subjects (the detection limit of the assay was 0.02 pmol/L), but in 11 of the 33 CF patients studied they were found in a range of 0.03-0.34 pmoVL. Plasma protein carbonyl concentrations did not differ significantly between the two groups @ = 0.076), although a much wider distribution was observed in the CF patients (range 0.17-5.64 nmollmg protein) than in the control group (range 0.24-1.55 nmollmg protein). No correlation was found between thiobarbituric acid-reactive substances and FFA

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“…Determination of plasma ascorbate and urate levels was carried out using a slight modification of the method of Iriyama et al (20). In brief, 0.1 mL of plasma was mixed with 0.9 mL of an aqueous solution containing 10% metaphosphoric acid and 20 mM deferoxamine mesylate, and the mixture was centrifuged at 3000 X g for 10 min at 4°C.…”

Section: Determination Of Plasma Antioxidantsmentioning

confidence: 99%