Simultaneous Determination of Two Multicomponent Mixtures Containing Phenobarbitone and Ephedrine Hydrochloride Using HPLC and Chemometric Assisted Spectrophotometric Methods

Salam, Randa A. Abdel; Hadad, Ghada M.; Hameed, Eman A. Abdel

doi:10.1080/10826076.2012.657735

Cited by 11 publications

(2 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HPLC is frequently employed as the preferred method for the analysis of ephedrines, a class of alkaloids commonly present in plants such as Ephedra sinica, owing to its high efficiency and sensitivity. There are many studies that have used this method to analyze biological samples to detect ephedrines such as (Abdel Salam et al 2013 ; ’Aksu Donmez et al 2018 ; Kallinteris et al 2022 ). There is a difference between the results of our current study and the study of Kallinteris et al ( 2022 ) (Kallinteris et al 2022 ), as we found that the retention time of ephedrines was 1.63 min, while Kallinteris and his colleagues stated that the retention time was 6.31 min.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of stability of (1R,2 S)-(−)-2-methylamino-1-phenyl-1-propanol hydrochloride in plasma and urine samples-inoculated with Escherichia coli using high-performance liquid chromatography (HPLC)

Almaary,

Alharbi,

Al-Huwaymil

et al. 2024

World J Microbiol Biotechnol

View full text Add to dashboard Cite

The preservation of drug stability in biological evidence during the processes of collection and storage poses a substantial obstacle to the progress of forensic investigations. In conjunction with other constituents, the microorganisms present in the samples play a vital role in this investigation. The present investigation employed the high-performance liquid chromatography (HPLC) technique to assess the stability of (1R,2 S)-(–)-2-methylamino-1-phenyl-1-propanol hydrochloride in plasma and urine samples that were inoculated with Escherichia coli. These samples were subjected to storage conditions of 37 °C for 48 h and − 20 °C for a duration of 6 months. Minimal inhibitory concentration (MIC) and Minimal bactericidal concentration (MBC) of MPPH against E. coli were determined using microdilution method. The stability of MPPH in plasma and urine samples inoculated with E. coli was investigated using HPLC method. The results showed the MIC and MBC of MPPH were 87.5 ± 25 ppm and 175 ± 50 ppm, respectively. While MPPH remained stable in plasma for 48 h at 37 °C, it showed a notable decrease of about 11% in stability when stored in urine for the same period and temperature. From the beginning of the first month, a decrease in the stability of the compound appeared in all samples that were stored at − 20 °C, and the decrease reached 7% for plasma samples and about 11% for urine samples. The decrease in the stability reached its peak in the sixth month, reaching more than 30% and 70% of plasma and urine samples preserved at − 20 °C. This work concluded that E. coli can negatively affect the stability of MPPH in plasma and urine samples. This may lead to incorrect conclusions regarding the analysis of biological samples in criminal cases.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of stability of (1R,2 S)-(−)-2-methylamino-1-phenyl-1-propanol hydrochloride in plasma and urine samples-inoculated with Escherichia coli using high-performance liquid chromatography (HPLC)

Almaary,

Alharbi,

Al-Huwaymil

et al. 2024

World J Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…HPLC, a chemical analysis method, has long been recognized as a reliable method for analyzing ephedrine in biological samples. For example, extensive research has established its suitability for this purpose such as ( Abdel Salam et al, 2013 , Aksu et al, 2018 , Kallinteris et al, 2022 ). The results of previous studies and our current study did not completely match due to differences in samples, extraction methods, mobile phase, and other differences.…”

Section: Discussionmentioning

confidence: 99%