Simultaneous determination of twelve benzodiazepines in human serum using a new reversed-phase chromatographic column on a 2-μm porous microspherical silica gel

Tanaka, Einosuke; Terada, Masaru; Misawa, Shogo; Wakasugi, Choei

doi:10.1016/0378-4347(96)00121-1

Cited by 56 publications

(25 citation statements)

References 12 publications

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“…The measurement of blood concentration of propofol was performed according to previously described methods [14, 15]. For the measurement of propofol, acetnitrile and internal standard added to a plasma sample and vortexed for 1 min.…”

Section: Methodsmentioning

confidence: 99%

Safety and Driving Ability following Low-Dose Propofol Sedation

Horiuchi¹,

Nakayama

Katsuyama

et al. 2008

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Background and Aim: Automobile driving is prohibited after midazolam sedation because of the slow recovery of psychomotor function. This study prospectively assessed the safety of low-dose propofol sedation (study 1) and compared driving ability following propofol and midazolam sedation (study 2). Methods: Study 1: We prospectively investigated bolus injection of a low-dose of propofol (40–80 mg for <70 years and 30 mg for ≥70 years) for diagnostic esophagogastroduodenoscopy (EGD). Respiratory depression, time to full recovery, and overall patient satisfaction were evaluated and blood concentrations of propofol were measured. Study 2: A subset of subjects undergoing diagnostic EGD were randomized to receive 40 mg of propofol (n = 30), 4 mg of midazolam (n = 30) or no sedation controls (n = 20), and the residual effects of each drug were tested using a driving simulator. The primary outcome measure was driving ability. The second outcome measures were overall patient satisfaction and complications. Results: Study 1: Only 1.1% of 12,031 healthy subjects developed transient oxygen desaturation. Full recovery was present in 97.5% 30 min after the procedure; 99.8% were willing to repeat the same procedure. The blood levels of propofol (40–80 mg) at 60 min were <100 ng/ml. Study 2: Driving ability recovered to the basal level within 60 min of propofol administration but not with 120 min with midazolam. There were no complications; overall patient satisfaction was similar between propofol and midazolam (8.9 vs. 8.5, p = 0.34). Conclusion: Low-dose propofol sedation was safe and recovery including driving ability was with 60 min.

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Section: Methodsmentioning

confidence: 99%

Safety and Driving Ability following Low-Dose Propofol Sedation

Horiuchi¹,

Nakayama

Katsuyama

et al. 2008

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“…GC/ MS-selected ion monitoring (SIM), 0.1 ng on column [5]; underivatized oxazolam, haloxazolam, mexazolam, cloxazolam, and flutazolam in whole blood by BPX-5 capillary column (15 m × 0.32 mm i.d.) GC/MS-SIM method, 5, 20, 1, 20, and 5 ng/ml, respectively [9]; oxazolam, haloxazolam, and flutazolam in serum by HPLC, 5, 50, and 10 ng/ml, respectively [11]. In the present study, the LODs of underivatized and methylated oxazolobenzodiazepines with the nonpolar DB-1 wide-bore column (15 m × 0.53 mm i.d.)…”

Section: Sensitivitymentioning

confidence: 52%

“…Various methods for the determination of benzodiazepines in biological materials have been reported. Analytical methods for underivatized oxazolobenzodiazepines and other benzodiazepines include thin-layer chromatography (TLC) [2,3], high-performance (HP) TLC [4], gas chromatography (GC) [3,[5][6][7], GC-mass spectrometry (MS) [3,5,[7][8][9], high-performance liquid chromatography (HPLC) [3,10,11], and immunoassays [12]. However, simultaneous determination of methylated oxazolobenzodiazepine drugs by GC or GC/MS has not been reported to our knowledge.…”

Section: Introductionmentioning

confidence: 99%

Rapid and simple analysis of oxazolobenzodiazepine drugs in sera by wide-bore capillary gas chromatography with nitrogen-phosphorus detection using on-column methylation

et al. 2006

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“…A number of analytical HPLC methods for the simultaneous determination of benzodiazepines in biological matrices have been published [54]. Among these, most common UV detection [50,55] with the detection of limit of 2 ng/ml, while one method reaches 1 ng/ml in analyzing post-mortem blood [56] and applied other detection method such as spectorphotometric and fluorimetric method [57] and recently, LC-MS method have been also described [58][59][60][61].…”

Section: Clonazepam (Czp)mentioning

confidence: 99%